Application of trichoderma acid in preparation of antifungal medicines
An antifungal drug, a technology of trichoderma, applied in the field of medicine and biology, can solve problems such as reports on the antifungal activity of trichoderma, and achieve the effects of good application and development prospects, good antifungal activity, and strong inhibitory effect.
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Embodiment 1
[0017] The antibacterial activity of trichoderma acid against the pathogenic bacteria Candida albicans was determined by the filter paper method.
[0018] The compound Trichoderma acid was dissolved in DMSO and diluted to 500 μg / mL.
[0019] Candida albicans (Candida albicans) standard strain B7 (ATCC10231) and clinical strain BB2 obtained by liquid fermentation were diluted to 10 with normal saline, respectively. 6 The concentration of CFU / mL, the absorption concentration is 10 6 Put 1mL of CFU / mL bacterial solution into the plate, pour into the PDA medium that has been cooled to an appropriate temperature, and mix well. Use sterile tweezers to pick a filter paper sheet with a thickness of 1.5mm and a diameter of 6mm and place it on a flat plate containing bacteria. At the same time, 5 μL of Trichoderma acid was added dropwise on the filter paper sheet as a sample group, and DMSO was used instead of Trichoderma acid as a blank control group. Nystatin with a concentration of...
Embodiment 2
[0025] The minimum inhibitory concentration of Trichoderma acid against Candida albicans was determined by the filter paper method.
[0026] Trichodermaic acid or nystatin were diluted with DMSO to 100, 90, 80, 70, 60, 50, 40, 30, 20 μg / mL, respectively. The absorption concentration is 10 6 Put 1 mL of CFU / mL Candida albicans (standard strain B7 (ATCC10231) or clinical strain BB2) bacterial solution into the plate, pour into the PDA medium that has been cooled to an appropriate temperature, and mix well. Use sterile tweezers to pick up a filter paper sheet with a thickness of 1.5 mm and a diameter of 6 mm and place it on a flat plate containing bacteria. At the same time, add 5 μL of the above-mentioned concentration of trichoderma acid or nystatin to the filter paper sheet, and place it in a 26°C incubator for incubation. After 36 hours, observe the bacteriostatic zone around the filter paper, and take the minimum concentration of the sample contained in the filter paper wit...
Embodiment 3
[0032] The growth confrontation method was used to determine the inhibitory effect of Trichoderma acid on the plant pathogenic fungus Gleospora anthracnose.
[0033] Dissolve and dilute Trichoderma acid to 1000 µg / mL with DMSO.
[0034] Insert a bacterium cake with a diameter of 4mm in the center of the PDA medium, and then place a filter paper sheet with a diameter of 6mm at a distance of 1.5mm from the bacterium cake, and drop 5 μL of the above-mentioned Trichoderma acid dilution on the filter paper, repeat 3 The second time, cultured at 26°C for 5 days, replaced Trichodermalic acid with DMSO as a blank control, measured the diameter of the test bacteria cake with the cross measurement method, and calculated the inhibition diameter.
[0035] Bacterial inhibition diameter (mm) = growth diameter of bacteria cake in blank control group - growth diameter of bacteria cake in treatment group
[0036] The experimental results are shown in Table 3:
[0037] Table 3: Inhibitory eff...
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