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Preparation method of low-molecular-weight sea cucumber fucosan sulfate

A technology of fucoidan sulfate and low molecular weight, which is applied in the field of preparation of low molecular weight sea cucumber fucoidan sulfate, can solve the problems of severe molecular structure damage, difficulty in industrialized large-scale production, harsh reaction conditions, etc., and achieve good The ability to scavenge free radicals, good anticoagulant activity, and little damage to the product structure

Active Publication Date: 2013-05-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biodegradation method has mild reaction and high specificity, enzymes that can be applied to polysaccharides, especially for the degradation of fucoidan sulfate, are very rare, and the reaction conditions are relatively harsh, and the cost is high. must be difficult
The physical degradation method has become an ideal degradation method due to its small damage to the active structure of the product, easy control of the molecular weight, and high reaction efficiency. Commonly used physical degradation methods for polysaccharides include ultrasonic degradation, irradiation degradation, and microwave degradation. Among them, irradiation The degradation efficiency is the highest. The research on konjac glucomannan shows that after irradiation of 5.0kGy and 100.0kGy, the molecular weight of the product is reduced from 481kDa to 370kDa and 39.8kDa respectively, but the molecular structure is severely damaged by irradiation treatment. And the security is still controversial, so its application is limited

Method used

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  • Preparation method of low-molecular-weight sea cucumber fucosan sulfate
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  • Preparation method of low-molecular-weight sea cucumber fucosan sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0027] Example 1-1. A method for preparing low-molecular-weight sea cucumber fucoidan sulfate, using sea cucumber polysaccharides obtained by enzymatic hydrolysis after pulverizing sea cucumbers as raw materials, and the following steps are carried out in sequence:

[0028] 1) Dissolve sea cucumber polysaccharides in a 3mol / L sodium chloride solution (concentrated saline) to obtain a sea cucumber polysaccharide solution. The mass concentration of sea cucumber polysaccharide in the sea cucumber polysaccharide solution is 1%;

[0029] Add ethanol to the sea cucumber polysaccharide solution until the final concentration of ethanol is 37%. Centrifuge (6000r / min, 15min) to remove the precipitate to obtain the supernatant;

[0030] Add ethanol to the supernatant until the final concentration of ethanol is 75%, centrifuge (6000r / min, 15min), and the resulting precipitate is dialyzed (over a dialysis bag with a molecular weight cut-off of 8000kDa) and freeze-dried (in- After drying at 45°C a...

Embodiment 1-2~1-5

[0035] In order to verify the effect of ultrasonic degradation of different ultrasonic sound field intensity in step 2) on the prepared low-molecular-weight sea cucumber fucoidan sulfate, the ultrasonic sound field intensity in step 2) was changed, and the rest were the same as in Example 1-1 to obtain Examples 1-2 to 1-5; the specific ultrasonic sound field intensity and the corresponding molecular weight of the obtained low molecular weight sea cucumber fucoidan sulfate are shown in Table 1.

[0036] Table 1. Molecular weight of low molecular weight sea cucumber fucoidan sulfate obtained from different ultrasonic sound field intensity

[0037]

[0038] It can be found from Table 1 that the ultrasonic sound field intensity is 181.53-544.59 W / cm 2 After 10 minutes of action, with the increase of ultrasonic sound field intensity, the molecular weight of sea cucumber fucoidan sulfate continued to decrease, focusing on 138.47-217.48kDa. When the ultrasonic sound field intensity was 54...

Embodiment 2-1~2-3

[0040] In order to verify the effect of ultrasonic degradation at different reaction temperatures in step 2) on the prepared low-molecular-weight sea cucumber fucoidan sulfate, the reaction temperature in step 2) of Example 1-4 was changed, and the rest were the same as in Example 1. -4, so as to obtain Examples 2-1~2-3 (that is, the processing time is 10 minutes, the ultrasonic sound field intensity is 423.57 W / cm 2 ); The specific reaction temperature and the corresponding molecular weight of low molecular weight sea cucumber fucoidan sulfate are shown in Table 2.

[0041] Table 2. Molecular weight of low molecular weight sea cucumber fucoidan sulfate obtained at different reaction temperatures

[0042]

[0043] It can be found from Table 2 that when the reaction temperature is 5-45°C, after 10 minutes of ultrasonic action, the degradation trend increases as the temperature decreases. The molecular weight of sea cucumber fucoidan sulfate is concentrated in 139.06-212.97kDa. Wh...

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Abstract

The invention discloses a preparation method of low-molecular-weight sea cucumber fucosan sulfate. In the preparation method, a sea cucumber polysaccharide prepared by performing enzymolysis on pulverized sea cucumbers is used as a raw material. The preparation method comprises the following steps: 1) dissolving the sea cucumber polysaccharide in a sodium chloride solution, then adding ethanol, and centrifuging to remove the precipitate, thus obtaining the supernatant; and adding ethanol into the supernatant, centrifuging, dialyzing the obtained precipitate, and performing freeze-drying to obtain sea cucumber fucosan sulfate; 2) dissolving the sea cucumber fucosan sulfate in solvent, and performing ultrasonic treatment at 0-45 DEG C, wherein the treatment time is 10-180 minutes, and the ultrasonic sound field strength is 60-800 W / cm<2>; and 3) dialyzing the treated solution obtained in the step 2), and performing vacuum freeze-drying to obtain the low-molecular-weight sea cucumber fucosan sulfate. According to the invention, an ultrasonic degradation technology is adopted, high-molecular-weight sea cucumber fucosan sulfate can be quickly degraded, the reaction conditions are mild, and the preparation method is green and environment-friendly.

Description

Technical field [0001] The invention relates to a method for preparing low-molecular-weight sea cucumber fucoidan sulfate, and to be precise, relates to a method for preparing low-molecular-weight sea cucumber fucoidan sulfate by ultrasonic degradation. Background technique [0002] Sea cucumber (holothurians) belongs to the Echinodermata (Echinodermata) Holothuroidea (Holothuroidea) and Aspidocita animals. They are mainly distributed in tropical and temperate oceans. There are 101 species of sea cucumbers recorded in my country, of which more than 20 are edible . Since ancient times, sea cucumber has been a valuable nourishing product for both medicine and food, ranking first in the "sea". Traditional Chinese medicine believes that sea cucumber is salty, warm in nature, enters the heart and kidney meridian, and has the function of nourishing blood, moisturizing dryness, and stopping bleeding. Anti-inflammatory, and stomach thirst quenching effect. In recent years, researches be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 陈士国郭欣叶兴乾孙玉敬刘东红
Owner ZHEJIANG UNIV
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