Device and method for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria

Abstract

The invention discloses a device and a method for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria. The method comprises the following steps of: arranging a device for fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria; placing a certain amount of washed and filtered aerobic active sludge into a trench type biological reaction tank, and mixing the aerobic active sludge with a nutrient solution; gradually introducing the nutrient solution heated by a preheating system into the reaction tank; intercepting anaerobic ammonium oxidation bacteria through cloth-type suspension fiber filler; and after 60-90 days culture, taking out the cloth-type suspension fiber filler full of brown anaerobic ammonium oxidation bacteria, eluting and naturally precipitating to obtain a great amount of anaerobic ammonia oxidation denitrified sludge with high purity. The device and method disclosed by the invention can achieve the aims of fast enrichment multiplication and purifying culture of anaerobic ammonium oxidation bacteria so as to provide sufficient inoculated sludge for starting an anaerobic ammonia oxidation denitrifying reactor and achieve the aim of quickly starting the oxidation reactor to remove ammonia nitrogen and nitrite nitrogen out of wastewater.

Description

technical field [0001] The invention relates to environmental biotechnology, in particular to a device and method for rapidly enriching, multiplying, purifying and cultivating anammox bacteria. Background technique [0002] The existence of anammox bacteria was first discovered in 1995. Completely different from previously known nitrifying-denitrifying organisms, this newly discovered ancient bacterium is a chemoautotrophic bacterium that completes denitrification with a new metabolic pathway, that is, under anaerobic conditions , with ammonia nitrogen as the electron donor, nitrite nitrogen as the electron acceptor, and finally generate nitrogen gas. The chemical energy released in this process is used by the bacteria as an energy source for the fixation of inorganic carbon and other tasks. life activity process. Under the conditions of using the anaerobic ammonium oxidation denitrification process, only about half of the ammonia nitrogen in the wastewater needs to be oxi...

AI Technical Summary

Problems solved by technology

However, many studies start the experimental anammox denitrification reactor by directly using activated sludge from aerobic sewage treatment plant, granular sludge from anaerobic sewage treatment or pond bottom sludge to start the reactor. Although the anammox denitrification process can also be carried out, the content of anammox bacteria in the reactor sludge is relatively low, and the total amount of sludge is also relatively small, so it cannot meet the needs of large reactors for anaerobic denitrification. The need for nitrogen activated sludge cannot be applied to production

[0006] Chinese patent application CN102145940A discloses "a device and method for rapidly proliferating anammox bacteria" (1), and CN102061281A discloses "a method for enriching and cultivating anammox bacteria using a membrane bioreactor" (2), CN101735947A discloses "a soil anammox bacteria enrichment system simulating a natural environment" (3). These methods either focus on the rapid proliferation of anammox bacteria, that is, they are inoculated with a small amount of enriched and purified Anammox bacteria (1); either use a membrane bioreactor to enrich and cultivate anammox bacteria, so that dispersed anammox bacteria can be enriched on the membrane, which greatly improves the enrichment efficiency. For membrane organisms There are no specific requirements for the structure of the reactor, and the quantity and purity of the obtained anammox bacteria are not guaranteed (2); or enrich the anammox bacteria by using a soil column to enrich the anammox bacteria In the soil column, there is a problem that it is difficult to separate the enriched bacteria after the enrichment is completed, and it is impossible to directly and conveniently obtain anammox activated sludge with a large quantity and high purity. Therefore, in practical application There are certain restrictions in

Images