Method capable of rapidly evaluating extent of low temperature injury of straw mushroom

A technology for low temperature injury and straw mushroom, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of lack of physiological and biochemical methods, affecting the in-depth study of the low temperature response mechanism of straw mushroom, etc., to shorten the breeding cycle, easy to master, Measure the effectiveness of reliable data

Active Publication Date: 2013-05-15
SHANGHAI ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although low temperature-induced genes have been reported at the molecular level, there is still a lack of a physiological and biochemical method for quickly identifying the degree of low-temperature injury of V.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method capable of rapidly evaluating extent of low temperature injury of straw mushroom
  • Method capable of rapidly evaluating extent of low temperature injury of straw mushroom
  • Method capable of rapidly evaluating extent of low temperature injury of straw mushroom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of culture medium:

[0029] (1) Preparation of PDA solid medium: Potato (juice) 200g / L, glucose 20g / L, agar strip 20g / L, added to distilled water, mixed evenly, put into a triangular flask, and sterilized at 121°C for 20 minutes; Cool to 45~50°C, pour into a sterilized petri dish with a diameter of 9cm, the pouring amount is 35% of the volume of the petri dish, solidify for later use. The specific operation of potato juice extraction is as follows: select potatoes, clean the surface, peel them, and weigh 200g; cut the potatoes into pieces, add distilled water and boil them (generally boil for 20-30min, and it can be pierced by a glass rod) , filtered with four layers of gauze.

[0030] (2) Preparation of PDY liquid medium: Potato (juice) 200g / L, glucose 20g / L, yeast extract 2g / L, added to distilled water, after being completely dissolved, divided into triangular flasks, each bottle 100ml, in Sterilize at 121°C for 20 minutes. The operation steps of pot...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method capable of rapidly evaluating an extent of low temperature injury of straw mushroom. The method capable of rapidly evaluating the extent of low temperature injury of the straw mushroom includes: (1) inoculating activated straw mushroom bacterial strains on a sterile personal digital assistant (PDA) culture medium, culturing for three to four days through static culture; transferring hypha to PDY culture solution after the hypha overgrows a plate, culturing for three to four days through shake cultivation; (2) filtering the hypha in the PDY culture solution, conducting low-temperature treatment, respectively taking hypha balls to conduct vibration under temperature of twenty to twenty-five DEG C at each low-temperature treatment time point; determining an initial conductivity E1, sterilizing again, vibrating, determining a final conductivity E2; and evaluating the extent of low temperature injury of straw mushroom according to a calculated result. The method capable of rapidly evaluating the extent of low temperature injury of the straw mushroom is easy and convenient to operate and easy to master, enables the identification of the extent of low temperature injury of the straw mushroom of a whole system to be conducted under a controllable condition, enables determined data to be reliable, is capable of being used in rapid judgment of low temperature resistance among different straw mushroom bacterial strains and is capable of greatly reducing a breeding period of screening low temperature resistant mutant strains.

Description

technical field [0001] The invention belongs to the field of evaluating the degree of low temperature damage to edible fungi, in particular to a method for quickly evaluating the degree of low temperature damage to straw mushrooms. Background technique [0002] Straw mushroom (Volvariella volvacea (Bull ex Fr.) Sing), also known as straw mushroom, orchid mushroom, and Chinese mushroom, belongs to Basidiomycetes, Agaricales, Phyllosporaceae, and Physophthora genus. Straw mushroom is delicious and nutritious, and it is a high-quality mushroom species in edible fungi. According to research reports, fresh straw mushroom contains 92.39% water, 2.66% protein, 2.24% fat, 1.66% reducing sugar, 0.95% invert sugar, and 0.91% ash; there are 18 kinds of amino acids in the straw mushroom protein, including 8 that cannot be synthesized by the human body. an essential amino acid. In addition to edible value, straw mushrooms also have the effects of improving human immunity, inhibiting th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04G01N27/04
Inventor 赵妍陈明杰姜威汪虹张宝粉
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap