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Interdigital electrode with nano-dot array, and preparation method and application thereof

A nano-dot array and interdigital electrode technology, which is applied in the direction of material analysis, material inspection products, and measurement devices by electromagnetic means, can solve the problems of insufficient detection sensitivity, small size, and difficult electrode gap of interdigital electrode biosensors. , to achieve the effect of improving accuracy and sensitivity, good compatibility and low cost

Active Publication Date: 2013-05-15
NAT UNIV OF DEFENSE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these interdigital electrode structures are manufactured by conventional photolithography printing method, the electrode gap is difficult to achieve a small size, and the detection sensitivity of the interdigital electrode biosensor is not high enough.

Method used

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  • Interdigital electrode with nano-dot array, and preparation method and application thereof
  • Interdigital electrode with nano-dot array, and preparation method and application thereof
  • Interdigital electrode with nano-dot array, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1: Interdigital electrodes with nano-dot arrays and their application in the preparation and detection of thyrotropin.

[0039] a kind of like figure 1 with Figure 11 The shown interdigitated electrode with a nano-dot array of the present invention, the interdigitated electrode is based on a metal interdigitated electrode array 1, and a metal nano-dot structure array 2 is implanted in the gap of the metal interdigitated electrode array 1, The metal nano-dot structure array 2 is in a hexagonal arrangement. The gap distance a of the metal interdigitated electrode array 1 is 10 μm, and the metal nano-dot structure array 2 is uniformly distributed in the gap of the metal interdigitated electrode array 1 . Between the metal interdigitated electrode array 1 and the metal nano-dot structure array 2 (and between the metal nano-dot structure arrays 2 ), a cylindrical spacer array 3 composed of a plurality of cylindrical cavities is formed. The array unit gap b of t...

Embodiment 2

[0054] Example 2: Interdigital electrode with nano-dot array and its preparation and application for detection of Staphylococcus aureus enterotoxin.

[0055] In this embodiment, the interdigitated electrode with a nano-dot array described in Embodiment 1 is used; its preparation process is the same as that of Embodiment 1.

[0056] The interdigitated electrode with the nano-dot array prepared in Example 1 is used to detect Staphylococcus aureus enterotoxin, and the specific detection method comprises the following steps:

[0057] (1) Preparation of biosensors: The interdigitated electrodes with nanodot arrays prepared above were thoroughly cleaned, and then placed in an ethanol solution containing 5% (volume percent) of aminopropyltrimethoxysilane, at room temperature Stand still for 24 hours; immerse the silanized interdigitated electrode containing the nano-dot array in 5% glutaraldehyde aqueous solution, and let it stand for 1 hour at room temperature; The capture antibo...

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Abstract

The invention discloses an interdigital electrode with a nano-dot array, wherein a metal interdigital electrode array is taken as the main body, and a metal nano-dot structure array is implanted into the gap of the metal interdigital electrode array. The preparation method of the interdigital electrode with the nano-dot array provided by the invention comprises the following steps of: firstly, forming a single layer of ordered dense polystyrene nanosphere arrangement on the surface of a glass sheet; secondly, depositing a metal film on the dense arrangement of polystyrene nanospheres, wherein the deposition thickness of the metal film is lower than 1 / 2 of the height of the nanosphere; and then removing the nanospheres to obtain the metal nano-dot array; depositing the metal film on the metal nano-dot array, forming an electrode pattern outlined by photoresist on the surface of the metal film, and then performing wet-process corrosion until the metal nano-dot array is exposed again, thereby obtaining the interdigital electrode with the nano-dot array. The interdigital electrode provided by the invention can be used as a biosensor, and is high in compatibility, high in efficiency, low in cost, high in sensitivity and the like.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, and in particular relates to an interdigital electrode which can be used for detection of biochemical substances and its preparation method and application. Background technique [0002] In recent years, with the continuous improvement of people's living standards, people have paid more and more attention to the field of food safety. Food safety issues such as melamine, clenbuterol, gutter oil, and pesticide residues in fruits and vegetables have also attracted much attention. It is urgent to monitor their own food safety. Accurate discrimination requires a fast and effective detection method for biochemical substances. In addition, in the medical field, early diagnosis of cancer and other diseases, isolation and detection of infectious bacteria; in the field of environmental monitoring, water pollution of rivers and lakes, production and domestic sewage discharge monito...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/78G01N33/543
Inventor 吴学忠董培涛陈剑王浩旭王朝光王俊峰
Owner NAT UNIV OF DEFENSE TECH
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