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Phosphorite hollow microsphere and preparation method thereof

A technology of apatite and microspheres, which is applied to the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., can solve problems such as ignoring the effective control of uniform distribution, affecting the biological properties of materials, and affecting the release of strontium elements, etc., to achieve Effect of improving biological performance and good cytocompatibility

Active Publication Date: 2013-05-29
广州康睿医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many preparation methods of strontium-doped apatite, such as solid-state calcination method, sol-gel method, hydrothermal method, etc. The strontium-doped process of these traditional methods is relatively straightforward and simple. The form of strontium is directly added to the synthesis process, but the content of strontium element involved in life activities is quite small, and many traditional processes often ignore the effective control of the trace amount of strontium doped and its uniform distribution in the material. To a certain extent, it affects the release of strontium in the application process, which in turn affects the biological properties of the material

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  • Phosphorite hollow microsphere and preparation method thereof
  • Phosphorite hollow microsphere and preparation method thereof
  • Phosphorite hollow microsphere and preparation method thereof

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Effect test

Embodiment 1

[0030] A biomimetic preparation method of apatite hollow microspheres, the steps of which are as follows:

[0031] (1) Inoculate baker's yeast (Saccharomyces cerevisiae) in yeast extract powder peptone glucose medium (YPD) (2% peptone, 2% glucose, 0.5% yeast extract), and shake for 3 days at a speed of 180 rpm Minutes, enriched yeast cells were obtained after centrifugal purification;

[0032] (2) The yeast cells obtained above were immersed in 1wt% polydimethyldiallyl ammonium chloride aqueous solution and 1wt% polyacrylic acid aqueous solution at a ratio of 5wt%, each of which was continuously stirred for 20 minutes, and the above steps were repeated Once, the bio-templates processed by layer-by-layer self-assembly are obtained;

[0033] (3) Immerse the above-mentioned biological template in calcium chloride (3M) aqueous solution, stir continuously at room temperature for 24 hours, and obtain the biological template adsorbing metal ions after separation;

[0034] (4) Immer...

Embodiment 2

[0037] A biomimetic preparation method of apatite hollow microspheres, the steps of which are as follows:

[0038] (1) Inoculate the bread strain (Saccharomyces cerevisiae) in yeast extract powder peptone glucose medium (YPD) (2wt% peptone, 2wt% glucose, 0.5wt% yeast extract), and shake for 3 days at a speed of 180 rev / min, enriched yeast cells were obtained after centrifugation and purification;

[0039] (2) The yeast cells obtained above were immersed in 1wt% polydimethyldiallyl ammonium chloride aqueous solution and 1wt% polyacrylic acid aqueous solution at a ratio of 5wt%, each of which was continuously stirred for 20 minutes, and the above steps were repeated Once, the bio-templates processed by layer-by-layer self-assembly are obtained;

[0040] (3) Immerse the above-mentioned biological template in calcium chloride (3M) aqueous solution, stir continuously at room temperature for 24 hours, and obtain the biological template adsorbing metal ions after separation;

[004...

Embodiment 3

[0044]A biomimetic preparation method of apatite hollow microspheres, the steps of which are as follows:

[0045] (1) Inoculate baker's yeast (Saccharomyces cerevisiae) in yeast extract powder peptone glucose medium (YPD) (2wt% peptone, 2wt% glucose, 0.5wt% yeast extract), shake and culture for 3 days at 180 rpm / min, the enriched yeast cells were obtained after centrifugation and purification;

[0046] (2) The yeast cells obtained above are immersed in 1wt% polydimethyldiallyl ammonium chloride aqueous solution and 1wt% polyacrylic acid aqueous solution at a ratio of 5wt%, each of which is continuously stirred for 20 minutes, and the above steps are repeated Once, the bio-templates processed by layer-by-layer self-assembly are obtained;

[0047] (3) Immerse the above-mentioned biological template in calcium chloride (3M) aqueous solution, stir continuously at room temperature for 24 hours, and obtain the biological template adsorbing metal ions after separation;

[0048] (4...

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Abstract

The invention discloses a phosphorite hollow microsphere and a preparation method thereof. The preparation method comprises the following steps of incubating a yeast cell into yeast extract powder peptone dextrose culture medium containing 0-10mg / ml of strontium salt to shake and cultivate for 1-3 days, obtaining the gathered yeast cell after centrifugal purification; obtaining a self-assembled biological template from the yeast cell by a layer-by-layer self-assembling technology; soaking the biological template into mixed solution of which the molar ratio of calcium salt to the strontium salt is as follows: Sr / (Ca+Sr)=0-15%; continuously agitating, and obtaining the biological template of adsorbing metal ions after separation; soaking the biological template into phosphorus salt solution; adjusting the pH value to agitate, separating and drying to obtain the composite microsphere; carrying out heat preservation on the composite microsphere for 1-6 hours at 100-900 DEG C to obtain the phosphorite hollow microsphere. The microsphere has a porous hollow structure and good cell compatibility, and can be prepared into a drug carrier or a human tissue engineering bracket and applied to the field of medical treatment of bone tissue defect.

Description

technical field [0001] The invention belongs to the field of bionic material preparation and specifically designs a preparation method of apatite hollow microspheres. Background technique [0002] With the rapid development of biomaterials, biomimetic technology has gradually been widely used to synthesize biomaterials with specific components and morphologies, so that their properties can be closer to natural tissues. Apatite material is one of the most important inorganic biomaterials. It has been widely used due to its similarity to the composition and structure of natural bone and tooth minerals, good biological activity, biodegradability and biocompatibility. in bone tissue engineering and drug delivery systems. There are a variety of trace elements in the inorganic minerals of natural bone tissue. These trace elements play an important role in the interaction between the material and the host. The trace elements can not only effectively improve the physical and chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C01B25/32
Inventor 王迎军黄淼俊赵娜如杜昶
Owner 广州康睿医疗器械有限公司