Method for simultaneously determining contents of 10 nucleoside components in sowthistle-leaf ixeris seedling injection by utilizing HPLC-DAD (High Performance Liquid Chromatography-Diode Array detector) method

A technology of Kudiezi and injection, which is applied in the field of component analysis of traditional Chinese medicine, can solve the problems of high requirements for instruments and poor specificity, and achieve the effects of good repeatability, easy operation and accurate results

Active Publication Date: 2013-06-12
TONGHUA HUAXIA PHARMA
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Problems solved by technology

The specificity of ultraviolet method and thin layer method is poor, and liquid-mass method and capillary electrophoresis method have higher requirements for instruments

Method used

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  • Method for simultaneously determining contents of 10 nucleoside components in sowthistle-leaf ixeris seedling injection by utilizing HPLC-DAD (High Performance Liquid Chromatography-Diode Array detector) method
  • Method for simultaneously determining contents of 10 nucleoside components in sowthistle-leaf ixeris seedling injection by utilizing HPLC-DAD (High Performance Liquid Chromatography-Diode Array detector) method

Examples

Experimental program
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Effect test

Embodiment

[0032] Method for simultaneous determination of 10 nucleoside components in Kudiezi injection by HPLC-DAD method:

[0033] 1 Instruments and reagents

[0034] 1.1 Instrument

[0035] Waters-e2695-HPLC system (including quaternary gradient pump, online vacuum degasser, autosampler, column thermostat, 2998 photodiode array detector PDA, Empower 2 chromatography workstation), Sartorius BT125D analytical balance (Sartorie Si Scientific Instrument (Beijing) Co., Ltd.), KH 2200B Ultrasonic Cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.)

[0036] 1.2 Reagent

[0037] Cytosine reference substance (batch number: C94998BGF0, Tianjin Sino Biochemical Technology Co., Ltd.); uracil reference substance (batch number: 100469-200401, China Institute for the Control of Pharmaceutical and Biological Products); cytidine reference substance (batch number: 101056874, SIGMA company) ; Guanine reference substance (batch number: HCCTH-BG, Shanghai Chemical Industry Development Co., Ltd....

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Abstract

The invention relates to a method for simultaneously determining contents of 10 nucleoside components in sowthistle-leaf ixeris seedling injection by utilizing an HPLC-DAD (High Performance Liquid Chromatography-Diode Array detector) method, and belongs to the field of traditional Chinese medicine component analysis. The method comprises the following steps of: (1) preparation of a mixed reference liquid; (2) preparation of a test liquid; and (3) determining method: precisely absorbing the mixed reference liquid and the test liquid respectively by the HPLC-DAD method, injecting to a detector and detecting, wherein the chromatographic condition is as follows: the chromatographic column is DiamonsilC18(2), the detection wavelength is 260nm, the flow rate is 1mL/min, the column temperature is 35 DEG C, the sample size is 10 microlitres, and the gradient elute condition is 0-5 minutes; performing gradient eluting based on 2% of acetonitrile by volume fraction; linearly increasing to 9% of acetonitrile with 5-9 minutes; linearly increasing to 11% of acetonitrile with 9-15 minutes; and linearly increasing to 40% of acetonitrile with 15-25 minutes. The method for simultaneously determining the contents of the 10 nucleoside components in sowthistle-leaf ixeris seedling injection established by the invention is simple in analytic method, good in repeatability and accurate and reliable, and can provide reference for quality control of the sowthistle-leaf ixeris seedling injection.

Description

[0001] technical field [0002] The invention relates to a method for simultaneously determining the contents of 10 nucleoside components in Kudiezi injection by HPLC-DAD method. Belongs to the field of traditional Chinese medicine component analysis. Background technique [0003] In the prior art, Kudiezi has another name called Gypsophila gypsophila, and it is mainly distributed in Northeast my country and Inner Mongolia. Grass, cold in nature, bitter and pungent in taste, has the effects of clearing heat and detoxifying, draining pus and relieving pain [1] . Kudiezi (Diemailing) Injection is an intravenous injection made from Kudiezi extracted and refined, which has the functions of promoting blood circulation, removing blood stasis and improving microcirculation [2] . The injection has been on the market for many years, and its curative effect is definite. Experimental studies have shown that the chemical composition of Kudiezi injection is relatively complex, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 刘睿马思萌任晓亮李遇伯张艳军
Owner TONGHUA HUAXIA PHARMA
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