Application of bacteroides fragilis in preparation of composition for treating bacterial vaginitis

A technology of Bacteroides fragilis and composition, applied in application, food preparation, antibacterial drugs, etc.

Inactive Publication Date: 2013-06-19
广东知光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Bacteroides fragilis is used as a kind of probiotics, and the relevant application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Bacteroides fragilis CGMCC No.7280 bacterial liquid

[0018] (1) Isolation of Bacteroides fragilis CGMCC No.7280 from infant feces

[0019] Weigh 0.5 g of stool according to the "normal flora test method", put it in a test tube filled with 4.5 ml of normal saline, and serially dilute it to 10 -1 ~10 -6 , from high concentration to low concentration on the Bd medium plate, placed in an anaerobic gas box and anaerobic gas bag for anaerobic culture. Store at 37°C, open the can for inspection after 2-7 days. A single colony was selected for pure culture, staining microscopy, culture characteristics, and physiological and biochemical testing.

[0020] (2) Bacteroides fragilis CGMCC No.7280 enrichment culture and bacterial liquid preparation

[0021] Select the purified single colony and inoculate it in the improved tryptone broth for enrichment culture. The obtained bacterial solution is centrifuged and precipitated at a speed of 3000r / min for ...

Embodiment 2

[0022] Example 2 Toxicity test of Bacteroides fragilis CGMCC No.7280

[0023] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0024] (1) Acute toxicity test in mice

[0025] Sixty C57BR / C mice weighing 22±2 g (half male and half male) were divided into high-dose group, low-dose group and control group, 20 in each group. Each mouse in the high-dose group was intragastrically administered 10 12 cfu / ml bacterial solution 0.5ml, the low dose group was administered orally 10 times a day 9 cfu / ml bacterial solution 0.5ml, and the control group was fed with 0.5ml normal saline every day. Continuous observation for 14 days.

[0026] Experimental results: The mice grew normally, no death was observed, and the LD50 was undetectable.

[0027] (2) Rat toxicity test

[0028] Take 30 SD rats (half male and half female) of 150±20 g, and divide them into 3 groups: high and low dose group and control group. Each rat ...

Embodiment 3

[0033] Example 3 The therapeutic effect of Bacteroides fragilis CGMCC No.7280 on bacterial vaginosis

[0034] Animal model establishment: Escherichia coli (ATCC25922) and Staphylococcus aureus (ATCC25923) strains were recovered and passaged, and prepared with sterile saline to a concentration of 1.8*10 9 / ml of the bacterial solution, mixed in a ratio of 1:1 to form an infectious bacterial solution. First wash the mouse vagina with sterile PBS solution (pH8.5) for 3 times (every interval of 5 minutes), then apply sterile paraffin oil to the No. 5 scalp needle silicone tube, and slowly insert it into the mouse vagina for about 1 cm~1. cm, the injection volume of each bacteria is 0.025ml / 100g, 1 time / day. After the inoculation of bacteria, the vaginal lesions of the mice were observed daily, and vaginal secretions were stained for microscopic examination. When the mouse vagina was obviously congested, red and swollen, accompanied by a large amount of purulent secretions, a ...

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Abstract

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparation of a composition for treating bacterial vaginitis. The experiments show that bacteroides fragilis is safe and nontoxic and strong in pharmacologic action, and has good treating effect to treat bacterial vaginitis, thereby indicating that the bacteroides fragilis has good edible and medicinal prospects. According to the invention, a novel use of bacteroides fragilis is explored and a novel application field is developed. Bacteroides fragilis as a probiotic can be used for preparing foods or medical compositions for treating bacterial vaginitis.

Description

technical field [0001] The invention relates to the application technical field of Bacteroides fragilis, in particular to the application of Bacteroides fragilis in preparing a composition for treating bacterial vaginosis. Background technique [0002] Bacterial vaginosis is also called bacterial vaginosis. Bacterial vaginosis is a mixed infection caused by the imbalance of normal flora in the vagina. However, there were no inflammatory changes in the clinical and pathological features. It mostly occurs in sexually active women, and is a common and frequently-occurring disease in the majority of women. [0003] Under normal physiological conditions, there are various anaerobic and aerobic bacteria in the human vagina, 90% of which are Lactobacillus. Lactobacillus can decompose glycogen into lactic acid, and the hydrogen peroxide it produces has a certain decomposition effect, which can inhibit the growth of other pathogenic bacteria in the vagina and help form a good vagi...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P15/02A61P31/04A23L1/30A61K35/741
Inventor 智发朝白杨刘洋洋
Owner 广东知光生物科技有限公司
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