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Preparation method of modified zein peptide

A technology of zein and zein, which is applied in the field of zein modification, can solve the problems of complex double-enzyme hydrolysis production process, inability to reduce the number of disulfide bonds, and poor water solubility of modified proteins, and achieve favorable Environmental protection, easy promotion and application, low price effect

Inactive Publication Date: 2015-09-30
张俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the hydrolysis rate of using alkaline protease to directly hydrolyze unpretreated zein is low;
[0006] ③ Enzymatic hydrolysis of zein alkaline protein requires the use of two enzymes, papain and compound flavor enzymes, for double-enzyme hydrolysis. Facilitate industrialized mass production
The main disadvantage of this method is: since the zein in the raw corn gluten powder contains a large number of disulfide bonds, it leads to aggregation between molecules and is insoluble in water
Direct acylation of zein can not reduce the number of disulfide bonds contained in zein in protein powder, and the resulting modified protein has poor water solubility, which is not conducive to large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The concrete steps of a kind of preparation method of modified zein peptide are as follows:

[0033] (1) Preparation of zein emulsion

[0034] Using commercially available zein flakes as raw materials, the zein flakes are placed in a pulverizer to pulverize the zein flakes. Collect the pulverized material and sieve it with a 200-300-mesh sieve. Collect sieved powder and unsifted zein flakes respectively, and send the collected unsifted zein flakes into a pulverizer for crushing again; The mass (g) of soluble protein powder: the ratio of the volume (ml) of distilled water is the ratio of 1: 100, under the stirring velocity of 3000r / min, when zein protein powder is all dispersed in distilled water, just prepared Zein Emulsion.

[0035] (2) Carry out enzymatic hydrolysis

[0036] After step (1) is completed, first pump the zein emulsion prepared in step (1) into the reaction kettle, and feed steam into the interlayer of the reaction kettle, and heat up at a stirring sp...

Embodiment 2

[0047] A kind of preparation method of modified zein peptide is the same as embodiment 1, wherein:

[0048] In the (1) step, sieve with 200 mesh sieves, the mass (g) of the zein powder of sieve: the ratio configuration of the volume (mL) of distilled water is 1: 50, at 4000r / Stir at a stirring speed of 1 min until the zein powder is dispersed in distilled water.

[0049] In the (2) step, according to the ratio of alkaline protease quality (g): zein powder quality (g) in zein emulsion: mercaptoethanol volume (mL) is 1: 40: 15 ratio, First add mercaptoethanol, stir evenly, then add alkaline protease, and carry out stirring enzymatic hydrolysis for 80 minutes. The molecular weight cut-off of the ultrafiltration membrane is 5000Da, and the ultrafiltration pressure is 0.05Mpa. Ultrafiltration is carried out until the ultrafiltration pressure reaches 0.22Mpa. Stop ultrafiltration.

[0050] In the (3) step, according to enzymatic hydrolysis zein ultrafiltration classification liqu...

Embodiment 3

[0055] A kind of preparation method of modified zein peptide is the same as embodiment 1, wherein:

[0056] In the (1) step, sieve with 200 mesh sieves, the mass (g) of the zein powder of sieving: the ratio configuration of the volume (mL) of distilled water is 1: 80 ratio configuration, at 5000r / Stir at a stirring speed of 1 min until the zein powder is dispersed in distilled water.

[0057]In the (2) step, according to the ratio of alkaline protease quality (g): zein powder quality (g) in zein emulsion: mercaptoethanol volume (mL) is 1: 50: 12 ratio, First add mercaptoethanol, stir evenly, then add alkaline protease, and carry out stirring enzymatic hydrolysis for 70 minutes. The molecular weight cut-off of the ultrafiltration membrane is 4000Da, and the ultrafiltration pressure is 0.10Mpa. Ultrafiltration is carried out until the ultrafiltration pressure reaches 0.16Mpa. Stop ultrafiltration.

[0058] In the (3) step, according to enzymatic hydrolysis zein ultrafiltratio...

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Abstract

The invention relates to a preparation method of modified zein peptide and belongs to the technical field of utilization of zein. According to the preparation method of the modified zein peptide, market zein flakes serve as raw materials. The the preparation method of the modified zein peptide includes the following steps of preparing a zein emulsion, conducting single enzyme hydrolysis and an acylation reaction, drying, and obtaining the modified zein peptide. A plurality of byproducts are obtained through separation and concentration. The preparation method of the modified zein peptide has the advantages of being simple in technology, easy to operate, simple in production equipment, safe in production, high in comprehensive utilization efficiency of resources, free from discharge of waste gas, waste water and industrial residue, beneficial to environmental protection, low in production cost, convenient to popularize and apply, strong in water-solubility of the prepared product and the like. In addition, the preparation method of the modified zein peptide can be widely applied to modification production of the zein flakes. The product and the byproducts prepared through the preparation method of the modified zein peptide can be widely applied to industries such as textile, medicicine, food and chemistry.

Description

1. Technical field [0001] The invention belongs to the technical field of zein utilization, and in particular relates to a modification method of zein. 2. Background technology [0002] my country's annual corn output accounts for about 30% of the world's total output, ranking second in the world, and is the main raw material for the production of feed and corn starch. Zein flakes are the main by-products in the production of corn starch. For every ton of starch produced, about 0.15 tons of zein powder will be produced. The content of proline, alanine and leucine in zein is relatively high. Glamin has the characteristics of filmability, cohesiveness, gelation, etc., and also has the characteristics of enteric solubility and anti-oxidation. Therefore, it can be widely used in industries such as medicine, food and chemical industry. However, there are a large number of disulfide bonds in zein, so it can only be dissolved in 60-95% ethanol aqueous solution but not in pure wat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/34C07K1/107C07K4/10A23K1/16A61K8/65
Inventor 陆文立周小华冯琳颜李秀万玉萍
Owner 张俊
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