Tissue culture method of fargesii

Abstract

The invention relates to a tissue culture method of fargesii. The method comprises the following steps of: acquiring annual produced branches of a fargesii seed tree, and sequentially carrying out branches hydroponic sprouting, asepticizing, buds inducing and multiplying, adventitious bud rooting culturing, hardening-seedling, and transplanting to obtain fargesii tissue cultured seedling. After the tissue culture method is used, the specific, robust and plant diseases and insect pests-free fargesii female parent is selected, a large batch of high-quality fargesii tissue cultured seedling can be obtained within a short time through a specific tissue culture technique and a specific culture condition, so that the scientific research and production requirements can be met; compared with the other breeding methods, the tissue culture method needs less female parent materials, 1-2 stem(s) can be bred on a large scale, the good properties of the female parent can be preferably kept, the seedling is transplanted to outdoors, and the rate of survival can be more than 95%; the tissue culture method is quick in breeding speed, good in nursery stock quality, and high in emergence rate; and the stock and the cion do not need to be bred, so that the floor space can be greatly reduced, and the labor cost and the management cost can be reduced.

Description

technical field [0001] The invention relates to a method for tissue culture of woody plants, in particular to a method for tissue culture of ash catalpa. Background technique [0002] Catalpa fargesii (Catalpa fargesii) is a tall deciduous tree belonging to the Bignoniaceae genus Catalpa. Its distribution range is wide, mainly distributed in the central and western regions of my country, with the Weihe River, Jinghe River, Fenhe River Basin and Qinling Mountains as the concentrated areas, scattered around villages and in valleys. Ash catalpa has a well-developed root system, fast growth, strong wind resistance, strong soil fixation ability, cold and drought resistance, straight texture, tough and dense texture, and is a high-quality fast-growing timber tree species. [0003] Usually, ash catalpa is propagated by grafting and cuttings. Grafting requires cultivating the rootstock (catalys) 1 year in advance, and collecting 1-year-old branches or shoots from the stem of the f...

AI Technical Summary

Problems solved by technology

Grafting requires cultivating the rootstock (catalys) 1 year in advance, and collecting 1-year-old branches or shoots from the stem of the female parent as the scion in the following year; during the grafting process, the diameter of the scion is generally larger than that of the rootstock, resulting in poor joint healing and average survival rate At about 70%, the grafted seedlings have weak wind resistance, and the base of the rootstock sprouts more, so it needs to be wiped continuously, which increases the management cost

Cutting propagation needs to collect the current year’s branches, seedlings or roots to bury the dry (root) for germination, and collect semi-lignified shoots for cuttings. The requirements for the germination bed and cutting bed temperature, humidity and relative air humidity are relatively strict, and the management cost is high. The survival rate of cuttings is low, generally around 50%.

[0004] Tissue culture is an efficient way of asexual reproduction, with high reproduction efficiency and low cost. It is an effective means to solve the problem of rapid multiplication of elite clones of C.

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