Microorganism and application thereof

A technology of microorganisms and genes, applied in the field of biology, can solve problems that need to be deepened

Active Publication Date: 2013-07-10
WUHAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0005] However, the current research ...

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  • Microorganism and application thereof
  • Microorganism and application thereof
  • Microorganism and application thereof

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specific Embodiment

[0133] According to an embodiment of the present invention, the microorganism may further contain nucleic acid sequences encoding other genes, thereby endowing the microorganism with additional functions. According to an embodiment of the present invention, the microorganism further includes a second nucleic acid sequence, which can encode 2-ketoacid decarboxylase ARO10 Gene or its functional equivalent, alcohol dehydrogenase ADH2 Gene or its functional equivalent. According to a specific embodiment of the present invention, the second nucleic acid sequence can encode 2-ketoacid decarboxylase ARO10 Gene, 2-ketoacid decarboxylase ARO10 Gene expression translates to ARO10 enzyme, ARO10The enzyme can catalyze the decarboxylation of 2-keto acid, an intermediate product of amino acid metabolism, into aldehyde, and the second nucleic acid sequence can also encode alcohol dehydrogenase ADH2 Gene or its functional equivalent, alcohol dehydrogenase ADH2 Gene expression tr...

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Abstract

The invention provides a microorganism and an application thereof. The microorganism is used for preparing aliphatic ester. The microorganism comprises a first nucleotide sequence, a second nucleotide sequence, a third nucleotide sequence and a fourth nucleotide sequence, wherein the first nucleotide sequence is used for encoding acyltransferase AT genes or a functional equivalent thereof, and the second nucleotide sequence is used for encoding 2-keto acid decarboxylase ARO10 gene or a functional equivalent of the 2-keto acid decarboxylase ARO10 gene, ethanol dehydrogenase ADH2 genes or a functional equivalent of the ethanol dehydrogenase ADH2 gene and acyl coenzyme A reductase FAR genes or a functional equivalent of the acyl coenzyme A reductase FAR gene; the third nucleotide sequence is used for encoding endoglucanase gene or a functional equivalent of the endoglucanase gene and beta-glucosaccharase gene B-GL or a functional equivalent of the beta-glucosaccharase gene, and the fourth nucleotide sequence is used for encoding acyl-coenzyme A reductase FAR gene or a functional equivalent of the acyl-coenzyme A reductase FAR gene. By utilizing the microorganism, the preparation efficiency of the aliphatic ester can be effectively improved.

Description

[0001] technical field [0002] The invention relates to the field of biotechnology, and the invention relates to microorganisms and uses thereof. More specifically, the present invention relates to microorganisms, systems for conversion into organisms, methods for producing fatty acid esters, fatty acid ester products and systems for producing fatty acid esters. [0003] Background technique [0004] In order to alleviate the pressure of the energy crisis, countries are actively looking for renewable alternative energy sources. And has successively developed many renewable energy sources such as water energy, wind energy, tidal energy, solar energy and biomass energy. However, due to the limitations of the environment, climate, geographical factors, and huge initial development investment, water energy, wind energy, tidal energy, and solar energy can only be used in specific areas, and it is difficult to popularize them. Biomass energy is a general term for a class of cl...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N1/15C12N1/19C12N7/01C12P7/62C12M1/00
Inventor 刘天罡郭道义
Owner WUHAN UNIV
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