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Enterobacter gergoviae and its use in bioflocculation

A microbial flocculant, enterobacteriaceae technology, applied in the field of microorganisms, can solve the problem of no enterobacteriaceae and achieve good pH stability, short flocculation time and high efficiency

Inactive Publication Date: 2014-12-10
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening high-efficiency flocculant-producing bacteria is still an important research content in microbial flocculants, and there are no reports about Enterobacteriaceae as flocculation microorganisms

Method used

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  • Enterobacter gergoviae and its use in bioflocculation
  • Enterobacter gergoviae and its use in bioflocculation
  • Enterobacter gergoviae and its use in bioflocculation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0042] Implementation Case 1 Screening and identification of high-efficiency flocculation bacteria

[0043]The flocculation strains were screened from activated sludge and landfill leachate, and the isolated strains were respectively inserted into 250ml wide-mouthed Erlenmeyer flasks equipped with 50ml of seed culture medium (see Example 3 below), and placed in a shaker at 30 Cultivate at 200rpm for 24 hours, and take the culture solution to measure the flocculation rate. A strain of Enterobacter diazoae with the highest flocculation activity was obtained, its biological deposit number was CCTCC M2013042 strain, and it was named eth-2. and use it as the target for subsequent experiments.

[0044] Identification method: eth-2 such as figure 1 As shown, through 16S rRNA sequencing (the sequencing result is shown in SEQ ID NO: 1) and compared in NCBI, the similarity with Enterobacter.Gergoviae (AB682278.1) reached 99%.

Embodiment 2

[0045] The preparation of embodiment 2 culture medium

[0046] 1. Culture medium preparation

[0047] 1. Solid plate medium: tryptone, 10.0g; yeast powder, 5.0g; NaCl, 10.0g; agar powder, 20.0g; distilled water, 1L.

[0048] 2. Seed medium: glucose, 10.0g; K 2 HPO 4 , 5.0g; MgSO 4 ·7H 2 O, 0.2g; KH 2 PO 4 , 2.0g; NaCl, 0.1g; urea, 0.5g; yeast powder, 0.5g; distilled water 1L.

[0049] 3. Fermentation medium: glucose, 10.0g; K 2 HPO 4 , 5.0g; MgSO 4 ·7H 2 O, 0.2g; KH 2 PO 4 , 2.0g; NaCl, 0.1g; sodium nitrate, 1.0g; distilled water 1L.

[0050] Second, the culture and fermentation conditions of microorganisms:

[0051] The culture conditions of the solid plate medium: the eth-2 strain was inoculated in the solid medium and cultured at 30°C for 1 day, and then stored in a refrigerator at 4°C after being matured.

[0052] The cultivation conditions of the seed medium: the seed medium was sterilized at 115°C for 20 minutes, and then inserted into the strains of the s...

Embodiment example 3e

[0056] Implementation case 3eth-2 as preparation of microbial flocculant

[0057] 1. Centrifuge a certain volume of culture medium at 8000rpm and 5minl to collect the supernatant and precipitate respectively, and wash the precipitate with distilled water for 3 times and then resuspend it in a considerable volume of distilled water. Compare the supernatant, the precipitate suspension and the fermentation broth respectively The flocculation effect on kaolin to determine the distribution of flocculation activity. The result is as figure 2 As shown, the flocculation rates of the fermentation broth, bacterial suspension and supernatant were 90.45%, 94.36% and 63.20%, respectively, indicating that the flocculation activity of eth-2 was mainly distributed in the bacterial sediment, so the following experiments were used After repeated washing with distilled water for 3 times, the bacterial pellet resuspended in distilled water was used as a microbial flocculant.

[0058] 2. The ef...

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Abstract

The invention belongs to the field of the microbial technology, relates to a method for preparing a biological flocculant by enterobacter gergoviae and enterobacter gergoviae use, and especially relates to enterobacter gergoviae and its use in preparation of the biological flocculant. The preparation method of the biological flocculant comprises the following steps of inoculating a sterile strain medium with enterobacter gergoviae, carrying out culture, collecting the culture solution, inoculating a fermentation medium with the culture solution, carrying out culture at a temperature of 30 DEG C for 18h, carrying out fermentation broth centrifugal separation, and removing a supernatant and precipitates to obtain the biological flocculant. The preparation method has simple processes and a low cost. The biological flocculant has the advantages of high activity, strong heat resistance, good pH tolerance and no secondary pollution, can improve sludge settlement in sewage treatment, and has a flocculation rate of 90% and a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for preparing a biological flocculant by enterobacteria and an application thereof. Background technique [0002] my country is one of the countries with serious water shortage and pollution. In order to solve the problem of water shortage, in addition to saving water, strengthening the treatment of sewage is an urgent problem to be solved at present. Among water treatment methods, flocculation is one of the most commonly used methods. Domestic sewage and various industrial wastewater often contain different types and quantities of suspensions and colloids. By adding flocculants in water treatment, these sols and suspensions are destabilized, and then condensed into large particles and quickly precipitated. [0003] There are many types of flocculants. Inorganic flocculants such as polyaluminum chloride and polyferric chloride are reliable in operation, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F1/54C12R1/01
Inventor 宋立岩唐薇乔婧黄亦存尹雅洁李斗
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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