Dehydrating and embedding improvement method of human body or animal tissues

A technology of animal tissue and dehydration bag, which is applied in the preparation of test samples, etc., can solve the problem of fragility of paraffin tissue blocks, and achieve the effect of less toxic and side effects, smooth tissue and high safety

Inactive Publication Date: 2013-08-07
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide an improved method of dehydration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1:

[0019] In a method for improving dehydration and embedding of human or animal tissues of the present invention, skin tissue (can also be loose tissues such as glands and mucous membranes) is selected as the implementation object, and the specific operation steps are as follows:

[0020] 1. Fixation: Obtain skin tissue samples through clinical surgery or animal experiments, and quickly cut them into blocks of about 0.5cm×1cm×1cm (thickness×length×width), first use 4% paraformaldehyde (mass / volume ratio, solvent For PBS) fixation, overnight at 4℃, time is 8h-16h, and then placed in 0.02% (mass / volume ratio) NaN 3 Stored in the PBS storage solution for later use, can be stored for a long time.

[0021] 2. Dehydration: First, the fixed skin tissue sample is dehydrated by ethanol concentration gradient: the skin tissue sample is dehydrated by 50% volume concentration of ethanol for 20 minutes, 70% volume concentration of ethanol for 1 hour, and 80% volume concentration...

Example Embodiment

[0025] Example 2:

[0026] An improved method for dehydration and embedding of human or animal tissues of the present invention selects liver tissue (also can be substantive normal tissues such as spleen, kidney, muscle, etc., or cancer tissue) as the implementation object. The specific operation steps are as follows :

[0027] 1. Fixation: Obtain liver tissue samples through clinical surgery or animal experiments, and quickly cut them into blocks of about 0.5cm×1cm×1cm, fix them with 4% paraformaldehyde, overnight at 4℃, for 8h-16h, then Put in 0.02%NaN 3 Stored in the PBS storage solution for later use, can be stored for a long time.

[0028] 2. Dehydration: First, the fixed liver tissue samples are dehydrated by ethanol concentration gradient: the liver tissue samples are dehydrated by 50% volume concentration of ethanol for 30 minutes, 70% volume concentration of ethanol for 1 hour to 2 hours, and 80% volume concentration of ethanol. Dehydrate for 1h~2h, dehydrated with 95% vo...

Example Embodiment

[0032] Example 3:

[0033] In a method for improving dehydration and embedding of human or animal tissues of the present invention, bone tissue is selected as the implementation object, and the specific operation steps are as follows:

[0034] 1. Fixation: Obtain bone tissue samples through clinical surgery or animal experiments, quickly cut them into blocks of about 0.5cm×1cm×1cm, fix them with 4% paraformaldehyde, overnight at 4℃, for 8h-16h, then Put in 0.02%NaN 3 Stored in the PBS storage solution for later use, can be stored for a long time.

[0035] 2. Dehydration: First, the fixed bone tissue samples are dehydrated by ethanol concentration gradient: the bone tissue samples are dehydrated by 50% volume concentration ethanol for 30 minutes, 70% volume concentration ethanol for 2 hours, and 80% volume concentration ethanol for 1 hour. , 95% volume concentration ethanol dehydration for 1 hour, again with 95% volume concentration ethanol dehydration for 1 hour, dehydrated ethanol...

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PUM

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Abstract

The invention discloses a dehydrating and embedding improvement method of human body or animal tissues. The method comprises the following steps of: fixing; dehydrating; transparence treatment; wax-dipping and embedding. The method comprises the following steps of: first, dehydrating fixed tissue by using ethanol with different concentrations from low concentration to high concentration in a gradient manner; then, placing the tissue dehydrated in the gradient manner into a mixed solution of absolute ethyl alcohol and butanol in a volume ratio of 1:1-1:5 for dehydrating, wherein the time for dehydrating by using the mixed liquid is 30-60 minutes, and then, further using butanol to dehydrate the tissue which is dehydrated by using the mixed liquid at 4-16 DEG C for 8-20 hours; at the end of dehydration, dipping the tissue in butanol for transparence treatment; and finally, wax-dipping and embedding the transparent tissue. The method provided by the invention is simple and convenient to operate, high in safety and reliability, remarkable in effect and wide in application.

Description

technical field [0001] The invention relates to an improved method for dehydration and embedding of human or animal tissues, and the method can be widely used in research in the fields of medicine, biology and the like. Background technique [0002] Paraffin section technology is the main experimental method of medical histology, developmental biology and other research, and it is also an important means to observe the structural changes of human or animal tissues. The morphological observation, immunohistochemistry, in situ hybridization and in situ PCR detection techniques of human, animal and plant specimens are all inseparable from specimen tissue processing and paraffin section making. [0003] The ultimate purpose of making paraffin sections is to dehydrate and embed the tissue into paraffin blocks. Conventional paraffin sectioning techniques include five basic steps, namely fixation, dehydration, clearing, paraffin immersion, and embedding. Among them, the dehydrati...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 周军周丽温蒲圆
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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