Dehydrating and embedding improvement method of human body or animal tissues
A technology of animal tissue and dehydration bag, which is applied in the preparation of test samples, etc., can solve the problem of fragility of paraffin tissue blocks, and achieve the effect of less toxic and side effects, smooth tissue and high safety
Inactive Publication Date: 2013-08-07
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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The invention discloses a dehydrating and embedding improvement method of human body or animal tissues. The method comprises the following steps of: fixing; dehydrating; transparence treatment; wax-dipping and embedding. The method comprises the following steps of: first, dehydrating fixed tissue by using ethanol with different concentrations from low concentration to high concentration in a gradient manner; then, placing the tissue dehydrated in the gradient manner into a mixed solution of absolute ethyl alcohol and butanol in a volume ratio of 1:1-1:5 for dehydrating, wherein the time for dehydrating by using the mixed liquid is 30-60 minutes, and then, further using butanol to dehydrate the tissue which is dehydrated by using the mixed liquid at 4-16 DEG C for 8-20 hours; at the end of dehydration, dipping the tissue in butanol for transparence treatment; and finally, wax-dipping and embedding the transparent tissue. The method provided by the invention is simple and convenient to operate, high in safety and reliability, remarkable in effect and wide in application.
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