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Dehydrating and embedding improvement method of human body or animal tissues

A technology of animal tissue and dehydration bag, which is applied in the preparation of test samples, etc., can solve the problem of fragility of paraffin tissue blocks, and achieve the effect of less toxic and side effects, smooth tissue and high safety

Inactive Publication Date: 2013-08-07
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide an improved method of dehydration embedding that is simple, effective, low in cost, overcomes the fragility of paraffin tissue blocks when slicing, and is not easy to damage human or animal tissues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An improved method for dehydration and embedding of human or animal tissue according to the present invention selects skin tissue (it can also be loose tissue such as glands and mucous membranes) as the implementation object, and the specific operation steps are as follows:

[0020] 1. Fixation: Obtain skin tissue samples through clinical operations or animal experiments, quickly cut them into pieces of about 0.5cm×1cm×1cm (thickness×length×width), and first use 4% paraformaldehyde (mass / volume ratio, solvent fixed in PBS), overnight at 4°C for 8h to 16h, and then placed in 0.02% (mass / volume ratio) NaN 3 Store in the PBS storage solution for later use, and can be stored for a long time.

[0021] 2. Dehydration: First, dehydrate the fixed skin tissue samples with ethanol concentration gradient: dehydrate the skin tissue samples with 50% volume concentration of ethanol for 20 minutes, 70% volume concentration of ethanol for 1 hour, and 80% volume concentration of alcohol...

Embodiment 2

[0026] An improved method for dehydration and embedding of human or animal tissue according to the present invention. Liver tissue (which can also be substantial normal tissue such as spleen, kidney, muscle, etc., or cancer tissue) is selected as the implementation object, and the specific operation steps are as follows :

[0027] 1. Fixation: Obtain liver tissue samples through clinical operations or animal experiments, quickly cut them into pieces with a size of about 0.5cm×1cm×1cm, first fix them with 4% paraformaldehyde, and overnight at 4°C for 8h to 16h, then Put in 0.02%NaN 3 Store in the PBS storage solution for later use, and can be stored for a long time.

[0028] 2. Dehydration: First, dehydrate the fixed liver tissue samples with ethanol concentration gradient: dehydrate the liver tissue samples through 50% volume concentration of ethanol for 30 minutes, 70% volume concentration of ethanol dehydration for 1h~2h, and 80% volume concentration of ethanol. Dehydratio...

Embodiment 3

[0033] An improved method for dehydration and embedding of human or animal tissue of the present invention, bone tissue is selected as the implementation object, and the specific operation steps are as follows:

[0034] 1. Fixation: Obtain bone tissue samples through clinical operations or animal experiments, quickly cut them into pieces about 0.5cm×1cm×1cm in size, first fix them with 4% paraformaldehyde, put them overnight at 4°C for 8h-16h, then Put in 0.02%NaN 3 Store in the PBS storage solution for later use, and can be stored for a long time.

[0035] 2. Dehydration: First, dehydrate the fixed bone tissue samples with an ethanol concentration gradient: dehydrate the bone tissue samples with 50% volume concentration of ethanol for 30 minutes, 70% volume concentration of ethanol for 2 hours, and 80% volume concentration of ethanol for 1 hour. , 95% volume concentration of ethanol dehydration for 1 hour, again dehydration with 95% volume concentration of ethanol for 1 hour...

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PUM

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Abstract

The invention discloses a dehydrating and embedding improvement method of human body or animal tissues. The method comprises the following steps of: fixing; dehydrating; transparence treatment; wax-dipping and embedding. The method comprises the following steps of: first, dehydrating fixed tissue by using ethanol with different concentrations from low concentration to high concentration in a gradient manner; then, placing the tissue dehydrated in the gradient manner into a mixed solution of absolute ethyl alcohol and butanol in a volume ratio of 1:1-1:5 for dehydrating, wherein the time for dehydrating by using the mixed liquid is 30-60 minutes, and then, further using butanol to dehydrate the tissue which is dehydrated by using the mixed liquid at 4-16 DEG C for 8-20 hours; at the end of dehydration, dipping the tissue in butanol for transparence treatment; and finally, wax-dipping and embedding the transparent tissue. The method provided by the invention is simple and convenient to operate, high in safety and reliability, remarkable in effect and wide in application.

Description

technical field [0001] The invention relates to an improved method for dehydration and embedding of human or animal tissues, and the method can be widely used in research in the fields of medicine, biology and the like. Background technique [0002] Paraffin section technology is the main experimental method of medical histology, developmental biology and other research, and it is also an important means to observe the structural changes of human or animal tissues. The morphological observation, immunohistochemistry, in situ hybridization and in situ PCR detection techniques of human, animal and plant specimens are all inseparable from specimen tissue processing and paraffin section making. [0003] The ultimate purpose of making paraffin sections is to dehydrate and embed the tissue into paraffin blocks. Conventional paraffin sectioning techniques include five basic steps, namely fixation, dehydration, clearing, paraffin immersion, and embedding. Among them, the dehydrati...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 周军周丽温蒲圆
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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