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Method for quickly determining tannin content in mulberry leaves

A technique for rapid determination of medium tannins, applied in the preparation of test samples, measurement of color/spectral properties, etc., which can solve the problems of many repetitive processes, low extraction efficiency, and long measurement time.

Inactive Publication Date: 2013-08-14
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main defect of this method is that the measurement time is long, repeated processes are many, the extraction efficiency is low, and the energy consumption is high; the solvent extraction method is to utilize polar organic solvents such as methanol and ethanol to measure after the tannin is dissolved. 50% ethanol was extracted for 3 hours, and the tannin content in the tuber was determined to be 0.93%.
The main disadvantage of this method is that the extraction time is long, and there are many impurities in the eluate, which have a great influence on the accuracy of the measurement data.
So far, there is no research report on the method of rapid determination of tannin content in mulberry leaves

Method used

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  • Method for quickly determining tannin content in mulberry leaves
  • Method for quickly determining tannin content in mulberry leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of reagent

[0021] (1) Folir-Denis reagent: Weigh 100g of sodium tungstate and 20g of phosphomolybdic acid into a 1000mL flask, add 750mL of water to dissolve, and then add 50mL of phosphoric acid. Connect the condensing device, heat and reflux on the boiling water bath for 2h, cool to room temperature, add water to make up to 1000mL to obtain the Folir-Denis reagent, and store it in a brown bottle for later use.

[0022] (2) Tannin standard solution: Weigh 0.0100g of tannin acid, add a small amount of water to dissolve it, transfer it to a 100ml volumetric flask, add water to mix well and set the volume to 100ml to obtain a tannin standard solution, save it for later use, and the concentration of tannin is 0.1 mg / mL.

[0023] (3) Saturated Na 2 CO 3 Solution: weigh 240g anhydrous Na 2 CO 3 , add 1200mL of water, stir and dissolve evenly in a water bath at 50°C, let stand for 24h, filter, and the obtained filtrate is saturated Na 2 C...

Embodiment 2

[0024] Embodiment 2: the drafting of tannin standard curve

[0025] Take 6 50mL volumetric flasks, add 30mL distilled water respectively, then draw 0.0mL, 1.0mL, 2.0mL, 3.0mL, 4.0mL, 5.0mL of tannin standard solution into the volumetric flasks, add 2.5mL of Folir-Denis reagent and saturate Sodium carbonate solution 5.0mL, add water to make up to 50mL (corresponding to the concentration of tannin is 0.0μg / mL, 2.0μg / mL, 4.0μg / mL, 6.0μg / mL, 8.0g / mL, 10.0μg / mL), Mix well, let it stand for 30 minutes, adjust to zero with a blank, measure the absorbance at 750nm, and obtain a standard curve (such as figure 1 shown).

Embodiment 3

[0026] Embodiment 3: the mensuration of tannin in the fresh mulberry leaf:

[0027] (1) Drying of mulberry leaves: Wash the fresh mulberry leaves, dry them in an oven at 80°C, crush them into mulberry leaf powder, and store them in sealed bags at room temperature for later use.

[0028] (2) Ultrasonic extraction of tannins in mulberry leaves: Take 0.5g of mulberry leaf powder in a 100mL glass bottle with a blue cap, add 12.5mL of ethanol solution with a mass ratio concentration of 65%, cover the bottle cap and put it in an ultrasonic cleaner. Extract at 120W ultrasonic power and 65°C for 15 minutes; take out the glass bottle with blue cap, filter, and save the filtrate for later use; add 12.5mL of ethanol solution with a mass ratio concentration of 65% to the residue and repeat the ultrasonic extraction under the same conditions to obtain the filtrate, and repeat the extraction in this way 3 times, the filtrates were combined and the volume was adjusted to 50mL, and the extrac...

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Abstract

The invention relates to a method for quickly determining tannin content in mulberry leaves, which comprises the following steps: drawing a tannin standard curve, extracting tannin in mulberry leaves by ultrasonic, determining the tannin content in the mulberry leaves, and the like. Under the auxiliary action of ultrasonic, the 65% ethanol solution used as a solvent can be utilize to extract tannin in mulberry leaves in a short time (at most 1 hour), thereby greatly shortening the extraction time; and a plurality of or even tens of mulberry leaf samples can be simultaneously determined according to the specification of the ultrasonic cleaner, thereby enhancing the work efficiency.

Description

technical field [0001] The invention relates to the technical field of plant component analysis, in particular to a method for quickly measuring the tannin content in mulberry leaves. Background technique [0002] Tannin, also known as tannin, is a polyphenol composed of glucose (and its polyol) ester of gallic acid (or its polymer), a polymer of cycloalkanol and its derivatives, and a mixture of the two. It is found in plants widely exist. Tannins are divided into two categories: hydrolyzable and condensed (proanthocyanidins). In the past 10 years, oligomeric proanthocyanidins have been widely used in the fields of health care products and cosmetics in Europe, the United States, Japan and other countries, and enjoy the reputation of "skin vitamins". . Tannin is widely used in the tanning industry for tanning bottom leather, industrial leather, luggage leather, etc. Tannin is also an important medicinal component of Chinese herbal medicines such as Baibu and Geranium. ...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N21/31
Inventor 黄先智丁晓雯韩小丹
Owner SOUTHWEST UNIVERSITY
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