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Tremella fuciformis strain ion beam injection mutation breeding method and Tremella fuciformis strain bred therethrough

A technology of ion beam implantation and white fungus strains, which is applied in botany equipment and methods, fungal products, plant gene improvement, etc., can solve problems that have not been reported yet, and achieve the effects of stable genetic traits, widening the range, and increasing yield

Active Publication Date: 2013-09-04
北京市辐射中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Haven't seen any reports about using ion beam implantation technology to induce and breed tremella

Method used

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  • Tremella fuciformis strain ion beam injection mutation breeding method and Tremella fuciformis strain bred therethrough
  • Tremella fuciformis strain ion beam injection mutation breeding method and Tremella fuciformis strain bred therethrough

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Determination of ion implantation dose

[0035] Starting strain: Tremella fuciformis BRC010 preserved in the Microbial Breeding Laboratory of Beijing Radiation Center. The Tremella fuciformis BRC010 was isolated from wild Tremella collected in Fujian. Co., Ltd. has carried out cultivation experiment verification and has preserved the strains.

[0036] Obtaining Tremella Basidiospores

[0037] In the laboratory, the starting strain of the white fungus is bottle-planted using a sawdust medium, and the ears are cultivated at 20-22° C. to obtain fresh white fungus fruiting bodies.

[0038] Take 20-30g of fresh and clean Tremella fungus slices (fruiting bodies), remove them with tweezers and a scalpel, put them into a 50ml sterile centrifuge tube filled with 35ml of sterile water, cover them well, and place them on a vortex shaker vigorously Vibrate several times, or hold the two ends of the centrifuge tube with your hands, shake vigorously up and down for sev...

Embodiment 2

[0047] The screening of embodiment 2 white fungus bacterial strains

[0048] Cultivation of white fungus strains

[0049] The basidiospores that have germinated on the white fungus PDA flat plate of counting survival number among the embodiment 1 are picked respectively on the new PDA flat board, and each flat plate is inoculated with 1 basidiospores of germination, and the white fungus basidiospores after germination is exactly Tremella fungus, will Tremella fungus is cultured in a constant temperature incubator at 20-25°C, and white hyphae will form around the fungus, and some will form yeast-like spores.

[0050] Selection of Tremella strains

[0051] Tremella fungus forms round or nearly round colonies after germinating hyphae, and measuring the diameter of the colony can preliminarily judge the growth speed of Tremella fungus, and select the tremella fungus with a faster growth rate.

[0052] Liquid Culture of Tremella Fungus Strains

[0053] The selected tremella colo...

Embodiment 3

[0059] Example 3 A Tremella fungus strain screened out by the method of the present invention

[0060] A Tremella fungus strain screened out by the method of the present invention (see Examples 1 and 2 for specific methods) was subjected to a passaging stability test for 15 passages, and each time it was paired with C. ratio, the fruiting body yield was stable, and there was no significant difference in the yield between generations (P>0.05), and Tremella fungus had no change in flower size and gloss. The obtained high-yielding strain, the yield increased by 13.5% compared with the original strain, the tremella polysaccharide content increased by 5.41%, and the genetic traits were stable. The tremella strain was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on April 24, 2013, address : Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, 100101. ...

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Abstract

The invention relates to a Tremella fuciformis strain ion beam injection mutation breeding method and a Tremella fuciformis strain bred therethrough. The breeding method comprises the following steps: 1, washing fresh auricles of an original Tremella fuciformis strain for obtaining basidiospores on the fresh auricles, coating the basidiospores onto a metal culture dish, and carrying out aseptic air blow drying; 2, carrying out low-energy N<+> ion beam injection mutation of the Tremella fuciformis basidiospores on the metal culture dish under a vacuum condition, wherein the radiation induced mutation dosage is 5*10<14>-5*10<15>ions / cm<2>; 3, culturing the mutated Tremella fuciformis basidiospores, and screening Tremella fuciformis strains with the thalli having high dry weights; and 4, pairing the screened strains with a Hypoxylon sp. strain, cultivating, and screening a Tremella fuciformis strain having a high output. The invention also provides the Tremella fuciformis strain obtained by breeding through the method. The Tremella fuciformis strain provided by the invention has a preservation number of CGMCC No.7458, the output of the Tremella fuciformis strain is 13.5% higher than that of the original strain, and the Tremella fuciformis polysaccharide content of the Tremella fuciformis strain is 5.41% higher than that of the original strain.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a tremella strain ion beam implantation mutagenesis breeding method and a cultivated tremella strain. Background technique [0002] Tremella, also known as white fungus, belongs to the kingdom of fungi, Basidiomycota, class Tremella, order Tremella, Tremellaceae, and genus Tremella. my country is the main producer of white fungus, and its export volume accounts for more than 90% of the world's white fungus trade volume. At present, the existing cultivars of Tremella mainly include yellow Tremella and white Tremella, which mainly come from the long-term domestication and artificial selection of wild Tremella species. The yield of Tremella species cultivated by this traditional Tremella species breeding method is not high. And it is easy to degrade and unstable. At present, there is an urgent need to explore high-yield, stable, and high-quality tremella strain...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01G1/04A01H15/00
Inventor 刘桂君尚宏忠顾海科吴美钦李庭伟周思静孟佑婷杨素玲温贤芳
Owner 北京市辐射中心