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Sedative and hypnotic active saponin components of spina date seed, as well as separation and purification method and application of sedative and hypnotic active saponin components

A technology for separation and purification of jujube seed, which is applied in the field of saponin components of soothe the nerves and hypnotic activity and its separation and purification.

Inactive Publication Date: 2013-09-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some differences among the research results. For example, Guo Shengmin et al. (Chinese Medicinal Materials. 1998, (21) 11: 578-579; Northwest Medical Journal. 1996, (8) 11: 166-168) reported that the total flavonoids, All saponins have hypnotic activity. Woo.Siekwoo et al. (Phytochemistry.1978, 18:353~355) also believe that jujuboside A is one of the active ingredients, but Wu Shuxun et al. (Chinese Journal of Traditional Chinese Medicine. 1991, 16(7): 435 ~437) The study found that jujube seed saponin A has no central nervous inhibitory effect, but can strengthen the excitatory effect of amphetamine on the contrary. It is believed that jujube seed saponin A may not be the active ingredient for calming the nerves; Yuan Changlu et al. (Chinese Medicine Bulletin. 1987, 12(3): 34~35) Through experiments, it is pointed out that flavonoids are also one of the effective ingredients of sedation and hypnosis, while fatty oil extracted from petroleum ether has no such effect, but Zhao Qiuxian et al. The effect of jujube seed fatty oil on the central nervous system was studied, and it was believed that jujube seed oil may also be an active ingredient for sedation and hypnosis

Method used

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  • Sedative and hypnotic active saponin components of spina date seed, as well as separation and purification method and application of sedative and hypnotic active saponin components
  • Sedative and hypnotic active saponin components of spina date seed, as well as separation and purification method and application of sedative and hypnotic active saponin components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of saponins of soothing hypnotic active saponin from Jujube seed

[0038] A sample of wild jujube seed powder (after frying and crushing, the wild jujube seed is passed through a 40-mesh sieve, and dried at 60°C for 24h), placed in a Soxhlet extractor with 10 times the volume of petroleum ether and defatted at 60°C for 4 hours to obtain residue and wild jujube seed oil; The residue was taken out, dried, crushed, and passed through a 40-mesh sieve to obtain defatted jujube seed powder. The defatted jujube seed powder was extracted with 25 times the volume of 70% ethanol in a 90°C water bath for 3 hours, and filtered to obtain residue I and alcohol. Liquid; evaporate the alcohol liquid to a paste under reduced pressure at 60°C, add water to dissolve it, filter and remove impurities to obtain an aqueous solution, add 1 volume of water-saturated n-butanol to the aqueous solution to extract, discard the water layer, and obtain n-butyl Alcohol layer I; alka...

Embodiment 2

[0039] Example 2 Separation and Purification of Saponins from Jujube Seed Soothing Hypnotic Activity

[0040] Due to the large polarity of saponin, low-activity alumina or silica gel is generally used as the adsorbent, and then solvents of different polarity are used for elution according to different saponin. Dry 100-200 mesh silica gel for column chromatography at 110°C for 1 hour, then rinse with distilled water to remove impurities; then soak in 95% ethanol for 24 hours; after vacuum degassing, wet it into chromatography Column (36mm×80mm). Column packing method: first add a certain amount of water to the column, then pour the silica gel with water into the column, discharge excess water through the column, keep the water surface above the silica gel layer by more than 3cm, until the silica gel layer is 7cm from the top of the column~ 8cm, spare. Load the saponin sample solution with a concentration of 25mg / ml, a sample volume of 10ml, 60% ethanol solution as the eluent, ad...

Embodiment 3

[0043] Example 3 Experimental determination of hypnotic activity

[0044] (1) Influence on the autonomous and coordinated movement of mice

[0045] (1) Autonomous movement

[0046] Suanzaoren soothing hypnotic active saponins components Ⅰ and Ⅱ were mixed into 10mg / ml medicinal solution. Thirty mice, half male and half male, were randomly divided into three groups with 10 mice in each group. Each group was given 0.15ml / 10g·d of liquid medicine (equivalent to 17g / kg·d of crude drug, about 50 times the clinical adult dose). The blank group was given the same volume of normal saline once a day for 3 consecutive days. Fasting for 24h before the last dose. 45 minutes after the last administration, the mice were placed in a cardboard box and allowed to adapt for 5 minutes, and the number of activities within 5 minutes was recorded (measure the walking time of the mice and the number of times the forelimbs were raised). Compared with the blank group, there is a very significant differen...

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Abstract

The invention discloses sedative and hypnotic active saponin components of a spina date seed, as well as separation and purification method and application of the sedative and hypnotic active saponin components, and belongs to the field of separation of natural products. The invention designs a new and relatively safe extracting route without introducing obviously toxic solvents and agents. The separation and purification method is characterized by comprising the following steps of: extracting a spina date seed sedative and hypnotic active saponin sample from a spina date seed sample by the alcohol extraction method and n-butanol extraction method; and then separating spina date seed sedative and hypnotic active saponin components I and II from the saponin sample by the column chromatography and thin-layer chromatography. The two spina date seed saponin components separated from the spina date seed show obvious hypnotic activity; and the natural sedative and hypnotic active components extracted and separated from traditional Chinese medicine can be used for solving the problems of multiple side effects and addiction of chemical synthetic drugs and is beneficial to human health and contributed to all the society.

Description

Technical field [0001] The invention belongs to the field of natural product separation, and particularly relates to the soothing hypnotic active saponin component of Jujube seed and its separation and purification method and application. Background technique [0002] With the development of society and the acceleration of the pace of life, more fear and anxiety of failure lurks in people's lives. Competition and fight for the game have brought tremendous pressure to people's spirits, and the incidence of insomnia and anxiety has risen sharply. Insomnia is the most common type of sleep disorder, which has great harm to human function, intelligence and immunity. Long-term insomnia not only damages people's thinking activities, but also affects people's immune system and ultimately shortens lives. In the clinic, chemical synthetic drugs with sedative and hypnotic effects are commonly used to treat insomnia. The survey found that about 44% of patients take benzodiazepines (BDZ) a...

Claims

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Application Information

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IPC IPC(8): A61K36/725A61P25/20
Inventor 姜建国张恬恬陈健许喜林朱良
Owner SOUTH CHINA UNIV OF TECH
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