Application of inonotus obliquus polysaccharide used as feed additive for litopenaeus vannamei
A technology of Inonotus obliquus and shrimp feed, which is applied in the application, animal feed, animal feed and other directions, can solve the problem of inability to improve immunity, and achieve the effect of promoting sustainable development, increasing weight gain rate, and broadening the scope of application
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Embodiment 1
[0037] Weigh 100 kg of Litopenaeus vannamei basal feed (commercially available, Hessant Litopenaeus vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 50 g of Inonotus obliquus polysaccharide (commercially available, Zhejiang Fangge Pharmaceutical Co., Ltd. Co., Ltd.), after mixing evenly, use a SLX-80 twin-screw extruder to make pellet feed with a diameter of 1.0 mm, and dry it at 55 °C.
Embodiment 2
[0039] Weigh 100kg of Litopenaeus vannamei basal feed (commercially available, Hessente vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 150g of Inonotus obliquus polysaccharide (commercially available, Zhejiang Fangge Pharmaceutical Co., Ltd. Co., Ltd.), after mixing evenly, use a SLX-80 twin-screw extruder to make pellet feed with a diameter of 1.0 mm, and dry it at 55 °C.
Embodiment 3
[0041] Weigh 100kg of basic feed for Litopenaeus vannamei (commercially available, Hessian Litopenaeus vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 80g of Inonotus obliquus polysaccharide, mix well and use SLX-80 The feed pellets with a diameter of 1.0 mm were made by a twin-screw extruder and dried at 55 °C.
[0042] The preparation method of Inonotus obliquus polysaccharide is as follows:
[0043] (1) Strain activation: use Inonotus obliquus (commercially available, purchased from Xuzhou Normal University) as the strain, inoculate it on the slant strain medium at 27°C, and cultivate it for 250 hours to obtain the activated strain; The formulation of the seed medium is: 40 g / 100mL of malt extract, 4 g / 100mL of peptone, 10 g / 100mL of agar, and the pH value is 5.4-5.6.
[0044] (2) Liquid strain culture: transfer the activated strain into the liquid seed medium at 27°C, cultivate on a shaker for 4.5 days until the logarithmic phase...
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