Application of inonotus obliquus polysaccharide used as feed additive for litopenaeus vannamei

A technology of Inonotus obliquus and shrimp feed, which is applied in the application, animal feed, animal feed and other directions, can solve the problem of inability to improve immunity, and achieve the effect of promoting sustainable development, increasing weight gain rate, and broadening the scope of application

Inactive Publication Date: 2014-07-16
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  • Abstract
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AI Technical Summary

Problems solved by technology

Although the prawn diet can meet the nutritional requirements of Litopenaeus vannamei for growth, it cannot improve its immunity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 100 kg of Litopenaeus vannamei basal feed (commercially available, Hessant Litopenaeus vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 50 g of Inonotus obliquus polysaccharide (commercially available, Zhejiang Fangge Pharmaceutical Co., Ltd. Co., Ltd.), after mixing evenly, use a SLX-80 twin-screw extruder to make pellet feed with a diameter of 1.0 mm, and dry it at 55 °C.

Embodiment 2

[0039] Weigh 100kg of Litopenaeus vannamei basal feed (commercially available, Hessente vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 150g of Inonotus obliquus polysaccharide (commercially available, Zhejiang Fangge Pharmaceutical Co., Ltd. Co., Ltd.), after mixing evenly, use a SLX-80 twin-screw extruder to make pellet feed with a diameter of 1.0 mm, and dry it at 55 °C.

Embodiment 3

[0041] Weigh 100kg of basic feed for Litopenaeus vannamei (commercially available, Hessian Litopenaeus vannamei compound feed produced by Qingdao Changsheng Zhongke Aquatic Feed Co., Ltd.), add 80g of Inonotus obliquus polysaccharide, mix well and use SLX-80 The feed pellets with a diameter of 1.0 mm were made by a twin-screw extruder and dried at 55 °C.

[0042] The preparation method of Inonotus obliquus polysaccharide is as follows:

[0043] (1) Strain activation: use Inonotus obliquus (commercially available, purchased from Xuzhou Normal University) as the strain, inoculate it on the slant strain medium at 27°C, and cultivate it for 250 hours to obtain the activated strain; The formulation of the seed medium is: 40 g / 100mL of malt extract, 4 g / 100mL of peptone, 10 g / 100mL of agar, and the pH value is 5.4-5.6.

[0044] (2) Liquid strain culture: transfer the activated strain into the liquid seed medium at 27°C, cultivate on a shaker for 4.5 days until the logarithmic phase...

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Abstract

The invention discloses the application of an Inonotus obliquus polysaccharide as a feed additive for Litopenaeus vannamei. The amount of the Inonotus obliquus polysaccharide added to the basic feed of Litopenaeus vannamei is 500-1500 mg / kg of the basic feed of Litopenaeus vannamei. In the invention, the Inonotus obliquus polysaccharide is used as an additive in the feed of Litopenaeus vannamei, which can significantly increase the weight gain rate and specific growth rate of Litopenaeus vannamei, significantly reduce the feed coefficient, and at the same time significantly increase the serum lysozyme, Alkaline phosphatase and total antioxidant capacity, increase serum nitric oxide synthase, superoxide dismutase, reduce serum malondialdehyde content, its effect is equivalent to β-glucan, is a new type of immune enhancer, It has broad application prospects in the field of Litopenaeus vannamei culture.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to the application of an Inonotus obliquus polysaccharide as a feed additive for Litopenaeus vannamei. Background technique [0002] Litopenaeus vannamei, commonly known as Penaeus vannamei, is one of the three largest shrimp species in the world with the highest production of farmed shrimp. Because of its fast growth rate, low nutritional requirements, large shrimp meat content, delicious meat, and rich nutrition, it is favored by consumers. In recent years, with the rapid development of large-scale and intensive farming of Litopenaeus vannamei, the outbreak of breeding diseases has become more and more frequent. In order to control the occurrence of diseases, chemical drugs and antibiotics have been used in large quantities, resulting in drug residues, resistance Problems such as drug properties and environmental pollution seriously affect the health of consumers. Therefore, it is par...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/18A23K1/16
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