Swine immunopotentiator and its preparation method
An immune enhancer and extract technology, applied in the field of pig immune enhancer and its preparation, can solve the problems of increased harm of immunosuppressive diseases, increased resistance of pathogenic bacteria to antibiotics, decreased quality of meat products, frequent occurrence of diseases, etc.
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[0023] Preparation of phytase-producing Bacillus subtilis bacterial powder:
[0024] 1. Obtaining of fermented liquid: Obtain phytase-producing Bacillus subtilis fermented liquid by step-by-step expansion cultivation of slanted strains;
[0025] (1) First-level seed culture: insert the preserved phytase-producing Bacillus subtilis slant strain into a 500-milliliter shake flask, with a medium loading capacity of 100 milliliters, a rotary shaker at 160 rpm, and a culture temperature of 30 ℃, the incubation time is 24 hours;
[0026] (2) secondary seed cultivation: the primary seed is inserted into a 500 milliliter secondary seed shake flask according to the inoculum size of 10%, and the cultivation condition is the same as that of the primary seed;
[0027] (3) Tertiary seed cultivation: insert the secondary seeds with 10% inoculum size into 5000 milliliters of tertiary seed shaking flasks, with 1000 milliliters of culture medium, 120 rpm on a rotary shaker, and the culture is ...
Embodiment 1
[0036] An immune enhancer for pigs and a preparation method thereof, comprising the following raw materials in parts by weight: an immune enhancer for pigs and a preparation method thereof, consisting of the following raw materials in parts by weight: 12 parts of yeast β-glucan, poplar flower extract 10 parts of bamboo shoot shell extract, 15 parts of phytase-producing Bacillus subtilis powder, 6 parts of propolis, 2 parts of γ-aminobutyric acid, 1 part of mannan oligosaccharide, and 3 parts of chlorogenic acid.
[0037] The preparation method of the poplar flower extract is as follows:
[0038] Take dried poplar flowers and grind them through 40 mesh, put them in a container and add 5 times the amount of water, control the temperature at 90°C for 1 hour, filter the filtrate, add ethanol to 45% for precipitation, and concentrate the obtained supernatant in vacuo Then freeze-dry to obtain poplar flower extract.
[0039] The preparation method of the bamboo shoot husk extract: ta...
Embodiment 2
[0043] An immune enhancer for pigs and a preparation method thereof, comprising the following raw materials in parts by weight: an immune enhancer for pigs and a preparation method thereof, consisting of the following raw materials in parts by weight: 18 parts of yeast β-glucan, poplar flower extract 15 parts, 18 parts of bamboo shoot shell extract, 20 parts of phytase-producing Bacillus subtilis powder, 8 parts of propolis, 3 parts of γ-aminobutyric acid, 2.5 parts of mannan oligosaccharides, and 4 parts of chlorogenic acid.
[0044] The preparation method of the poplar flower extract is as follows:
[0045] Take dried poplar flowers and grind them through 40 mesh, put them in a container and add 6 times the amount of water, keep the temperature at 95°C for 1.25h, filter to get the filtrate, then add ethanol to 45% concentration for precipitation, the obtained supernatant, vacuum Concentrated and then freeze-dried to obtain poplar flower extract.
[0046] The preparation met...
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