Extraction method of E-viniferin

A technology of vinifolin and extraction method, which is applied in the field of viniferin extraction, and can solve problems such as research lag and low viniferin

Inactive Publication Date: 2013-10-02
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely low content of vinifenin in plants, there are relatively few research reports on products with high content of vinifenin, so the research on it, especially the research on biological activity, is relatively lagging behind

Method used

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  • Extraction method of E-viniferin
  • Extraction method of E-viniferin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] HPLC detection method of viniferin content:

[0030] Chromatographic column: C-18 250×4.6mm

[0031] Detection wavelength: 310nm

[0032] Flow rate: 1.0ml / min

[0033] Column temperature: 25°C

[0034] time (min) 0 5 15 18 29 35 57 57.1 61 61.1 65 65 B ratio (%) 20 30 30 37 37 50 50 100 100 20 20 stop

[0035] Mobile phase: A: 1% glacial acetic acid aqueous solution;

[0036] B: 1% methanol solution of glacial acetic acid.

Embodiment 2

[0038] (1) Extraction: add 2000g of water to 500g of grape seeds and soak at 95℃ under normal pressure for 5 times. Combine and let stand until the temperature is lower than 50°C, discard the precipitate, and obtain the supernatant;

[0039] (2) Adsorption and separation of macroporous resin: the extract from (1) is adsorbed by HP20 macroporous resin, and then gradiently eluted with water and ethanol aqueous solution with a concentration of 30%, 50%, 70%, and 95% by volume. / 2 column retention volume as a receiving volume, through thin-layer chromatography combined with analytical HPLC detection, collect components rich in vinifenin, combine them, at a temperature ≤ 60 ° C, a vacuum of 0.06 ~ 0.08 Concentrated under reduced pressure at MPa, the sugar content is 40Brix, the enrichment of Vinifenin;

[0040] (3) Separation of polyamide resin: the enrichment of vinifenin obtained after adsorption and separation of macroporous resin is dispersed into water (1:4) for separation of...

Embodiment 3

[0043] Steps (1) ~ (3) are the same as in Example 3;

[0044] (4) Toyopearl HW-40 column chromatography separation: Toyopearl HW-40 column chromatography was carried out on the components rich in vinifenin after separation by polyamide, and the volume percentage of water was 50%, 60%, 70%, 80% % methanol aqueous solution gradient elution, each 1 / 4 column retention volume is used as a receiving volume, through thin layer chromatography and other methods combined with analytical HPLC detection, with the pure product of vernifenin as the reference substance, collected rich in vinifenin Components, and concentrated under reduced pressure at a temperature of ≤60°C and a vacuum of 0.06-0.08MPa, with a sugar content of 10Brix, determined by the method of Example 1: the content of vinifenin was 81.9%.

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Abstract

The invention discloses an extraction method of E-viniferin, which comprises the following steps: (1) by using roots, stems or leaves of plants of pinaceae, rosaceous and the like as a raw material, adding an ethanol water solution, leaching, filtering, and concentrating the supernatant under reduced pressure to obtain an extract with the sugar degree of 2-40; (2) dispersing the extract in water, adsorbing by a macroporous resin, carrying out gradient elution with an ethanol water solution, detecting by thin-layer chromatography and analytic HPLC (high performance liquid chromatography), collecting an E-viniferin-rich component, and concentrating under reduced pressure to obtain a concentrate with the sugar degree of 10-40; and (3) carrying out polyamide resin separation on the concentrate, carrying out gradient elution with an ethanol water solution, detecting by thin-layer chromatography and analytic HPLC, collecting the E-viniferin-rich component, and concentrating the eluate under reduced pressure to obtain the extract of which the sugar degree is 10-20 and the E-viniferin content is 20-50 wt%. The method disclosed by the invention can obtain the high-content E-viniferin extract.

Description

technical field [0001] The invention relates to a method for extracting vinifenin. Background technique [0002] E-viniferin is a dimer of resveratrol, which is its metabolite in plants. Resveratrol (resveratrol) is a kind of phytoalexin produced by some plants when they are infected by pathogenic bacteria or the environment deteriorates. Immunity and other characteristics [1-2] . [0003] In recent years, studies have found that the degree of plant disease resistance is closely related to the metabolites of resveratrol in plants. Therefore, the study of resveratrol metabolites has become a new hotspot in the research of stilbene secondary metabolites. Current studies have shown that Viniferin has various effects such as antibacterial, anti-inflammatory, anti-HIV, and whitening [3-4] . However, due to the extremely low content of vinifenin in plants, there are relatively few research reports on products with high content of vinifenin, so the research on it, especially t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/80
Inventor 刘岱琳刘丹孙华庚
Owner 天津市尖峰天然产物研究开发有限公司
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