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Method for preparing microbial preparation of bacillus sp and application thereof

A technology of Bacillus subtilis and microbial inoculum, applied in the field of microorganisms, can solve the problems of not easy to colonize in plants, not easy to manipulate, unfavorable for popularization and application, etc., and achieves the effects of easy storage and transportation, growth inhibition, and convenient operation.

Inactive Publication Date: 2013-10-16
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

First of all, the currently developed biocontrol resources are easily affected by external conditions, are not easy to colonize in plants, and the control effect is unstable
Secondly, the fermentation liquid of bio-control bacteria is not easy to store and transport. In the process of use, the preparation of the fermentation liquid is cumbersome and difficult to manipulate, which is not conducive to further popularization and application.

Method used

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  • Method for preparing microbial preparation of bacillus sp and application thereof
  • Method for preparing microbial preparation of bacillus sp and application thereof
  • Method for preparing microbial preparation of bacillus sp and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Fermentation of Bacillus sp. EDR4

[0027] Activate the Bacillus sp. strain stored at -80°C with the preservation number CGMCC No.2133 on LB solid medium, pick a single colony of Bacillus sp. EDR4, and inoculate it in a 100 mL LB culture medium (250mL Erlenmeyer flask), shake culture at 28°C, 150rpm for 18-24h, then inoculate 100mL of LB culture liquid with 1% seed solution, shake culture at 28°C, 150rpm for 48h, and obtain the fermentation product of the bacteria one. Among them, the LB culture solution is composed of 10g peptone, 10g yeast powder, 5gNaCl, 1000mL distilled water, and the pH value is 7.2.

[0028] Inoculate corn flour solid medium with 1% seed liquid, and culture at 28° C. for 48 hours to obtain the fermentation product 2 of the bacteria. Among them, the corn flour medium contains 50g of corn flour, 35g of soybean flour, 40g of bran, 1000mL of distilled water, and a natural pH value. Cornmeal culture is based on 121°C, sterilized for 30 minutes, and ...

Embodiment 2

[0030] Preparation method of microbial preparation of Bacillus sp. EDR4 active ingredient with preservation number CGMCC No.2133

[0031] Add 1% sodium carboxymethyl cellulose to the two fermentation products in Example 1 (LB fermented liquid, soybean flour medium), then mix with diatomaceous earth of equal weight to make two kinds of powders respectively Bacteria A, B, no caking. Store in airtight condition at room temperature.

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Abstract

The invention discloses a bacterial strain of bacillus sp EDR4, the preservation number is CGMCC No. 2133. According to the invention, the bacterial strain with preservation number of CGMCC No. 2133 is used as an active ingredient to prepare the microbial preparation; the microbial preparation has a simple preparation technology, convenient operation, a short incubation period, easily accessible raw materials, and stable product quality, has no phytotoxicity on crops, is environment-friendly, and has a remarkable effect in preventing and curing sclerotinia rot of colza; therefore, the bacterial strain of bacillus sp EDR4 with the preservation number of CGMCC No. 2133 provided by the invention can be applied to the prevention and curing of sclerotinia rot of colza.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bacillus subtilis, a preparation method of the microbial preparation thereof and its application to the prevention and treatment of rape sclerotinia. Background technique [0002] Sclerotinia sclerotiorum is a fungal disease caused by Sclerotinia sclerotiorum (Lib.) de Bary, which occurs in all major rape producing areas in the world. In the main rape producing areas in my country, rape sclerotinia, as a frequent disease, ranks first among the three major diseases of rape (downy mildew, virus disease), the incidence rate is generally 10-30%, and it can reach 80% in severe years. % or more, the yield will be reduced by 11% to 73%, and the oil content will be reduced by 1% to 5%, which will greatly reduce the quality and yield of rapeseed. Because Sclerotinia sclerotiorum likes a humid and warm environment, it is particularly prominent in major rape producing areas such as...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/12A01N63/00A01P3/00C12R1/125
Inventor 韩青梅陈亚菲高小宁黄丽丽秦虎强
Owner NORTHWEST A & F UNIV
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