Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting citrulline from selenium-contained watermelon plant tissues

A plant tissue, citrulline technology, applied in chemical instruments and methods, preparation of organic compounds, preparation of urea derivatives, etc., can solve the problems of high energy consumption, increased load, low product purity, etc., to achieve comprehensive utilization, The effect of reducing stress and low overall energy consumption

Active Publication Date: 2013-10-23
苏州奕方华知识产权运营有限公司
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Patent ZL200510072130 "A method of extracting citrulline from watermelon rind" discloses pressing through concentration, cation exchange, and crystallization. This method has no purification process and the product has low purity
Application No. 200710120714 "An industrial method for extracting natural L-citrulline from plant tissues such as watermelon or pumpkin", this method adopts methods such as membrane separation, cation exchange, crystallization, recrystallization, etc., but this method crystallization and recrystallization The process adopts ethanol crystallization method, which only removes metal ions, and other amino acids still remain in it, so the product purity is not high
Application No. 201010156824 "A method of purifying citrulline from watermelon", which adopts a 3-6 times acidic water ultrasonic extraction process, which increases the load of subsequent concentration, high energy consumption, high product cost, and unreasonable process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Peel the watermelon, crush and remove the seeds, wash the stems and leaves, crush and squeeze the juice to obtain 1,000 liters of mixed juice, treat the mixed solution with pectinase at 40-50°C for 30-60 minutes, and then filter it with a 0.2 micron ceramic membrane cross-flow , to obtain 960 liters of clarified watermelon juice.

[0027] 2) Vacuum concentration of clarified watermelon juice to 480 liters;

[0028] 3) The resulting concentrated solution was adjusted to a pH value of 10.76 with concentrated ammonia water, cooled to 1° C., inserted a small amount of arginine seed crystals, and allowed to stand overnight. The crystals were collected, separated and purified to obtain arginine; 450 liters of supernatant was obtained, At 20-25°C, exchange and adsorb with D201 anion exchange resin at a flow rate of 1.0BV / h. After adsorption, wash the exchange column with 500 liters of pure water, collect the washing liquid, and distill the washing liquid under reduced press...

Embodiment 3

[0042] 1) Peel the watermelon, crush and remove the seeds, wash the stems and leaves, crush and squeeze the juice to obtain 1,000 liters of mixed juice, treat the mixed solution with pectinase at 40-50°C for 30-60 minutes, and then filter it with a 0.2 micron ceramic membrane cross-flow , to obtain 960 liters of clarified watermelon juice.

[0043] 2) Vacuum concentration of clarified watermelon juice to 100 liters;

[0044] 3) The resulting concentrated solution was adjusted to a pH value of 10.76 with concentrated ammonia water, cooled to 1° C., inserted with a small amount of arginine seed crystals, and left to stand overnight. The crystals were collected, separated and purified to obtain arginine; 95 liters of supernatant was obtained, At 20-25°C, exchange and adsorb with D201 anion exchange resin at a flow rate of 1.0BV / h. After adsorption, wash the exchange column with 100 liters of pure water, collect the washing liquid, and distill the washing liquid under reduced pres...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting citrulline from selenium-contained watermelon plant tissues. Citrulline, and partial glutamic acid and arginine are separated out through the steps of juicing, enzyme treatment clarification, concentration, anion exchange resin adsorption, ammonia water elution, crystallization and recrystallization. The yield of the citrulline product is 0.3%; the product is high in purity and low in energy consumption. The method provided by the invention has the advantages that resin washing water, ammonia water and the like are recycled, the process is low in pollution on environment and comprehensive utilization of watermelons and tissues thereof is realized.

Description

Technical areas: [0001] The invention belongs to the field of agricultural product processing, and specifically relates to a method for extracting and purifying citrulline from selenium gourd plant tissue. technical background: [0002] Watermelon, which belongs to the Cucurbitaceae family, is known by various names such as natural white tiger soup, cold melon, summer melon, and water melon. Its shape and skin color also include: oval or round, green, dark green, white or green with snake patterns, etc. Its pulp is light red, rich red, white or yellow, and is sweet and juicy. Watermelon is very nutritious and has extremely high nutritional value: in addition to not containing fat and cholesterol, it is rich in glucose, malic acid, fructose, protein amino acids, lycopene and vitamins A, B1, B2, C and other substances. It has the effects of clearing away heat, relieving summer heat, diuresis, and lowering blood pressure. It has certain auxiliary effects on nephritis, oliguri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C275/16C07C273/18C07C279/14C07C277/08C07C229/24C07C227/40
Inventor 周学义
Owner 苏州奕方华知识产权运营有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com