Safety evaluation method for analyzing fungus strain producing no mycotoxin based on whole genome sequence

A technology for safety evaluation and mycotoxins, which is applied in the fields of biochemical equipment and methods, and microbial determination/inspection.

Inactive Publication Date: 2013-10-23
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the traditional instrument detection and analysis is relatively sensitive, there are still shortcomings in the detection of mycotoxins such as multi-index analysis and the inability to identify undiscovered or diff

Method used

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  • Safety evaluation method for analyzing fungus strain producing no mycotoxin based on whole genome sequence
  • Safety evaluation method for analyzing fungus strain producing no mycotoxin based on whole genome sequence

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Embodiment 1

[0017] Embodiment 1: filamentous fungus Rhizopus sinensis ( Rhizopus chinensis ) Safety evaluation of whether CCTCC M 201021 produces mycotoxin:

[0018] Rhizopus chinensis Rhizopus chinensis It is an important functional microorganism in my country's traditional brewing industry. Rhizopus sinensis strain CCTCC M 201021 is a filamentous fungus isolated from Chinese liquor brewing Daqu (Xu Y, Wang D, Mu XQ, et al.. Biosynthesis of ethyl esters of short-chain fatty acids using whole- cell lipase from Rhizopus chinensis CCTCC M201021 in non-aqueous phase. J. Mol. Catal. B Enzym. 2002, 18:29 –37), according to the whole genome sequence data of the strain (GenBank number ANKS00000000), it was analyzed and judged whether Rhizopus sinensis has the ability to produce mycotoxins. potentially harmful. Focus on the main metabolic pathways and related genes of rhizopus, microsporin and typical filamentous mycotoxins, including PKS, NRPS and PKS-NRPS mixed metabolic pathways, terpen...

Embodiment 2

[0021] Embodiment 2: Aspergillus oryzae ( Aspergillus oryzae ) Safety evaluation of whether RIB326 produces mycotoxin:

[0022]The filamentous fungus Aspergillus oryzae ( Aspergillus oryzae ) RIB326 is a production strain isolated in the Japanese traditional brewing industry (Umemura, M, Koike, H, Yamane, N, et al. Comparative Genome Analysis Between Aspergillus oryzae Strains Reveals Close Relationship Between Sites of Mutation Localization and Regions of Highly Divergent Genes among Aspergillus Species. DNA RESEARCH, 2012, 19, 375–382). According to Aspergillus oryzae ( Aspergillus oryzae ) RIB326 whole genome sequence data (GenBank number BAEZ00000000), analyze and judge whether the strain has the potential hazard of producing mycotoxin. Focus on the possible mycotoxins derived from Aspergillus, such as the main pathways and related genes of typical mycotoxin metabolism such as aflatoxin and cyclopiazonic acid. Genome analysis showed that about half of the aflatox...

Embodiment 3

[0025] Embodiment 3: Aspergillus niger ( Aspergillus niger CBS 513.88) safety evaluation of mycotoxin production:

[0026] Aspergillus niger ( Aspergillus niger ) is an important fermentation industry production bacteria. In the whole genome data of Aspergillus niger strain CBS 513.88 (GenBank number GCA_000002855.1), after analysis, it was found that this strain has a relatively complete PKS pathway and a complete gene cluster of fumonisin synthesis pathway, and has the ability to produce this toxin, indicating that There is a potential safety risk of producing mycotoxins when the strain is used in industries such as food and medicine. In industrial production applications, the detection of the fermentation products of Aspergillus niger strains found that there are indeed mycotoxins such as fumonisins and ochratoxins. The production of fumonisin by fermentation of Aspergillus niger strain CBS 513.88 has been confirmed by literature reports (Frisvad JC, Larsen TO, Thrane ...

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Abstract

The invention relates to a safety evaluation method for analyzing a fungus strain producing no mycotoxin, based on a whole genome sequence. The method belongs to the fields of bio-fermentation technology and food safety. According to the invention, with a bioinformatics method, mycotoxin synthesis and metabolism pathways and key genes are analyzed, wherein PKS, NRPS and PKS-NRPS hybrid metabolic pathways, terpenoid compound metabolic pathways and other metabolic pathways are analyzed. Based on sequenced fungal whole genome data, whether the fungus has the latent mycotoxin producing capacity is analyzed, and the safety of the fungus in food industries in the aspects of fermented products and microbial strains is evaluated. As a recult, rhizopus chinensis CCTCCM201021 basically has no key gene or synthesis capacity for producing currently known mycotoxin, and a fermented product thereof is relatively safe. However, some strains of aspergillus species such as aspergillusniger have part of mycotoxin synthesis key gene clusters, and strain safety needs further analysis and determination. The method provided by the invention has important guiding and application values.

Description

technical field [0001] The invention relates to a method for evaluating the safety of fungal strains that do not produce mycotoxins based on whole genome sequence analysis, and belongs to the fields of microbial fermentation technology and food safety. Background technique [0002] Filamentous fungi are an important class of industrial microorganisms, and their fermentation products are widely used in many fields such as food, medicine, agriculture, energy, and chemical industry. In traditional food and modern industrial fermentation, as production strains, the safety of filamentous fungi is getting more and more attention. Especially in the food fermentation industry, some fungi have the ability to synthesize mycotoxins, which has a huge impact on human health. Hidden danger. [0003] More than 400 kinds of mycotoxins have been reported, most of which are mainly caused by Aspergillus ( Aspergillus ), ergot ( Cla Viceps ), Fusarium ( Fusarium ), Penicillium ( Penicil...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/02
Inventor 王栋徐岩吴荣
Owner JIANGNAN UNIV
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