Method for inhibiting the growth of Alternaria alternata and the accumulation of mycotoxins in Alternaria alternata

A technology of Alternaria sp. and mycotoxins is applied in the preservation of food ingredients as antimicrobials, food ingredients containing natural extracts, preservation of fruits and vegetables, etc., achieving good application prospects and market value, strong bacteriostatic properties, and stable performance. Effect

Pending Publication Date: 2018-09-18
XIANGTAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, the inhibition of cinnamaldehyde on postharvest Alternaria vi

Method used

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  • Method for inhibiting the growth of Alternaria alternata and the accumulation of mycotoxins in Alternaria alternata
  • Method for inhibiting the growth of Alternaria alternata and the accumulation of mycotoxins in Alternaria alternata
  • Method for inhibiting the growth of Alternaria alternata and the accumulation of mycotoxins in Alternaria alternata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Effect of cinnamaldehyde on Alternaria mycelium growth

[0046] 1. The effect of direct contact with cinnamaldehyde on the growth of Alternaria

[0047] (1) Prepare potato dextrose broth (PDB), divide PDB medium into 150 mL Erlenmeyer flasks, 100 mL each, then add Tween-80 to the Erlenmeyer flasks to make the concentration 0.5%, At 121 °C, autoclave for 20 min;

[0048] (2) After the medium is sterilized in step (1), cool it down to 40-50 °C, add cinnamaldehyde to make the final concentrations 0.00, 0.0125, 0.025, 0.050, 0.100 and 0.200 μL / mL , shake well;

[0049] (3) Use a 6 mm round hole puncher to make a lawn in the culture dish of Alternaria for 5 days, take 2 pieces of lawn and inoculate them into the Erlenmeyer flask in the step (2), and store them at 25 °C , shaking culture under the condition of 150 rpm;

[0050] (4) After culturing for 96 h, the Alternaria hyphae in the step (3) were taken out, rinsed with sterile water for 3 times, filtered with...

Embodiment 2

[0056] Example 2 Degradation experiment of cinnamaldehyde to Alternaria mycotoxins AOH and AME

[0057] 1. Preparation of mycotoxin AOH standard curve

[0058] (1) Dissolve 1.0 mg AOH toxin standard substance in acetonitrile to prepare a standard stock solution with a concentration of 0.5 mg / mL. Take 0.2 mL of the stock solution and dilute to volume with methanol in a 10 mL volumetric flask to obtain AOH toxin standard solutions with a concentration of 10 μg / mL. The prepared standard solutions were stored in a -20°C refrigerator;

[0059] (2) According to the needs, prepare the standard use solution of AOH standard solution with the concentration of 0.10, 0.20, 0.50, 1.00, 5.00 and 10.00 μg / mL; Store in -20 °C refrigerator.

[0060] 2. Mycotoxin AME standard curve preparation

[0061] (1) Dissolve 1.0 mg AME toxin standard substance in acetonitrile to prepare a standard stock solution with a concentration of 0.5 mg / mL. Take 0.4 mL of the stock solution and dilute to volum...

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Abstract

The invention discloses a method for inhibiting the growth of Alternaria alternata and the accumulation of mycotoxins in Alternaria alternata. The method is characterized in that a bacteriostatic agent used in the method is natural cinnamaldehyde essential oil which has a significant inhibitory effect on the Alternaria alternaria, with the minimum inhibitory concentration (MIC) being 0.2 MuL/mL. The mycotoxins are Alternariol (AOH) and Alternariol monomethyl ether (AME). After treatment with the cinnamaldehyde essential oil under MIC and 10 x MIC, toxin contents of the AOH and the AME in the Alternaria alternata decrease significantly. Therefore, the cinnamaldehyde essential oil, as a safe and pollution-free natural preservative, can not only control pathogenic bacterium Alternaria alternata after grapes are harvested, but also degrade the mycotoxins in the Alternaria alternata. The method has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of inhibiting pathogenic bacteria and toxin degradation in biological control, and in particular relates to a method for inhibiting the growth of Alternaria and the accumulation of mycotoxins in the body. Background technique [0002] Grape fresh fruit is sweet and juicy, nutritious and deeply favored by people. In 2014, China's annual output of table grapes reached 9 million tons, ranking first in the world. The grape skin is thin, the flesh is soft, and the water content is high, so it is easy to be infected by germs and rot. This brings difficulties to the storage, transportation, and prolongation of sales time of table grapes, resulting in huge economic losses; [0003] The pathogenic fungi of grape postharvest rot were mainly six genera: Botrytis cinerea, Aspergillus niger, Penicillium, Rhizopus, Alternaria and Phomopsis. Alternaria ( Alternaria alternata ) is one of the dominant postharvest pathoge...

Claims

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Application Information

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IPC IPC(8): A23B7/154A23L5/20
CPCA23B7/154A23L5/27A23V2002/00A23V2200/10A23V2250/21
Inventor 敬国兴许灵春陶能国杨文蒿吴育藩刘淼
Owner XIANGTAN UNIV
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