PCR (Polymerase Chain Reaction) device based on low-melting-point metal liquid drop and implantation method of PCR device

Abstract

The invention provides a PCR (Polymerase Chain Reaction) device based on a low-melting-point metal liquid drop and an implantation method of the PCR device. The PCR device is characterized in that serving as a micro-resistor, the metal liquid drop generates joule heat by electrifying so as to provide heat for PCR, and the metal liquid drop can be cooled through an active cooling manner or the natural convection of air around the metal liquid drop, the temperature of the metal liquid drop can be directly measured by adopting a micro-thermocouple or indirectly measured by measuring the resistance of the metal liquid drop, a PCR reagent liquid drop sample is adhered to the interior of a miniature container under the action of surface tension, the miniature container is buckled down in the center of the metal liquid drop so as to ensure that the reaction reagent liquid drop is in the metal liquid drop all the time, the joule electric current of the metal liquid drop is adjusted by a temperature control module, and the cyclic change of RCR temperature is completed by a cooling device. The low-melting-point metal liquid drop can serve as a PCR reaction carrier, so that the RCR time can be greatly shortened, and the reagent sample is effectively prevented from being lost due to evaporation in the PCR process.

Description

technical field [0001] The invention relates to a PCR method, in particular to a method using a low-melting-point metal droplet as a PCR reaction carrier and a heater. Background technique [0002] Polymerase chain reaction (PCR) is a technology for amplifying DNA gene fragments in vitro. This technology consists of three steps: high temperature denaturation, low temperature annealing and medium temperature extension. Increment rapidly. PCR technology can be widely used in disease diagnosis, biochemical analysis, forensic identification and other fields. Conventional microfluidic methods for realizing PCR technology include microchamber polymerase chain reaction and continuous flow polymerase chain reaction. [0003] Microcavity polymerase chain reaction is to process microcavities or microcavity arrays on the surface of silicon wafers and other substrates by micromachining methods as PCR reagent sample reactors. Measures to achieve PCR cycle, to achieve DNA gene fragment...

AI Technical Summary

Problems solved by technology

On the one hand, the temperature control system often heats and cools from a certain direction, and the reagent samples are often not completely evenly heated

When performing PCR temperature control on a microfluidic chip, reagent samples are often stored in chip materials with poor heat transfer properties and large heat capacity such as PDMS or glass, so the temperature cannot be controlled quickly and accurately

This will undoubtedly increase power consumption, reduce the diffusion reaction speed of reagent samples, reduce the temperature response speed of the system, and increase the PCR cycle time.

On the other hand, when the PCR reagent sample is used for PCR, diffusion and evaporation loss will occur during the reaction process, and the utilization rate of the reagent sample will decrease.

Images