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Reversibly assembled and decomposable fluorescent magnetic nano material and nano biological probe and preparation methods thereof

A nanobiological probe, magnetic nanotechnology, applied in the fields of chemistry, materials science, and biology, can solve the problems of non-destructive separation and in vitro culture of circulating tumor cells reported in few studies, and achieve excellent magnetic responsiveness, reversible capture and release, good repeatability

Inactive Publication Date: 2013-11-20
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on circulating tumor cells focuses on the detection of the number of circulating tumor cells in the blood, and there are few reports on the non-destructive isolation and in vitro culture of circulating tumor cells.

Method used

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  • Reversibly assembled and decomposable fluorescent magnetic nano material and nano biological probe and preparation methods thereof
  • Reversibly assembled and decomposable fluorescent magnetic nano material and nano biological probe and preparation methods thereof
  • Reversibly assembled and decomposable fluorescent magnetic nano material and nano biological probe and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Use the embedding method (refer to the method described in ZL200310111290.9) to 10nm γ-Fe 2 o 3 Embedded in acrylamide-styrene polymer nanospheres to prepare magnetic nanomaterials, the average particle size is 200nm, dispersed in ultrapure water to obtain acrylamide-styrene polymer magnetic nanomaterials with a concentration of 10mg / mL , and succinic anhydride: put 5 mL of 10 mg / mL acrylamide-styrene polymer magnetic nanomaterials in a small beaker, gradually add 0.1 g of succinic anhydride within 10 min, and use 1 M NaOH Adjust the pH value of the reaction to keep the pH value of the reaction system at about 4. After the addition of succinic anhydride, continue the reaction for 3 hours. Afterwards, absorb the succinic anhydride-formed acrylamide-styrene polymer magnetic nanomaterials with a magnetic stand, remove excess succinic anhydride, add ultrapure water to wash several times, and disperse the final product in ultrapure water to obtain Carboxy-terminated ma...

Embodiment 2

[0064] (1) Use the method of self-assembly (refer to the method described in ZL201010266616.5) to combine 10 nm γ-Fe 2 o 3 Magnetic nanomaterials were prepared by assembling multilayers on the surface of succinic anhydrided acrylamide-styrene polymer nanospheres, with an average particle size of 400 nm, and dispersed in ultrapure water to obtain magnetic nanomaterials with a concentration of 10 mg / mL.

[0065] (2) Take 1.0mL 10mg / mL magnetic nanomaterials, add 1mL 10mg / mL PEI (750kDa) aqueous solution and incubate at room temperature for 10h, then centrifuge at 16000rpm for 20min to remove excess PEI to obtain PEI-coated magnetic nanomaterials.

[0066] (3) Add 1mL 3×10 to the PEI-coated magnetic nanomaterial -5 M thioglycolic acid-modified water-soluble CdTe quantum dots were incubated at room temperature for 30 minutes, centrifuged at 16,000 rpm for 10 minutes, and the centrifuged product was dispersed in 1 mL of ultrapure water to prepare CdTe-coated magnetic nanomaterials...

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Abstract

The invention discloses a reversibly assembled and decomposable fluorescent magnetic nano material and a nano biological probe and preparation methods thereof. A fluorescent magnetic nano material is prepared through incubation of a magnetic nano material and a cationic polymer and then assembly of a water-soluble quantum dot onto the surface thereof, then a sodium alginate modified fluorescent magnetic nano material is prepared through incubation of sodium alginate and the quantum-dot-coated fluorescent magnetic nano material, then multi layers of sodium alginate are assembled onto the surface of the fluorescent magnetic nano material through layer upon layer self-assembly through utilization of bridging effects of a cross-linking agent, and then the reversibly assembled and decomposable fluorescent magnetic nano material can be obtained. The reversibly assembled and decomposable nano biological probe can be obtained by incubation of the fluorescent magnetic nano material activated by the cross-linking agent and a biomolecule-sodium alginate conjugate. The nano biological probe can realize capture and release of biological target molecules through the cross-linking agent and ethylene diamine tetraacetic acid (EDTA). The preparation methods of the fluorescent magnetic nano material and the nano biological probe are simple and practicable, and have strong operability and good repeatability.

Description

[0001] technical field [0002] The invention belongs to the fields of biology, chemistry and material science, and relates to a reversibly assembled and decomposed fluorescent magnetic nanometer material, a nanobiological probe and a preparation method thereof. Background technique [0003] Fluorescent magnetic nanomaterials have functions such as magnetic controllability and fluorescent tracking, and the specific functionalized molecules modified on their surface can be used in the detection of tumor markers, DNA analysis, identification, sorting and imaging of tumor cells and other fields. However, the current fluorescent magnetic nanobiological probes can only be used to specifically recognize targets, and cannot achieve reversible capture and release of targets, thus limiting their applications in the biomedical field. [0004] As a natural polysaccharide, sodium alginate has the characteristics of stability, solubility, viscosity and safety. Sodium alginate hydrogel h...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/88H01F1/36H01F1/11B82Y30/00B82Y40/00C07K17/00G01N33/68
Inventor 黄卫华谢敏陆宁宁庞代文程世博
Owner WUHAN UNIV
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