Active site of mulberry twig, as well as preparation method and application thereof

A technology of active parts and mulberry branches, which is applied in the field of preparation of active parts of traditional Chinese medicine, can solve the problems of insufficient content determination and quality control of active parts of mulberry branches for hypoglycemia, and achieve industrialization cost saving, good repeatability and high safety. Effect

Inactive Publication Date: 2013-12-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above application does not give a complete concept and exact data of the active part of Mori twig for lowering blood sugar

Method used

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  • Active site of mulberry twig, as well as preparation method and application thereof
  • Active site of mulberry twig, as well as preparation method and application thereof
  • Active site of mulberry twig, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 The preparation method of the active part of Morus twig

[0037] Get mulberry branch 100g, 4 parts, pulverize, sieve. Boil with pure water, 25% ethanol water, 50% ethanol water, and 75% ethanol water respectively, extract twice, the liquid-solid ratio is 10 ml / g, carry out three times in parallel at each concentration level, and pass through strong acidic ion exchange resin 732 (1:20=m / m), then eluted with 0.N ammonia water, collected the eluate and evaporated to dryness, then dissolved in water and slowly passed through strong basic anion exchange resin 201×7, eluted with water, evaporated to dryness The active parts of Mori twigs are obtained by dehydration. Among them, the maximum and average amount of active parts of Mori twig obtained by 50% ethanol water extraction is 0.78g.

Embodiment 2

[0038] Example 2 The preparation method of the active part of Morus twig

[0039] Take 5g of mulberry branches, crush, sieve, heat and reflux twice at 60~80°C with 10~20 times of 50%~80% ethanol, each time for 60~180min, filter and concentrate the extract to recover ethanol to obtain the original solution. The stock solution was adsorbed by a strongly acidic cation exchange resin, and then eluted with 0.5N ammonia water, and the eluate was collected and evaporated to dryness to obtain sample 1. Then sample 1 was dissolved in water, slowly passed through a strong basic anion exchange resin, eluted with water, and the eluate was evaporated to obtain sample 2, which is the active part of Mori twig. The results are shown in Table 1.

[0040] Table 1 The amount of fractions with hypoglycemic activity obtained under different extraction conditions

[0041] coding temperature °C time min Alcohol concentration% Liquid-solid ratioml / g Total alkaloids (g) 1...

Embodiment 3

[0043] Example 3 The content determination method of main component-DNJ of mulberry branch active part

[0044] Take 50 μl of the above sample solution, reference solution, and blank solution respectively in a 1.5ml centrifuge tube, add 200 μl of 0.4mol / L potassium borate buffer solution, then add 250 μl of 5 mmol / L FMOC-Cl (50% acetonitrile), shake well , stand at room temperature for 30 minutes, then add 25 μl of 1mol / L glycine (Gly) to stop the reaction, then add 80 μl of acetic acid solution with a volume fraction of 1%, add water to make up to 1000 μl, and finally filter the solution with an organic membrane to obtain the test solution. The test solution is tested by liquid chromatography, and the conditions of liquid chromatography are: chromatographic column: Ultimate TM XB-C18 chromatographic column (4.6 mm × 250 mm); mobile phase: acetonitrile-0.1% acetic acid (38:62); flow rate: 1ml / min; column temperature: room temperature; wavelength: 254nm; injection volume: ...

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Abstract

The invention relates to the field of traditional Chinese medicine active site preparation, and discloses an active site of a mulberry twig, as well as a preparation method and application thereof. The method comprises the following steps: S1, after grinding the mulberry twig, heating, reflowing and extracting for 1-3 times at the temperature of 25-100 DEG C with 10-40 times of 25-95% ethyl alcohol, wherein the every-time extraction time is 60-300 min; filtering and concentrating an extracting solution to obtain a stock solution; S2, enriching and purifying the stock solution by strong-acid cation exchange resin and anion exchange resin in sequence to obtain the active site of the mulberry twig. The preparation method is simple and convenient to operate and good in repeatability, and the content of 1-Deoxynojirimycin (1-DNJ ) can be higher than 30.0%.

Description

technical field [0001] The invention relates to the field of preparation of active parts of traditional Chinese medicines, more specifically, to an active part of Mori twig and [0002] Its preparation method and application. Background technique [0003] Diabetes mellitus is a chronic systemic metabolic disease caused by long-term effects of genetic factors and environmental factors, often resulting in cardiovascular, neurological, eye and other complications. Professor Garry, winner of the Danting Medal, proposed that dyslipidemia is the primary pathological change of diabetes and its complications, and the root cause of the disorder of glucose metabolism in type 2 diabetes is abnormal lipid metabolism, and even called type 2 diabetes "glucolipidosis". . [0004] Morus mulberry is the Moraceae plant mulberry ( Morus alba The twigs of L ) have a long history of medicinal use. There are many types of chemical components contained in mulberry branches, mainly polysacchari...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61K31/445A61P3/10A61P3/04A61P3/06A61P31/12A61K135/00
Inventor 周光雄焦呈亮宋婕张亚群温锦涛叶春玲
Owner JINAN UNIVERSITY
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