DNA extracting method for analyzing MSAP (Methylation Sensitive Amplification Polymorphism) of epiphylla
An extraction method and technology of ginkgo, applied in the field of molecular biology, can solve the problems of high cost, low DNA concentration and low DNA purity, and achieve the effect of high cost and solving pollution
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[0030] Example 1:
[0031] This study was carried out in the Laboratory of Agroecology and Environment, School of Forestry, Shandong Agricultural University in 2011. Weigh about 0.15g of the young leaves of Ginkgo biloba for DNA extraction. The DNA extraction method is as follows:
[0032] 1. DNA extraction preparation
[0033] Mortar, hammer, medicine spoon, 2ml and 1.5ml centrifuge tubes, etc., autoclaving, preparation of 2% CTAB, NaAc and other reagents, preparation of liquid nitrogen, preheating the prepared CTAB in a 65°C water bath for more than 10 minutes .
[0034] 2. Weighing and grinding
[0035] Weigh the fresh tender leaves.015g-0.20g, quickly pour it into a pre-cooled mortar, pour into liquid nitrogen and grind it quickly, grind it several times to a powder, and immediately transfer it to a 2mL centrifuge tube containing 700μL of preheated CTAB, add 35μL of β-mercaptoethanol and 140μL of 20% PVP, mixed well.
[0036] 3. Warm bath
[0037] Incubate at 65°C fo...
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