Method for extracting total flavonoids in hawthorns with ultrasonic wave-microwave-assisted alkali liquor

Abstract

The invention relates to a method for extracting total flavonoids in hawthorns with ultrasonic wave-microwave-assisted alkali liquor, belonging to extraction methods of total flavonoids in hawthorns. The method comprises the following treatment steps of: removing seeds of hawthorn fruits, slicing, drying and grinding the hawthorn fruits; sieving the powder with a 50-mesh sieve; adding 10-12% of alkali liquor according to a solid-to-liquid ratio of 1:(20-30); soaking for 1-2 hours, adopting ultrasonic wave-microwave for extraction, controlling the powder of the ultrasonic wave to be 500W (normally open), controlling the power of the microwave to be 600-800W, carrying out intermittent extraction for 60-90 seconds, leaching and centrifuging the extracting solution, taking the supernatant, then processing the supernatant by a pretreated macroporous adsorption resin; eluting with a certain amount of 70-80% ethanol; carrying out reduced-pressure concentration; crystallizing with ethyl acetate; and carrying out spray drying, thus obtaining total flavonoids in hawthorns. The method is simple in process, combines two technologies which have complementary advantages, and is used for extracting high-purity total flavonoids in hawthorns under the mild and controllable conditions.

Description

Technical field [0001] The present invention involves the method of extraction of natural products, which specializes in a method of ultrasonic-microwave-auxiliary extraction of hawthorn flavonoids. Background technique [0002] Hawthorn, which belongs to the dry and mature fruits of the red -hawthorn of the Rosaceae, is sweet and sour and delicious. It can be used to quench thirst, and can also be used as medicine.Mainly organic acids and flavonoids.Modern pharmacological studies have shown that hawthorn flavonoids have the effects of lowering blood pressure, blood lipids, increased coronary flow, strong heart, and anti -tumor.As a country that produces hawthorn, the source of hawthorn in my country is particularly sufficient and the cost is low. Therefore, the renewable resources of hawthorn are used to develop and produce new drugs. [0003] At present, there are many patents and literatures about the extraction and preparation methods of hawthorn flavonoids. There are boiled ...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many patents and documents on the extraction and preparation methods of total flavonoids of hawthorn, such as boiled alcohol precipitation, dilute alcohol percolation method, hot water extraction method, etc. The overall defect of these extraction methods is that there are many impurities in total flavonoids and the extraction rate is low. , solvent consumption and residual

For example, patent 1062294A discloses a method for extracting total flavonoids from hawthorn leaves. In the process of removing impurities, a small part of total flavonoids from hawthorn is lost, which reduces the yield of the product.