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Novel technology for preparing biodiesel by virtue of low-temperature enzyme fermentation method

A biodiesel and low-temperature technology, applied in the field of oil chemistry, can solve the problems of high cost of separation and purification and enzyme immobilization, low conversion rate of biodiesel, and difference in high temperature resistance of lipase, and achieve low cost, short production cycle and excellent reaction conditions mild effect

Inactive Publication Date: 2015-02-25
龚伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the present invention eliminates the inhibition of short-chain alcohols and glycerol to lipase, prolongs the service life of immobilized enzymes, eliminates the technique of glycerin separation and extraction, reduces production costs, saves time, and improves production efficiency, but due to low Carbon alcohols are toxic to enzymes, resulting in low conversion rate of enzymatic biodiesel, poor high temperature resistance of lipase, high temperature in the production process has a greater impact on enzyme activity, and the cost of enzyme separation and purification and enzyme immobilization is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: A new process for preparing biodiesel by low-temperature enzymatic fermentation, which includes the following steps:

[0018] S1. Put the solidified hogwash oil with an oil content of 95% in the drying room, and use a hot air stove to quickly increase the temperature of the drying room to 145°C, so that the hogwash oil becomes a flowing liquid;

[0019] S2. Put the liquid hogwash oil into the No. 1 reactor with an oil pump, add 1.5% No. 1 biological enzyme composite catalyst in the No. 1 reactor, and the weight ratio of the liquid hogwash oil to the No. 1 biological composite catalyst is 48:1 , reacted for 50min to obtain liquid A, wherein No. 1 biological enzyme composite catalyst was a mixture of lipase and iron sulfate, and its weight ratio was 0.8:1, and the lipase was a lipase produced by bacteria;

[0020] S3. Separating liquid A with a high-speed centrifuge, the rotating speed of the centrifuge is 11000rmp, and the centrifugation time is 30min to remo...

Embodiment 2

[0024] Example 2: A new process for preparing biodiesel by low-temperature enzymatic fermentation, which includes the following steps:

[0025] S1. Put the solidified acidified oil with an oil content of 98% in the drying room, and use a hot air stove to quickly increase the temperature of the drying room to 155°C, so that the acidified oil becomes a flowing liquid;

[0026] S2. Pump the liquid acidified oil into the No. 1 reactor, add 2.5% No. 1 biological enzyme composite catalyst to the No. 1 reactor, and the weight ratio of the liquid acidified oil to the No. 1 biological composite catalyst is 55:1 , reacted for 80min to obtain liquid A, wherein No. 1 biological enzyme composite catalyst was a mixture of lipase and iron sulfate, and its weight ratio was 1.2:1, and the lipase was produced by animal pancreas;

[0027] S3. Liquid A is separated with a high-speed centrifuge, the rotating speed of the centrifuge is 13000rmp, the centrifugation time is 40min, and the residue a...

Embodiment 3

[0031] Example 3:A new process for preparing biodiesel by low-temperature enzymatic fermentation, which includes the following steps:

[0032] S1. Put the solidified waste oil with an oil content of 97% in the drying room, and use a hot air stove to quickly increase the temperature of the drying room to 150°C, so that the waste oil becomes a flowing liquid;

[0033] S2. Put the liquid waste oil into the No. 1 reactor with an oil pump, add 2% No. 1 biological enzyme composite catalyst in the No. 1 reactor, and the weight ratio of the liquid waste oil to the No. 1 biological composite catalyst is 50:1 , and reacted for 60min to obtain liquid A, wherein No. 1 biological enzyme composite catalyst is a mixture of lipase and iron sulfate, and its weight ratio is 1:1, and the lipase is a compound produced by bacteria, fungi and animal pancreas. immobilized enzyme;

[0034] S3. Liquid A is separated with a high-speed centrifuge, the rotating speed of the centrifuge is 12000rmp, the...

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PUM

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Abstract

The invention discloses a novel technology for preparing biodiesel by low-temperature enzymatic fermentation, which comprises the following steps: S1. placing the raw material oil in a drying room, raising the temperature to make the raw material oil into a flowing liquid state; S2. injecting the liquid raw material oil into No. 1 reaction In the kettle, add No. 1 bio-enzyme composite catalyst, and react to obtain liquid A; S3. Centrifuge A liquid to obtain B liquid; S4. Put B liquid into No. 2 reaction kettle, add No. 2 bio-enzyme composite catalyst, methanol, React to obtain crude biodiesel; S5. Centrifuge the crude biodiesel to obtain liquid C; S6. Pour liquid C into No. 3 reactor, decolorize, and filter with plate and frame to obtain biodiesel. The process of the present invention can quickly produce biodiesel by using the low-temperature enzyme fermentation method, turning waste into treasure, and the reaction conditions are mild, no pressurization is required, the reaction temperature of the biological enzyme fermentation is not higher than 78°C, the production cycle is short, and the cost is low and the efficiency is high. , non-toxic, easy to operate, and strong practicability.

Description

technical field [0001] The invention belongs to the field of oleochemistry, and in particular relates to a novel process for preparing biodiesel by a low-temperature enzymatic fermentation method. Background technique [0002] Biodiesel is a kind of biomass energy, which is a kind of mono-alkyl ester of long-chain fatty acid obtained by biological pyrolysis and other technologies. Biodiesel is a mixture of complex organic components with extremely high oxygen content. These mixtures are mainly some organic compounds with large molecular weight, including almost all kinds of oxygen-containing organic compounds. Biodiesel basically does not contain sulfur and aromatics, has a high cetane number, is biodegradable, non-toxic, and harmless to the environment. Its high oxygen-containing CN value is very beneficial to the normal combustion of the compression combustion engine and reduces the emission of harmful substances in the exhaust gas, so it is called a low-pollution fuel. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10G3/00C11C3/10C11C3/04C11B3/00C11B3/02C12P7/64C10L1/02
CPCY02E50/10Y02P30/20
Inventor 龚伟简安华青方洪
Owner 龚伟