A method for extracting sulforaphane from broccoli

Abstract

The invention relates to a method for extracting sulforaphane from broccoli. The method comprises the following steps: (1) dehydrating the edible part of fresh broccoli, drying it to constant weight and crushing to obtain broccoli dry powder; (2) freshly harvested broccoli After the mustard seeds are pulverized, vacuum filtration removes impurities to obtain a crude enzyme solution; (3) add the crude enzyme solution to the broccoli dry powder, stir evenly, extract through enzymolysis and ultrasonic vibration to obtain an extract, and sieve the extract Remove the slag to obtain the filtrate; the filtrate is vacuum filtered to obtain the crude sulforaphane extract; (4) vacuum-concentrate the crude sulforaphane extract to obtain the crude sulforaphane aqueous solution; (5) add acetic acid to the crude sulforaphane extract solution Ethyl extraction to get the ethyl acetate phase, the ethyl acetate phase is vacuum concentrated to a paste, then eluted and then vacuum concentrated to a paste to obtain a paste sulforaphane crude extract; ⑹ paste sulforaphane crude extract The product was freeze-dried to constant weight to obtain crude sulforaphane with a purity of 1% to 15%. The invention is simple, efficient and low in cost.

Description

technical field [0001] The invention relates to a method for extracting and purifying active ingredients in natural products, in particular to a method for extracting sulforaphane from broccoli. Background technique [0002] For a long time, people have regarded vegetables as an important source of vitamins and trace elements for the human body. In recent years, scholars at home and abroad have found that some compounds in cruciferous vegetables have anticancer activity, especially vegetables rich in carotene, such as: carrots, broccoli, broccoli and spinach. Experiments have shown that broccoli ( broccoli ) is rich in anti-cancer substance sulforaphane, which can reduce the formation of tumors in the chest of mice, prevent the activity of carcinogens, and prevent the occurrence of leukemia. [0003] Sulforaphane is an isothiocyanate derivative produced by glucoraphanin (which is a glucosinolate, a type of glucosinolate) by myrosinase hydrolysis or acid hydrolysis, with a ...

AI Technical Summary

Problems solved by technology

At present, the main source of sulforaphane is extracted from broccoli seeds, which costs more than 4,000 yuan per kilogram of seeds, and the cost of raw materials is too high, which is not conducive to industrial production

There have been researches on the artificial synthesis of sulforaphane, but it cannot be mass-produced at present. Secondly, the synthesized sulforaphane is a racemate, which has weakened functions and has certain toxic and side effects.