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A method for inducing eggplant anthers to regenerate haploid plants

A haploid and anther technology, applied in the field of plant biology, can solve the problems of low callus induction rate and differentiation rate, high callus differentiation frequency, and good culture effect, and achieve callus induction rate and differentiation rate High, good callus proliferation and plant propagation effect, high culture efficiency

Inactive Publication Date: 2015-08-19
WUHAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the problems of low callus induction rate and differentiation rate and poor culture effect in the existing anther culture technology, and to provide a method for inducing eggplant anther to regenerate haploid plants, and to obtain callus by applying the method. The probability of wounding tissue is high, the frequency of callus differentiation is high, and the culture effect is good

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  • A method for inducing eggplant anthers to regenerate haploid plants
  • A method for inducing eggplant anthers to regenerate haploid plants
  • A method for inducing eggplant anthers to regenerate haploid plants

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Embodiment 1

[0046] A method for inducing eggplant anthers to regenerate haploid plants, the steps of which are: (test material selection, culture medium design and inoculation culture)

[0047] 1. The source and treatment of test materials:

[0048]The test material of the present invention is selected from eggplant flower buds, collected from the greenhouse of the breeding base of Wuhan Vegetable Science Research Institute, Hubei Province, and the variety code is E83002, which is a first-generation Japanese hybrid eggplant variety. From the flowering stage of eggplant, flower buds are taken from healthy plants at 9:00-10:00 am on a sunny day, and the flower buds with normal development, no damage on the surface, and microspores in the single-nucleate marginal stage are selected for anther culture, corresponding to flower buds The external shape is that the corolla is 1mm-2mm lower than the base of the calyx fissure to the corolla is 1mm-2mm higher than the base of the calyx fissure (see ...

Embodiment 2

[0063] Effects of Low Temperature Pretreatment on Induction Rate of Eggplant Anther Callus

[0064] Eggplant has a very long flowering period (from early May to early October). After the collected anthers have been treated at low temperature (4°C-5°C) for different periods of time, they are inoculated in MS+0.5mg / L2, 4-D+1.0mg / L KT+8mg / L VC + 30.0g / L sucrose + 7.5g / L agar on the most suitable medium, and the results of investigation after 45 days of culture under light conditions showed that (see Table 2), the callus induction rate of anthers treated at different times at low temperature was 1 and Induction rate 2 was significantly increased, but there were significant differences between treatments at different times, which was similar to other plant effects, except that the temperature and time of low temperature were different. This study showed that relative treatment 24h induction rate 1 and induction rate 2 both reached the highest. Therefore, 24h low temperature treatm...

Embodiment 3

[0069] Effects of phytohormones on callus differentiation of adventitious buds:

[0070] In plant tissue culture, NAA is a widely used auxin growth regulator, and the most significant physiological function of cytokinin 6-BA and KT is to promote cell division. Mixed use of auxin and cytokinin can regulate callus differentiation.

[0071] In this study, 6 different concentration combinations of 6-BA, KT and NAA were set up. It can be seen from Table 3 that the effect of the combination of cytokinin KT and NAA is significantly better than that of 6-BA, which is similar to the research results of Zou Jian et al. And the combination of 2.0mg / L KT and 0.01mg / L NAA has the best effect.

[0072] Table 3 Effects of hormones on callus differentiation

[0073]

[0074]

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Abstract

The invention discloses a method for inducing eggplant anther to regenerate a haplobiont. The method mainly comprises the following steps: (1) selecting anther in a proper period and conducting low-temperature pretreatment on the anther; (2) sterilizing the surface of an explant and inoculating; (3) conducting high-temperature dark treatment; (4) conducting light radiation culture and inducing callus to be generated; (5) conducting callus culture proliferation; (6) callus differentiating adventitious buds; (7) grafting bud seedlings, breeding plants in an expanding mode and subculturing; (8) enabling small rootless seedlings to grow roots and become complete plants. The method is high in possibility of obtaining callus, high in callus differentiating frequency, and good in culturing effect. When the method is used in eggplant genetic breeding, the culturing efficiency of eggplant anther can be greatly improved, good eggplant genotype individuals can be enriched, an eggplant haploid breeding method is perfected, a breeding cycle is shortened, and technologies and theoretical basis are supplied for study of genetic breeding and anther in vitro culture of eggplants and other solanaceae crops.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a method for inducing eggplant anthers to regenerate haploid plants. Background technique [0002] Eggplant (Solanum melongena Linn), belonging to the Solanaceae (Solanaceae) genus Solanum (2n=2x=24), originated in the tropical regions of Southeast Asia and is one of the important vegetable varieties in Europe, Asia and North America. It not only has high nutritional value, but also can be eaten in various ways. It is a kind of bulk vegetable that can be combined with dry and fresh, and can be supplied every year. It is economical and affordable, and is very popular among producers and consumers. China is the largest producer of eggplant in the world, therefore, genetic breeding of eggplant is of great significance for actual production. [0003] In the eggplant hybrid breeding work, the selection of inbred lines is very important, but there are often problems such as long bre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王春丽谈太明徐长城黄树苹谈杰张敏陈霞
Owner WUHAN VEGETABLE RES INST
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