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Preparation method of DWD (Double Whey Acidic Protein Domain) antibacterial peptide molecules of fenneropenaeus chinensis

A technology of Chinese prawns and antimicrobial peptides, applied in the field of genetic engineering, can solve the problems of disseminated pathogenicity, badness, enhancement, etc., and achieve the effect of great application potential

Inactive Publication Date: 2013-12-18
INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the preservatives and antibacterial agents widely used in the food industry are chemicals and reagents. These chemicals and reagents exist in the same space as the food packaging. Once absorbed by the human body, they will cause very bad consequences; antibiotics widely used in the pharmaceutical industry At present, it has been exposed that the abuse of antibiotics has continuously stimulated the infection of germs and the enhancement of pathogenicity. Therefore, it is a scientific research goal to find green, safe and biological antibacterial substances that have no impact on human health in the food and pharmaceutical industries. hot issue

Method used

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  • Preparation method of DWD (Double Whey Acidic Protein Domain) antibacterial peptide molecules of fenneropenaeus chinensis
  • Preparation method of DWD (Double Whey Acidic Protein Domain) antibacterial peptide molecules of fenneropenaeus chinensis
  • Preparation method of DWD (Double Whey Acidic Protein Domain) antibacterial peptide molecules of fenneropenaeus chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Recombinant expression of DWD antibacterial peptide of Chinese prawn and detection of antibacterial activity in liquid

[0023] figure 1 Among them, 1 is the total protein of Escherichia coli before induction, 2 is the total protein of Escherichia coli after induction, 3 is the purified recombinant Penaeus chinensis DWD antimicrobial peptide protein, and 4 is the standard protein molecular weight.

[0024] figure 2 Among them, 1 is the E. coli control group, 2 is the E. coli antibacterial test group, 3 is the Pseudomonas aeruginosa control group, 4 is the Pseudomonas aeruginosa antibacterial test group, 5 is the Staphylococcus aureus control group, and 6 is the Staphylococcus aureus control group. Coccus antibacterial test group, 7 is the Candida albicans control group, 8 is the Candida albicans antibacterial test group, * indicates that there is a significant difference between the data.

[0025] 1) Cloning of the gene expression fragment of DWD antimicr...

Embodiment 2

[0032] Example 2: Recombinant expression of Chinese prawn DWD antimicrobial peptide and detection of protease inhibitor activity

[0033] image 3 Among them, 1 is the control without adding any substance on the filter paper sheet, 2 is the control with 15 μL 1×PBS added, 3 is the control with 15 μL BSA protein dialyzate, and 4 is the control with 15 μL recombinant DWD protein dialyzate.

[0034] Figure 4 Among them, 1 is the control without adding any substance on the filter paper sheet, 2 is the control with 15 μL 1×PBS added, 3 is the control with 15 μL BSA protein dialyzate, and 4 is the control with 15 μL recombinant DWD protein dialyzate.

[0035] 1) Cloning of the gene expression fragment of DWD antimicrobial peptide of Penaeus chinensis: Take 3-5 Chinese prawns frozen and preserved, dissect and collect the liver tissue under sterile conditions for 100mg-150mg, and use the animal genome DNA small extraction kit (TaKaRa company Product) for the extraction of Chinese s...

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Abstract

The invention discloses a preparation method of DWD (Double Whey Acidic Protein Domain) antibacterial peptide molecules of fenneropenaeus chinensis, and discloses an application of DWD antibacterial peptide in the aspect of preparing antibacterial proteins and protease inhibitor proteins. The recombined DWD antibacterial peptide of the fenneropenaeus chinensis, which is prepared by utilizing the method disclosed by the invention, can be used for directly inhibiting the growth of common bacteria and inhibiting the activity of proteases secreted in the growth process of the bacteria and has a greater application prospect on the aspects of food antibacterial biological reagents and medical antibiotic substitutes.

Description

technical field [0001] The invention relates to a preparation method of an antimicrobial peptide molecule with double whey acidic protein domain (DWD) in Chinese prawns, and the application of the antibacterial activity and protease inhibitor activity of the DWD antimicrobial peptide molecule, belonging to the technical field of genetic engineering . Background technique [0002] For a long time, there has been an insoluble problem in my country's food and pharmaceutical industries, that is, the serious consequences of preservatives, antibacterial agents, and antibiotics widely used in the food industry and pharmaceutical industry. Most of the preservatives and antibacterial agents widely used in the food industry are chemicals and reagents. These chemicals and reagents exist in the same space as the food packaging. Once absorbed by the human body, they will cause very bad consequences; antibiotics widely used in the pharmaceutical industry At present, it has been exposed t...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/70C12Q1/18C12Q1/02C07K14/435C07K1/22
Inventor 杜志强王金星赵小凡张雪峰王建英
Owner INNER MONGOLIA UNIV OF SCI & TECH
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