A kind of black bone vine extract and its preparation method and application

A kind of extract, the technology of the black bone rattan, applied in the field of black bone rattan extract and its preparation, can solve the problems of large dosage of medicine, large amount of excipients, unfavorable industrial production cost, etc., and achieve the effect of no obvious rebound and good anti-inflammation Effect

Inactive Publication Date: 2016-01-27
SICHUAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The modern research on S. chinensis has also found that its medicinal activity is good, but the active ingredient content in its extract is low. If it is prepared into a prescription, the amount of excipients will be large, which is not conducive to the saving of industrial production costs, and Its dosage is relatively large, which is not convenient for patients to use

Method used

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  • A kind of black bone vine extract and its preparation method and application
  • A kind of black bone vine extract and its preparation method and application
  • A kind of black bone vine extract and its preparation method and application

Examples

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Embodiment 1

[0028] The preparation of embodiment 1 Caulis japonica extract of the present invention

[0029] After pulverizing the vine, leaching with 95% v / v ethanol, filtering, and repeating 3 times, the filtrate was dried under reduced pressure to form an extract, and the extracts were combined. Evenly disperse the 95% ethanol extract into about 2L of distilled water, shake well, and extract with equal volumes of petroleum ether and ethyl acetate respectively. The ethyl acetate extract was dried under reduced pressure to obtain the ethyl acetate extract.

[0030] Take the ethyl acetate extract and mix the sample with 150g of 160-200 mesh silica gel. Put the sample on the silica gel column, and use the chloroform-methanol system for elution. The elution ratios are pure chloroform, chloroform:methanol=10:1, and chloroform:methanol=5:1. Wash 4 column volumes for each gradient, take chloroform:methanol=5:1 eluted part, remove the solvent, and dry to obtain the extract of Caulis sinensis....

Embodiment 2

[0042] Example 2 Screening of the effective parts of Tenghe radix

[0043] 2.3 Extraction, separation and purification of anti-rheumatoid active ingredients

[0044] 2.3.1 Crude extraction of anti-rheumatoid active ingredients

[0045] Method 1: Grind the vine with a pulverizer, use 50% ethanol for reflux extraction, perform reflux extraction according to the solid-liquid ratio of 10:1, reflux at 80°C for one hour, filter after reflux extraction, and repeat reflux extraction Once, filtered, and the combined filtrates were dried under reduced pressure at 60°C for later use.

[0046] Method 2: After crushing Tenghe japonica, extract with 95% ethanol, repeat 3 times, dry the filtrate at 50°C under reduced pressure to form an extract, and combine the extracts. Take about 3g of the extract, spread it on a petri dish, dry it at 105°C to constant weight, do it in parallel 3 times, and calculate the water content.

[0047] Evenly disperse the 95% ethanol extract into about 2L of di...

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Abstract

The present invention provides a periploca forrestii schltr extract preparation method, which comprises: (1) taking a periploca forrestii schltr herb, crushing, extracting with ethanol with a volume ratio of more than 90%, and drying the extract to obtain an ethanol concrete; (2) taking the ethanol concrete, adding water to disperse, extracting by sequentially adopting petroleum ether and ethyl acetate, mixing the ethyl acetate layer, and removing the solvent to obtain an ethyl acetate concrete; and (3) taking the ethyl acetate concrete, adding to a silica gel column, sequentially carrying out gradient elution by adopting chloroform and methanol according to a ratio of (10-5):(0-1), collecting the elution segment at a ratio of chloroform to methanol of 5:1, removing the solvent, and drying to obtain the periploca forrestii schltr extract. The present invention further provides the extract prepared through the method, and a use thereof. The periploca forrestii schltr extract has characteristics of good anti-inflammatory activity, good anti-rheumatoid arthritis activity, significantly increased efficacy activity superior to other extraction sites, and no significant rebound after drug withdrawal, and provides a new selection for clinical medication.

Description

technical field [0001] The present invention relates to a kind of black bone vine extract and its preparation method and use Background technique [0002] Rheumatoid arthritis (RheumatioidArthritis, RA) is an autoimmune deficiency disease characterized by chronic inflammation of the joint synovium. In traditional Chinese medicine, it belongs to the category of "arthritis syndrome". Infection, immune disorders and other factors are related. The incidence of RA in my country is about 0.4%, mostly concentrated in southern provinces. Therapeutic methods include chemical medicine, traditional Chinese medicine, biological agents and other drug treatments and physical therapy. [0003] Black bone vine is the dry root or whole plant of Periploca forrestii Schltr, a plant of the genus PeriplocaLinn. in the family Asclepiasaceae. Commonly used medicine for treating rheumatism and rheumatoid, closed soft tissue injury and other diseases. [0004] The modern research on S. chinensis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/27A61P29/00A61P19/02
Inventor 张宏胡晓荣李琪刘刚张晓喻
Owner SICHUAN NORMAL UNIV
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