A kind of black bone vine extract and its preparation method and application
A kind of extract, the technology of the black bone rattan, applied in the field of black bone rattan extract and its preparation, can solve the problems of large dosage of medicine, large amount of excipients, unfavorable industrial production cost, etc., and achieve the effect of no obvious rebound and good anti-inflammation Effect
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Embodiment 1
[0028] The preparation of embodiment 1 Caulis japonica extract of the present invention
[0029] After pulverizing the vine, leaching with 95% v / v ethanol, filtering, and repeating 3 times, the filtrate was dried under reduced pressure to form an extract, and the extracts were combined. Evenly disperse the 95% ethanol extract into about 2L of distilled water, shake well, and extract with equal volumes of petroleum ether and ethyl acetate respectively. The ethyl acetate extract was dried under reduced pressure to obtain the ethyl acetate extract.
[0030] Take the ethyl acetate extract and mix the sample with 150g of 160-200 mesh silica gel. Put the sample on the silica gel column, and use the chloroform-methanol system for elution. The elution ratios are pure chloroform, chloroform:methanol=10:1, and chloroform:methanol=5:1. Wash 4 column volumes for each gradient, take chloroform:methanol=5:1 eluted part, remove the solvent, and dry to obtain the extract of Caulis sinensis....
Embodiment 2
[0042] Example 2 Screening of the effective parts of Tenghe radix
[0043] 2.3 Extraction, separation and purification of anti-rheumatoid active ingredients
[0044] 2.3.1 Crude extraction of anti-rheumatoid active ingredients
[0045] Method 1: Grind the vine with a pulverizer, use 50% ethanol for reflux extraction, perform reflux extraction according to the solid-liquid ratio of 10:1, reflux at 80°C for one hour, filter after reflux extraction, and repeat reflux extraction Once, filtered, and the combined filtrates were dried under reduced pressure at 60°C for later use.
[0046] Method 2: After crushing Tenghe japonica, extract with 95% ethanol, repeat 3 times, dry the filtrate at 50°C under reduced pressure to form an extract, and combine the extracts. Take about 3g of the extract, spread it on a petri dish, dry it at 105°C to constant weight, do it in parallel 3 times, and calculate the water content.
[0047] Evenly disperse the 95% ethanol extract into about 2L of di...
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