Method for extracting keratins from rabbit hair

An extraction method and keratin technology, applied in the field of extraction of rabbit hair keratin, can solve the problems of low protein quality, practicability discount, denaturation and precipitation, etc., and achieve the effect of simple process, reducing impurities and reducing concentration

Inactive Publication Date: 2014-01-08
KUNSHAN WANFENG GARMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are a series of problems in these methods, such as: salting-out phenomenon, protein denaturation and precipitation leading to protein impurity, etc., resulting in low quality of extracted protein and greatly reduced practicability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting rabbit hair keratin, comprising the steps of:

[0023] Step 1: Hydrogen peroxide pretreatment

[0024] The rabbit hair was soaked in hydrogen peroxide pretreatment solution, the ratio of rabbit hair to hydrogen peroxide pretreatment solution was 1:25, soaked at 65°C for 70min, then rinsed with distilled water and dried.

[0025] Described hydrogen peroxide pretreatment liquid is made of 30% H 2 o 2 , sodium pyrophosphate solution with a concentration of 17g / L, and sodium oxalate solution with a concentration of 3.59g / L are mixed according to the volume ratio of 30%:25%:45%.

[0026] Step 2: Enzyme Treatment

[0027] Adjust the pH of the protease solution (4% of the fiber mass fraction) with ammonia water, soak the rabbit hair pretreated in step 1 in the protease solution, treat at 50°C for 15 minutes, and then rinse with distilled water for at least Twice, and finally oven-dried at 90° C. for 1.5 hours to obtain dry rabbit hair.

[0028] Ste...

Embodiment 2

[0035] Step 1: Hydrogen peroxide pretreatment

[0036] The rabbit hair was soaked in the hydrogen peroxide pretreatment solution, the bath ratio of rabbit hair and hydrogen peroxide pretreatment solution was 1:25, soaked at 75°C for 60min, then the rabbit hair was rinsed with distilled water and dried.

[0037] Described hydrogen peroxide pretreatment liquid is made of 30% H 2 o 2 , sodium pyrophosphate solution with a concentration of 17g / L, and sodium oxalate solution with a concentration of 3.59g / L are mixed according to the volume ratio of 30%:25%:45%.

[0038] Step 2: Enzyme Treatment

[0039] Adjust the pH of the protease solution (4% of the fiber mass fraction) with ammonia water, soak the rabbit hair pretreated in step 1 in the protease solution, treat at 60°C for 20 minutes, and then rinse with distilled water for at least Twice, and finally oven-dried at 90° C. for 1 hour to obtain dry rabbit hair.

[0040] Step 3: ZnCl 2 deal with

[0041] Put the dry rabbit h...

Embodiment 3

[0047] Step 1: Hydrogen peroxide pretreatment

[0048] The rabbit hair was soaked in hydrogen peroxide pretreatment solution, the ratio of rabbit hair to hydrogen peroxide pretreatment solution was 1:25, soaked at 85°C for 50min, then rinsed with distilled water and dried.

[0049] Described hydrogen peroxide pretreatment liquid is made of 30% H 2 o 2 , sodium pyrophosphate solution with a concentration of 17g / L, and sodium oxalate solution with a concentration of 3.59g / L are mixed according to the volume ratio of 30%:25%:45%.

[0050] Step 2: Enzyme Treatment

[0051] Adjust the pH of the protease solution (4% of the fiber mass fraction) with ammonia water, soak the rabbit hair pretreated in step 1 in the protease solution, treat it at 40°C for 10 minutes, and then rinse it with distilled water for at least 10 minutes. Twice, and finally oven-dried at 90° C. for 1.25 hours to obtain dry rabbit hair.

[0052] Step 3: ZnCl 2 deal with

[0053] Put the dry rabbit hair in s...

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PUM

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Abstract

The invention relates to a method for extracting keratins from rabbit hair. The method comprises the following steps: 1, performing hydrogen peroxide pretreatment and drying to obtain dry rabbit hair; 2, performing enzymatic treatment and drying to obtain dry rabbit hair; 3, performing ZnCl2 treatment to obtain a solution of the rabbit hair; 4, extracting the keratins from the rabbit hair to obtain precipitates by adopting an isoelectric precipitation method; 5, flushing the precipitates for multiple times by using distilled water, and performing drying and grinding to obtain keratin powder. The method has the beneficial effects that a three-step treatment method comprising hydrogen peroxide pretreatment, enzymatic treatment and 40 percent ZnCl2 treatment is adopted, and compared with a purer ZnCl2 treatment method, has the advantages that the dissolution time of the rabbit hair is remarkably shortened, and energy consumption in an extraction process is reduced; the precipitates are washed for multiple times by using the distilled water, so that the concentration of Zn<2+> can be remarkably reduced, impurities can be reduced, and the purity of the keratins in the rabbit hair can be improved; the method is simple in process, low in treatment cost and high in rabbit hair keratin extraction rate.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a method for extracting rabbit hair keratin. Background technique [0002] Keratin is a natural protein with good skin-friendly properties, bio-affinity and non-toxicity. It contains a large number of hydrophilic groups such as amino groups and hydroxyl groups. Good application prospects. [0003] Among them, rabbit hair contains a large amount of rabbit hair keratin, which can reach more than 80%. After proper chemical treatment process, the keratin obtained from rabbit hair can be used as the filling material of garment leather, so that the softness and elongation of garment fabric can be significantly improved, while the tensile strength and tear strength of garment fabric can be significantly improved. and the absorbance of the dye did not have a significant effect. Rabbit hair keratin can also be used as a filling material for upper leather to imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/30
CPCC07K14/4741
Inventor 姚建平
Owner KUNSHAN WANFENG GARMENT
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