Contrast medium used for glioma targeted magnetic resonance and fluorescence dual modality imaging, and preparation method thereof

A glioma targeting and dual-mode imaging technology, which is applied in the field of medical testing and biology, can solve the problems of inability to effectively reach the tumor site, insufficient stability, and insufficient specificity of targeting molecules, and achieve superior stability and modifiability Sex, good drug-loading ability, and the effect of achieving targeted imaging

Active Publication Date: 2014-01-15
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(1) The stability of the contrast agent is insufficient, and it is easy to agglomerate
(2) The contrast agent has a large particle size, which is prone to non-specific adsorption and phagocytosis
(3) There is mutual interference between magnetic materials and fluorescent materials, resulting in easy quenching of fluorescence
(4) Insufficient specificity of targeting molecules, unable to effectively reach the tumor site

Method used

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  • Contrast medium used for glioma targeted magnetic resonance and fluorescence dual modality imaging, and preparation method thereof
  • Contrast medium used for glioma targeted magnetic resonance and fluorescence dual modality imaging, and preparation method thereof
  • Contrast medium used for glioma targeted magnetic resonance and fluorescence dual modality imaging, and preparation method thereof

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Embodiment 1

[0069] A glioma-targeted magnetic resonance and fluorescence dual-mode imaging contrast agent and its preparation

[0070] (1) Preparation of superparamagnetic copolymer nanogel: take N-(3-hydroxypropyl)-acrylamide, 10nm superparamagnetic ferric oxide nanoparticles, N, N'-methylenebisacrylamide 1. Dissolve sodium lauryl sulfate in deionized water, stir and blow nitrogen, add potassium persulfate and methacrylic acid, and react at 50-100°C for 4-8 hours. Wherein, the molar ratio of N-(3-hydroxypropyl)-acrylamide to methacrylic acid is 8:1. The reactants were dialyzed with deionized water to remove unreacted monomers, and freeze-dried to obtain freeze-dried powder, which was superparamagnetic copolymer nanogel. Then redissolve with deionized water to obtain a superparamagnetic copolymer nanogel solution with a concentration of 3% (mass / volume).

[0071] (2) Preparation of lactoferrin labeled with fluorescent dye: Dissolve lactoferrin in aqueous sodium bicarbonate solution, add...

Embodiment 2

[0075] A glioma-targeted magnetic resonance and fluorescence dual-mode imaging contrast agent and its preparation

[0076] (1) Preparation of superparamagnetic copolymer nanogel: take N-isopropylacrylamide, 10nm superparamagnetic ferric oxide nanoparticles, N, N'-methylenebisacrylamide, dodecyl Dissolve sodium sulfate in deionized water, stir and feed nitrogen, add potassium persulfate and acrylic acid, and react at 50-100°C for 4-8 hours. Wherein, the molar ratio of N-isopropylacrylamide to acrylic acid is 10:1. The reactants were dialyzed with deionized water to remove unreacted monomers, and freeze-dried to obtain freeze-dried powder, which was superparamagnetic copolymer nanogel. Then redissolve with deionized water to obtain a superparamagnetic copolymer nanogel solution with a concentration of 3% (mass / volume).

[0077] (2) Preparation of lactoferrin labeled with fluorescent dye: Dissolve lactoferrin in aqueous sodium bicarbonate solution, add Cy5.5 solution to it, mix...

Embodiment 3

[0081] A glioma-targeted magnetic resonance and fluorescence dual-mode imaging contrast agent and its preparation

[0082] (1) Preparation of superparamagnetic copolymer nanogel: take N-(2-hydroxypropyl) acrylamide, 20nm superparamagnetic ferric oxide nanoparticles, N, N'-methylenebisacrylamide, Sodium lauryl sulfate is dissolved in deionized water, stirred and blown with nitrogen, potassium persulfate and allylamine are added, and reacted at 50-100°C for 4-8 hours. Wherein, the molar ratio of N-(2-hydroxypropyl)acrylamide to allylamine is 20:1. The reactants were dialyzed with deionized water to remove unreacted monomers, and freeze-dried to obtain freeze-dried powder, which was superparamagnetic copolymer nanogel. Then redissolve with deionized water to obtain a superparamagnetic copolymer nanogel solution with a concentration of 3% (mass / volume).

[0083] (2) Preparation of lactoferrin labeled with fluorescent dye: Dissolve lactoferrin in aqueous sodium bicarbonate soluti...

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Abstract

The invention provides a contrast medium used for glioma targeted magnetic resonance and fluorescence dual modality imaging, and a preparation method thereof. The contrast medium is composed of an acrylamide monomer, an allyl monmer containing sulfhydryl or carboxyl or amidogen, superparamagnetic iron oxide nanoparticles, a fluorochrome and lactoferrin. The preparation method comprises following steps: the acrylamide monomer and the allyl monmer containing sulfhydryl or carboxyl or amidogen are subjected to polymerization reaction so as to obtain a copolymer nanogel; the superparamagnetic iron oxide nanoparticles are added in polymerization reaction process so as to obtain a superparamagnetic copolymer nanogel; the fluorochrome and lactoferrin and combined via covalent bonds so as to obtain fluorochrome labeled lactoferrin; and the superparamagnetic copolymer nanogel and fluorochrome labeled lactoferrin are combined via covalent bonds so as to obtain the contrast medium used for glioma targeted magnetic resonance and fluorescence dual modality imaging. The contrast medium possesses characteristics such as high specificity, selectivity and contrast effect, and low toxic and side effect; and is capable of realizing dual modality imaging.

Description

technical field [0001] The invention relates to the fields of medical examination and biotechnology, relates to a glioma-targeted magnetic resonance and fluorescence imaging contrast agent, in particular to a glioma-targeted magnetic resonance and fluorescence dual-mode imaging contrast agent and a preparation method. Background technique [0002] Glioma is the most common primary malignant tumor in the brain. According to statistics, there are 40,000 to 130,000 new cases of glioma every year in my country. The degree of malignancy is high, and it accounts for the fourth place in the death rate of systemic malignant tumors. At present, surgery is still the preferred treatment for glioma, but clinical practice shows that surgery is difficult to completely remove and has a high morbidity rate, which seriously affects the quality of life and survival rate of patients. Studies have shown that the important reasons for this phenomenon are: ① The preoperative diagnosis of glioma l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/14A61K49/12A61K49/00
Inventor 赵彦兵江凌宇周庆朱艳红杨祥良刘卫
Owner HUAZHONG UNIV OF SCI & TECH
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