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Skin active factor flexible nano-liposome and preparation method and application thereof

A nano-liposome and active factor technology, which is applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., can solve problems such as limiting the scope of cosmetics screening, achieve good skin absorption effect, simple preparation method, high packaging Effect of sealing rate and drug loading

Inactive Publication Date: 2014-01-22
TIANBO MEDICAL TECH SUZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 3. In order to meet the various needs of skin physiological activities, the types of active ingredients in cosmetics usually range from a few to dozens of types. The physical and chemical reactions between active ingredients limit the scope of cosmetic prescription screening

Method used

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  • Skin active factor flexible nano-liposome and preparation method and application thereof
  • Skin active factor flexible nano-liposome and preparation method and application thereof
  • Skin active factor flexible nano-liposome and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Dissolve 120mg of hydrogenated soybean lecithin and 10mg of cholesterol in ether in a water bath ultrasonically, dissolve 0.25mg of vitamin E and BHA mixed antioxidants in a small amount of ethanol, mix with ether, and place on a rotary evaporator to rotate and evaporate to form a film; 1000UI epidermis active factor (EGF) and 1000UI keratinocyte active factor (KGF), 15mg sodium cholate, 1.7mg hyaluronic acid, 500mg lactose were dissolved in pH 7.0 phosphate buffer; add the aforementioned phosphate buffer to the lipid The membrane was vibrated with a vortex oscillator to obtain a preliminary liposome suspension; the liposome suspension was sonicated for 90 cycles with a probe-type ultrasonic cell disruptor (5 seconds for each cycle, 5 seconds for an interval, ice bath ), homogenize 3 times with AVESTIN high-pressure homogenizer, and then pass through 100nm microporous filter membrane 10 times through AVESTIN extruder, and centrifuge the extruded liposome suspension at 10...

Embodiment 2

[0041] Dissolve 100mg of soybean lecithin and 50mg of cholesterol in chloroform by ultrasound in a water bath, dissolve 0.26mg of vitamin E and BHA mixed antioxidants in a small amount of ethanol, and then mix with chloroform; 2000UI of epidermal active factor (EGF), 10mg of sodium cholate , 1.5mg hyaluronic acid, 400mg mannitol are dissolved in the phosphate buffer of pH7.0; The above-mentioned phosphate buffer and chloroform solution are ultrasonically formed into a uniform emulsion in a water bath, and after standing for 30 minutes, rotary evaporation is obtained Plastid suspension; the liposome suspension was sonicated for 60 cycles with a probe-type ultrasonic cell disruptor (5 seconds for each cycle, 5 seconds for an interval, ice bath), and then homogenized with an AVESTIN high-pressure homogenizer for 3 Then pass through the 100nm microporous filter membrane 10 times through the AVESTIN extruder, put the extruded liposome suspension into the dialysis bag (molecular cut-...

Embodiment 3

[0044] Dissolve 10mg of dipalmitoylphosphatidylcholine and 1mg of cholesterol in ether, dissolve 0.25mg of vitamin E and BHA mixed antioxidants in a small amount of ethanol, and then mix with ether; the mixture is evaporated to dryness on a rotary evaporator to form a thin film ;Dissolve 0.1mg of epidermal growth factor (EGF), 0.25mg of sodium cholate, 0.2mg of hyaluronic acid, and 50mg of lactose in phosphate buffer at pH 7.0; add the aforementioned phosphate buffer into the lipid membrane, and place in a vortex The liposome suspension was obtained by vibrating with an oscillator; the liposome suspension was sonicated for 90 cycles with a probe-type ultrasonic cell disruptor (5 seconds for each cycle, 5 seconds for an interval, ice bath), and the AVESTIN high-pressure homogenizer Homogenize 3 times with a homogenizer, then pass through a 100nm microporous filter membrane 10 times through an AVESTIN extruder; centrifuge the extruded liposome suspension at 4°C at 100,000 rpm for...

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Abstract

The invention relates to a skin active factor flexible nano-liposome and a preparation method and application thereof. The flexible nano-liposome comprises the following components in parts by weight: 1-1000 parts of neutral phospholipid, 1-1000 parts of cholesterol, 0.5-500 parts of surfactant, 0.1-20 parts of hyaluronic acid and 0.5-500 parts of skin active factor components. The skin active factor active-component flexible nano-liposome provided by the invention can remarkably improve the stability of the skin active factor components, promote the transdermal absorption of the active components and improve the acting efficiency while increasing the retention and action time of the active components on the surface and deep layer of skin; the skin active factor flexible nano-liposome in a lyophilized powder state is also favorable for application, transportation and storage; the preparation method of the flexible nano-liposome provided by the invention can adopt a mechanical process, and has good stability in the product process and quality and high reproducibility.

Description

technical field [0001] The invention relates to a flexible nano-liposome and its preparation method and application, in particular to a skin active factor-like flexible nano-liposome and its preparation method, as well as the application of the flexible nano-liposome in cosmetics. Background technique [0002] As the understanding of skin physiology and pathology becomes clearer, more and more bioactive animal and plant ingredients and / or biopolymer ingredients are applied to the cosmetics industry, which has achieved huge social and economic benefits. However, to apply these biologically active substances to cosmetics, the following problems need to be solved: [0003] 1. How to solve the stability of these biologically active substances. The stability of such substances is not only determined by their structure and spatial conformation, but also the influence of external environmental factors should not be underestimated. For example, the activity of epidermal active facto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/14A61K8/73A61K8/64A61K8/63A61K8/55A61Q19/00
Inventor 江妍
Owner TIANBO MEDICAL TECH SUZHOU