Method for preparing soy isoflavone aglycones and application of soy isoflavone aglycones to livestock breeding

A technology of isoflavone aglycone and soybean, which is applied in the agricultural field, can solve the problems of few reports on the application of livestock and poultry breeding, high production cost, flavonoid destruction, etc., and achieve the effect of facilitating industrial production, maintaining structural integrity, and improving extraction rate

Active Publication Date: 2014-01-29
广东省天宝生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the acid-base hydrolysis method used is difficult to control, and easily causes flavonoids to be destroyed; Enzyme hydrolysis method has poor stability, low yield, high production cost
[0005] Soybean isoflavones are mainly used in health food, and there are few reports on the application in livestock and poultry breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Take 10kg of commercially available defatted soybean meal, add 50L of ethanol aqueous solution with a volume fraction of 80%, reflux at 70°C, and ultrasonically assist extraction for 3 hours, filter, and recover ethanol from the filtrate to 10L; filter residue with 50L of water, 60°C and Ultrasonic-assisted extraction for 1 hour, filtration, combined filtrates, and centrifugation at 8000 rpm for 10 minutes;

[0039] (2) Pass the centrifugate through 1 kg of macroporous resin D101; extract the precipitate with 3 kg of ethanol aqueous solution with a volume fraction of 80%, filter, and elute D101 with the extract, and collect the eluate;

[0040] (3) Add 150g of hydrochloric acid to the eluent, and reflux at 80°C for 2 hours;

[0041] (4) Recover the ethanol to 0.6L, add 3kg of water, stir evenly, let it settle, centrifuge at 8000 rpm for 10 minutes, and collect the precipitate;

[0042] (5) Dissolve the precipitate with methanol, crystallize and recrystallize after ...

Embodiment 2

[0046] (1) Take 10kg of commercially available defatted soybean germ, add 100L of ethanol aqueous solution with a volume fraction of 90%, reflux at 60°C, and ultrasonically assist extraction for 2 hours, filter, and recover ethanol from the filtrate to 10L; filter residue with 100L of water, 70°C Ultrasonic-assisted extraction for 2 hours, filtration, combined filtrates, and centrifugation at 20,000 rpm for 2 minutes;

[0047] (2) Pass the centrifugate through 2kg of macroporous resin HPD100; extract the precipitate with 6kg of acetone aqueous solution with a volume fraction of 80%, filter, and elute the macroporous resin with the extract, and collect the eluate;

[0048] (3) Add 300g of hydrochloric acid to the eluent, and reflux at 60°C for 5 hours;

[0049] (4) Recover acetone to 0.6L, add 6kg of water and stir evenly, let it settle, centrifuge at 20000 rpm for 2 minutes, and collect the precipitate;

[0050] (5) Dissolve the precipitate with ethanol, crystallize and recry...

Embodiment 3

[0054] (1) Take 10kg of commercially available defatted soybean meal, add 80L of ethanol aqueous solution with a volume fraction of 85%, reflux at 80°C, and ultrasonically assist extraction for 0.5 hours, filter, and recover ethanol from the filtrate to 8L; filter residue with 60L of water, 80°C and Ultrasonic-assisted extraction for 1 hour, filtration, combined filtrates, and centrifugation at 10,000 rpm for 8 minutes;

[0055](2) Pass the centrifugate through 1.5kg of macroporous resin X-5; extract the precipitate with 5kg of methanol aqueous solution with a volume fraction of 90%, filter, and elute the macroporous resin with the extract, and collect the eluate;

[0056] (3) Add 150g of hydrochloric acid to the eluent, and reflux at 80°C for 2 hours;

[0057] (4) Recover methanol to 0.5L, add 5kg of water, stir evenly, let it settle, centrifuge at 10,000 rpm for 8 minutes, and collect the precipitate;

[0058] (5) Dissolve the precipitate with ethanol, crystallize and recry...

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PUM

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Abstract

The invention discloses a method for preparing soy isoflavone aglycones and the application of the soy isoflavone aglycones to livestock breeding. The preparation method comprises the following steps of extracting defatted soy raw materials for many times under the assistance of ultrasonic waves by using an aqueous solution of ethanol and water, and mixing and centrifuging extract; adsorbing centrifugate by using a macroporous resin, extracting centrifuging precipitates, eluting the macroporous resin by using extract liquor, and collecting eluent; adding acids into the eluent, and performing refluxing extraction; concentrating the eluent, adding water, standing a mixed solution, and centrifugally collecting precipitates; dissolving the centrifugally collected precipitates, and performing crystallization and recrystallization; drying crystals to obtain the soy isoflavone aglycones. According to the method, soy isoflavones in two forms are separately extracted, pass through the macroporous resin in a centralized way, and are treated by the acids, so that the interference of components such as phospholipids and proteins is avoided, and the obtained soy isoflavone aglycones are high in extraction rate and purity and easy to industrially produce; when the products are used for livestock breeding, the growth of animals is promoted, the immunity can be improved, the egg yield and the milk yield can be improved, and the quality and the flavor of meat can be improved.

Description

technical field [0001] The invention belongs to the field of agriculture, and in particular relates to a preparation method of soybean isoflavone aglycone and its application in livestock and poultry breeding. Background technique [0002] Soy isoflavones are active ingredients in soybeans that have estrogen-like effects. Anti-oxidation, anti-tumor, prevention of cardiovascular and cerebrovascular diseases, anti-aging and other effects. Its development and utilization have great market value. [0003] There are many extraction methods for soybean isoflavones, and the content of soybean isoflavones obtained by direct alcohol extraction is low, only 1-5%. Through technical improvement, the method of chromatography, macroporous resin adsorption and membrane separation can be used to increase the content of soybean isoflavones to a certain extent. For example, Chinese patent CN1284503 discloses a method for countercurrent extraction of soybean meal and chromatographic purifica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A23K1/16A61P37/04A61P15/08A61P15/14
Inventor 李国清戴述诚叶勇莫泽勇李定清
Owner 广东省天宝生物制药有限公司
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