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A scaffold-type silk fibroin film that is insoluble in water and its preparation and application

一种丝素蛋白、支架型的技术，应用在医药科学、吸收垫、假体等方向，能够解决多孔支架孔壁厚度不均一、丝素蛋白支架力学高、降解速率缓慢等问题，达到利于大量生产、制备工艺简单、减少异物感的效果

Active Publication Date: 2015-09-16
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking sodium chloride particles as the way of denaturation and pore formation as an example, the formed silk fibroin gel is deposited in the gaps of sodium chloride particles. Due to the inhomogeneity of the gaps, the thickness of the pore wall of the porous scaffold is inhomogeneous, from several Micron to hundreds of microns, the formed silk fibroin scaffold has high mechanical properties and a slow degradation rate, but it is not easy to completely wash away a small amount of sodium chloride particles completely wrapped by silk fibroin in the scaffold

Method used

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  • A scaffold-type silk fibroin film that is insoluble in water and its preparation and application
  • A scaffold-type silk fibroin film that is insoluble in water and its preparation and application
  • A scaffold-type silk fibroin film that is insoluble in water and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 (basement membrane 300 μm, scaffold layer 4000 μm)

[0030] (1) Preparation of silk fibroin solution: a) Degumming: Put 100g of silkworm silk (Zhejiang Huazhi Silk Co., Ltd., 5A grade) into 5L of 2M sodium carbonate aqueous solution, bathe in 98°C for 30min, and wash with deionized water , this process was repeated 3 times, the sericin was removed, and the silk fibroin was left, and the silk fibroin was dried at 50°C to obtain 70g of dried silk fibroin for later use; b) dissolving: the above-mentioned dried silk The fibroin is dissolved in 9.3 M lithium bromide (LiBr) aqueous solution at a mass volume ratio of 0.2:1 (that is, 0.2 g of dried silk fibroin is added to each ml of lithium bromide aqueous solution), and the silk fibroin is fully dissolved in a 60°C water bath for 90 minutes to obtain A mixture containing silk fibroin and a small amount of insoluble particles; c) Dialysis: Dialyze the mixture with a regenerated cellulose dialysis bag (molecular weigh...

Embodiment 2

[0035] Example 2 (basement membrane 200 μm, scaffold layer 1000 μm)

[0036] (1) Preparation of silk fibroin solution: a) Degumming: Put 100g of silkworm silk (Zhejiang Huazhi Silk Co., Ltd., 5A grade) into 5L of 2M sodium carbonate aqueous solution, bathe in 98°C for 30min, and wash with deionized water , this process was repeated 3 times, the sericin was removed, and the silk fibroin was left, and the silk fibroin was dried at 50°C to obtain 70g of dried silk fibroin for later use; b) dissolving: the above-mentioned dried silk The fibroin is dissolved in 9.3M lithium bromide (LiBr) aqueous solution at a mass volume ratio of 0.2:1, and the silk fibroin is fully dissolved in a 60°C water bath for 90 minutes to obtain a mixed solution containing silk fibroin and a small amount of insoluble particles; c) Dialysis: The above mixed solution was dialyzed with a regenerated cellulose dialysis bag (molecular weight cut-off 8000 Daltons), dialyzed 12 times with sterile deionized water...

Embodiment 3

[0041] Example 3 (basement membrane 100 μm, scaffold layer 2000 μm)

[0042](1) Preparation of silk fibroin solution: a) Degumming: Put 100g of silkworm silk (Zhejiang Huazhi Silk Co., Ltd., 5A grade) into 5L of 2M sodium carbonate aqueous solution, bathe in 98°C for 30min, and wash with deionized water , this process was repeated 3 times to remove the sericin and leave the silk fibroin, and dry the silk fibroin at 50°C to obtain 70g of dried silk fibroin for later use; b) dissolving: dissolve the silk fibroin by mass Dissolve in 9.3M lithium bromide (LiBr) aqueous solution at a volume ratio of 0.20:1, bathe in water at 60°C for 90 minutes until the silk fibroin is fully dissolved, and obtain a mixed solution containing silk fibroin and a small amount of insoluble particles; c) Dialysis: Dilute the mixed solution with The regenerated cellulose dialysis bag (molecular weight cut-off 2000 Dalton) was dialyzed, and the sterile deionized water of 10 times the volume of the mixed s...

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Abstract

The invention discloses a stent type silk fibroin film insoluble in water, and preparation and application of the stent type silk fibroin film. A preparation method includes the steps of placing silk fibroin solution in a smooth, flat and horizontal mold, and obtaining a basilar film by humid and heat crosslinking; and laying the silk fibroin solution on the surface of the basilar film and forming the stent type silk fibroin film insoluble in water after freezing, drying and humid and heat crosslinking. Silk fibroin is used as a raw material, and biocompatibility is good; a preparation process is non-toxic and environment-friendly, and the thickness of the film and the aperture of a stent layer are easy to control; the film is soft in a moist environment, the basilar film is compact, and cells, microorganisms and biomacromolecules cannot penetrate through the basilar film; the stent layer is in the shape of a porous sponge, and is beneficial to fixation of the film, hemostasis of a wound and regeneration of transmitting tissues; and the film has a wide market in the field of anti-adhesive films and artificial film patches.

Description

(1) Technical field [0001] The invention relates to a silk fibroin film and a preparation method thereof, in particular to a scaffold-type silk fibroin film hardly soluble in water, a preparation method and application thereof. (2) Background technology [0002] Silk is one of the strongest natural fibers, consisting of 70-80% silk fibroin and 20-30% sericin. The amino acid sequence of silk fibroin is mainly composed of repeating units of "glycine-alanine-glycine-alanine-glycine-serine" (GAGAGS), in which glycine, alanine and serine account for more than 85% of the total amino acid content . Most of the repeated sequences are located in the crystallization region of silk fibroin, and self-assemble into an anti-parallel β-sheet structure. These structures endow silk fibroin with the properties of good mechanics and slow degradation rate. [0003] Sericin can cause inflammation in the body and needs to be degummed before use. Silk fibroin is composed of two proteins with a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/56A61L31/04A61L31/16A61L15/32A61L15/42
Inventor 陈隆坤张志红其他发明人请求不公开姓名
Owner ZHEJIANG XINGYUE BIOTECH
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