Resin for separating and purifying tabersonine and applications thereof

Abstract

The invention relates to a resin for separating and purifying tabersonine and applications thereof. A novel resin can be obtained by performing chloromethylation based on a commercial resin as a starting resin, and then reacting with amino acid. The novel resin is extremely low in the capability of adsorbing the tabersonine in the acid liquor extracted from voacanga chalotiana pierre ex stapf seeds, and can selectively adsorb impurities in the extracted acid liquor; when the novel resin is used for separating and purifying tabersonine, the steps are as follows: firstly, pretreating and grinding voacanga chalotiana pierre ex stapf seeds, performing heating reflux extraction and acidification through organic solvent, purifying the acid liquor through the novel resin, enabling the target product tabersonine to be enriched remaining upper column liquor and washing liquor, alkalizing, extracting and concentrating the collecting liquor to obtain the product tabersonine. The novel resin is used for separating and purifying tabersonine, the recovery rate of the tabersonine can be improved, high-concentration alcohol washing is not required in the upper column purification process, and the production cost and the solvent usage amount can be reduced.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of natural products, in particular to a resin for separation and purification and its application. Background technique [0002] Tabersonine, also known as Tabersonine (CAS NO.44289-63-4), is an important alkaloid in African potato fruit, with the molecular formula C 21 h 24 N 2 o 2 , the relative molecular mass is 336.42, and its chemical name is Aspidospermidine-3-carboxylic acid, 2,3,6,7-tetradehydro-(5α, 12β, 19α)-methylseter. Studies have shown that it has antihypertensive effect, and it is currently the main raw material for the synthesis of anticancer drug vinpocetine. [0003] In the patent US3758478, the extraction and separation method of Tabonin is reported, which uses organic solvent extraction, repeated acid water washing, alkalization, organic solvent extraction, recrystallization and other steps to separate and purify Tabonin. The operation process of this meth...

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Problems solved by technology

[0003] In the patent US3758478, the extraction and separation method of Tabonin is reported, which uses organic solvent extraction, repeated acid water washing, alkalization, organic solvent extraction, recrystallization and other steps to separate and purify Tabonin. The operation process of this method is complicated. The yield is low, which is not conducive to industrial production, and toxic solvents such as benzene and chloroform are used, which will cause greater harm to production personnel

[0004] The publication number is CN101250188A, and the name is a patent for the preparation of willow-leaf glycyrrhizin, which introduces the separation and purification of bonin with macroporous resin. This method is feasible in actual production, but the D101 used in the patent method , XDA-6, XDA-7 and other commercial resins lack the adsorption selectivity to its bonin and acid extract impurities, resulting in a large amount of resin, a decrease in the recovery rate of TA bonin, a large amount of solvent, and the extraction solvent used such as n-hexane, Expensive and other issues, increasing production costs