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Extraction method of alpha-zein component

A technology of zein and extraction method, which is applied in the field of extraction of α-zein components, can solve the problems that it is difficult to effectively separate, α-zein is only microgram level and cannot meet industrial applications, and achieves high purity, The effect of high content

Active Publication Date: 2015-01-21
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above purification method can achieve the purpose of purifying α-zein, the amount of α-zein obtained is only microgram level and cannot meet industrial applications.
And the above purification method is difficult to effectively separate riboflavin (Lutein) in zein

Method used

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  • Extraction method of alpha-zein component
  • Extraction method of alpha-zein component
  • Extraction method of alpha-zein component

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1 part of corn flour was dissolved in 5 parts of 50-75% ethanol, and the supernatant was obtained by centrifugation. Add water to dilute until there is no protein precipitation, and then dry to obtain zein. Use solvents such as n-hexane to remove the grease in zein, take 1 part of defatted Zein and dissolve it in 30 parts of N-methylpyrrolidone, and extract repeatedly 3 times with 90 parts of dichloromethane to remove riboflavin. Finally, it was washed with more than 90% ethanol solution, centrifuged to take the supernatant, diluted with water to a concentration below 50%, and filtered; the filtered solid was washed with n-hexane, and vacuum-dried to obtain white α-zein powder. attached figure 1 for riboflavin 1 H NMR figure, this figure illustrates that after extraction 3 times, the residual amount of riboflavin in zein is only 6%. attached figure 2 This is the sodium dodecyl sulfate-acrylamide gel electrophoresis (SDS-PAGE) picture of the obtained white α-zein po...

Embodiment 2

[0023] 1 part of defatted Zein was dissolved in 30 parts of DMSO and washed 5 times in 90 parts of ether to remove riboflavin. Finally, it was washed with more than 90% methanol solution, centrifuged to take the supernatant, diluted with water to a concentration below 50%, and filtered; the filtered solid was washed with petroleum ether, and vacuum-dried to obtain white α-zein powder. attached figure 1 for riboflavin 1 HNMR figure, which shows that after 5 extractions, riboflavin in zein is not detected.

Embodiment 3

[0025] 1 part of defatted Zein was dissolved in 30 parts of NMP, and extracted twice in 90 parts of dichloromethane to remove riboflavin. Finally, it was washed with more than 90% isopropanol solution, centrifuged to take the supernatant, diluted with water to a concentration of less than 50%, and filtered; the filtered solid was washed with n-hexane, and vacuum-dried to obtain white α-zein powder.

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Abstract

The invention relates to a protein extraction technology, and particularly relates to an extraction method of an alpha-zein component. The extraction method is used for solving the technical problem that as the content of the alpha-zein component obtained by using a conventional alpha-zein component purification method is low, the alpha-zein component cannot meet the industrial application and contains more riboflavin. The extraction method mainly comprises the following steps: (1) dissolving zein in N-methylpyrrolidone or DMSO (dimethylsulfoxide); (2) repeatedly extracting the solution in dichloromethane or diethyl ether; (3) dissolving precipitates generated after extraction in above-90% alcohol solution, centrifuging, taking liquid supernatant, and diluting with water to below-50% concentration, and filtering; and (4) washing filtered solids with normal hexane or petroleum ether, and carrying out vacuum drying so as to obtain white alpha-zein powder. The extraction method provided by the invention can be used for effectively separating the riboflavin in the zein, so that the high-purity alpha-zein component is obtained, and the content of the alpha-zein component is relatively high. The extraction method is suitable for industrial production.

Description

technical field [0001] The invention relates to a protein extraction process, in particular to an extraction method of α-zein components. Background technique [0002] Maize is one of the most widespread and important agricultural crops in the world. Corn contains about 10% protein in dry matter, of which 50% to 60% are prolamins. Due to its environmental friendliness, good biocompatibility, low water absorption, high heat resistance and good mechanical properties, zein is widely used as a new material in the fields of medicine, packaging, textile fibers and so on. [0003] Zein exists in the form of a mixture, which consists of four components: alpha, beta, gamma, and delta. In recent years, it has been found that the purified α-fraction has special properties and is especially suitable as a starting material for food, packaging and biopharmaceutical products. The current purification method of α-component is: first, use 70% acetone as solvent, extract at 40°C for 4 hour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/425C07K1/14
CPCC07K14/425
Inventor 宋镕光李帆石彤非
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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