Method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of aqueous enzymatic method

Abstract

The invention discloses a method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of an aqueous enzymatic method, belonging to the technique of medical health-care food production. The method comprises the following steps: (1) extruding and puffing crushed soybean to obtain a puffed product, mixing the puffed product with water and subsequently performing enzymolysis, and performing centrifugal separation to obtain free oil, emulsion, hydrolysate and residues; (2) adding acid into the hydrolysate to perform acid precipitation, subsequently performing centrifugal separation to obtain precipitate and water phase, performing microwave treatment on the water phase, and subsequently performing nanofiltration so as to obtain penetrate; (3) absorbing the penetrate by using macroreticular resin so as to obtain adsorbate; (4) desorbing the adsorbate, subsequently extracting by using acetone so as to obtain extract and unextracted substances, and respectively performing vacuum concentration and spraying drying on the extract and the unextracted substances so as to obtain soy isoflavone and soy saponine. The method is simple in needed equipment and safe to operate, two products of soy isoflavone and soy saponine can be synchronously obtained, and great economic benefits, environment benefits and social benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food manufacturing, and mainly relates to a method for synchronously extracting soybean isoflavones and soybean saponins from aqueous enzymatic hydrolyzate. Background technique [0002] Aqueous enzymatic method is a new oil extraction technology, which destroys the cell wall of plants by means of mechanical and enzymatic hydrolysis, so that the oil can be easily released from the oil plant, because the non-oil components (mainly protein and carbohydrate) in the oil seed have a strong effect on the oil. There is a difference in affinity with water, and at the same time, the oil and non-oil components are separated by using the different specific gravity of oil and water. As a new "safe, green and environment-friendly" oil extraction technology, it has been paid more and more attention by many scholars at home and abroad. It can efficiently recover other valuable components (protein an...

AI Technical Summary

Problems solved by technology

[0006] So far, the enzymatic hydrolyzate has not been comprehensively utilized, and there is no process for synchronously extracting soybean saponin and soybean isoflavones from the enzymatic hydrolyzate, and it is the first time to combine extrusion expansion-microwave heating technology-membrane technology- Combination of resin adsorption technology, simultaneous extraction of active ingredients in soybeans

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