Method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of aqueous enzymatic method

A technology of soybean isoflavones and soybean saponins, which is applied in chemical instruments and methods, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problem that the enzymatic hydrolyzate has not been comprehensively utilized, and achieve significant economic benefits. The effect of improving product yield and operating safety

Active Publication Date: 2014-02-19
山东万得福生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, the enzymatic hydrolyzate has not been comprehensively utilized, and there is no process for synchronously extracting soybean saponin and soybean isoflavones from the enzymatic hydrolyzate, a

Method used

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  • Method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of aqueous enzymatic method
  • Method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of aqueous enzymatic method
  • Method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of aqueous enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] Embodiment: the optimization experiment of microwave heat treatment process

[0035] 1 Materials and methods

[0036] 1.1 Materials and reagents

[0037]

[0038] 1.2 Main instruments and equipment

[0039]

[0040] 1.3 Experimental method

[0041] 1.3.1 Determination of soybean components

[0042] Determination of moisture: GB304-87; Determination of crude fat: Soxhlet extraction method in GB5512-85; Determination of crude protein: GB6432-94 standard method; Determination of ash: GB5009.4-85.

[0043] 1.3.2 Process flow (see figure 1 )

[0044] 1.3.3 Calculation formula

[0045]

[0046] In the formula, the supernatant is the supernatant after extracting soybean peptide and soybean oil, heating and centrifuging by microwave.

[0047] 2 Results and Discussion

[0048] 2.1 Effect of microwave heating treatment on the extraction rate of soybean isoflavones and soybean saponins

[0049] 2.1.1 The effect of microwave heating time on the extraction rate of ...

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Abstract

The invention discloses a method for synchronously extracting soy isoflavone and soy saponine from hydrolysate of an aqueous enzymatic method, belonging to the technique of medical health-care food production. The method comprises the following steps: (1) extruding and puffing crushed soybean to obtain a puffed product, mixing the puffed product with water and subsequently performing enzymolysis, and performing centrifugal separation to obtain free oil, emulsion, hydrolysate and residues; (2) adding acid into the hydrolysate to perform acid precipitation, subsequently performing centrifugal separation to obtain precipitate and water phase, performing microwave treatment on the water phase, and subsequently performing nanofiltration so as to obtain penetrate; (3) absorbing the penetrate by using macroreticular resin so as to obtain adsorbate; (4) desorbing the adsorbate, subsequently extracting by using acetone so as to obtain extract and unextracted substances, and respectively performing vacuum concentration and spraying drying on the extract and the unextracted substances so as to obtain soy isoflavone and soy saponine. The method is simple in needed equipment and safe to operate, two products of soy isoflavone and soy saponine can be synchronously obtained, and great economic benefits, environment benefits and social benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food manufacturing, and mainly relates to a method for synchronously extracting soybean isoflavones and soybean saponins from aqueous enzymatic hydrolyzate. Background technique [0002] Aqueous enzymatic method is a new oil extraction technology, which destroys the cell wall of plants by means of mechanical and enzymatic hydrolysis, so that the oil can be easily released from the oil plant, because the non-oil components (mainly protein and carbohydrate) in the oil seed have a strong effect on the oil. There is a difference in affinity with water, and at the same time, the oil and non-oil components are separated by using the different specific gravity of oil and water. As a new "safe, green and environment-friendly" oil extraction technology, it has been paid more and more attention by many scholars at home and abroad. It can efficiently recover other valuable components (protein an...

Claims

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Application Information

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IPC IPC(8): A61K36/48C07J63/00C07H15/256C07H1/08A23L1/30A23L33/10
Inventor 江连洲李杨隋晓楠王中江齐宝坤冯红霞张雅娜曹亮赵城彬
Owner 山东万得福生物科技有限公司
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