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Solvent extraction method for active polysaccharide of wheat bran

A technology of wheat bran and active polysaccharides, applied in organic active ingredients, pharmaceutical formulas, allergic diseases, etc., can solve problems such as environmental pollution, lack of economic feasibility of recycling, etc., achieve rich sources, significant immune regulation function, and industrialization The effect of developing a bright future

Active Publication Date: 2014-02-19
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in future industrial applications, high NaOH concentration and high temperature extraction will bring ecological and environmental protection problems
The sodium salt generated after NaOH neutralization, if discharged directly, will cause serious pollution to the environment, and the recovery is not economically feasible.

Method used

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  • Solvent extraction method for active polysaccharide of wheat bran
  • Solvent extraction method for active polysaccharide of wheat bran
  • Solvent extraction method for active polysaccharide of wheat bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the solvent method extraction of wheat bran active polysaccharide

[0035] 1. Polysaccharide extraction: the flow chart of polysaccharide extraction is as follows figure 1 shown.

[0036] 1) Pretreatment of wheat bran

[0037] Weigh 100 g of wheat bran, disperse it in 500 mL of water, stir at room temperature for 30 minutes, pass through a 60-mesh sieve, and collect the sieved material; repeat this process 3-5 times until the water is clear, and the wheat bran with deendosperm starch is obtained.

[0038] 2) Acid hydrolysis

[0039] The starch-removed wheat bran was dispersed into 800 mL of citric acid solution with a concentration of 0.15 M, placed in a 50.0° C. insulation tank and stirred for 3.0 h to obtain a mixed solution of acid-hydrolyzed wheat bran.

[0040] 3) Alkaline extraction

[0041] Add KOH to the acid-hydrolyzed wheat bran mixed solution to adjust the concentration of the alkali solution to 0.08M; place it at 40.0°C and stir for extract...

Embodiment 2

[0075] Embodiment 2, the solvent method extraction of wheat bran active polysaccharide

[0076] 1. Polysaccharide extraction: the flow chart of polysaccharide extraction is as follows figure 1 shown.

[0077] 1) Pretreatment of wheat bran

[0078] Weigh 1000g of wheat bran, disperse it in 6L of water, stir at room temperature for 30min, pass through a 60-mesh sieve, and collect the sieve; repeat this process 4 times until the water is clear, and the wheat bran with degerminated starch is obtained.

[0079] 2) Acid hydrolysis

[0080] Disperse the destarched wheat bran into 10L of citric acid solution with a concentration of 0.05M, place it in an insulated tank at 80.0°C and stir for 1.0h to obtain a mixed solution of acid-hydrolyzed wheat bran.

[0081] 3) Alkaline extraction

[0082] Add KOH to the acid-hydrolyzed wheat bran mixed solution to adjust the concentration of the alkali solution to 0.05M; place it at 50.0°C and stir for extraction, and the extraction time is 0....

Embodiment 3

[0102] Embodiment 3, the solvent method extraction of wheat bran active polysaccharide

[0103] 1. Polysaccharide extraction: the flow chart of polysaccharide extraction is as follows figure 1 shown.

[0104] 1) Pretreatment of wheat bran

[0105] Weigh 10 kg of wheat bran, disperse it in 4.5 L of water, stir at room temperature for 30 minutes, pass through a 60-mesh sieve, and collect the sieve; repeat this process 5 times until the water is clear, and the wheat bran with degerminated starch is obtained.

[0106] 2) Acid hydrolysis

[0107] Disperse the destarched wheat bran into 120 L of citric acid solution with a concentration of 0.10 M, place it in a heat preservation tank at 60.0° C. and stir for 2.4 hours to obtain a mixed solution of acid-hydrolyzed wheat bran.

[0108] 3) Alkaline extraction

[0109] Add KOH to the acid-hydrolyzed wheat bran mixed solution to adjust the concentration of the alkali solution to 0.01M; put it at 60.0°C and stir for extraction, and th...

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Abstract

The invention discloses a method for extracting active polysaccharide-araboxylan from wheat bran through a compound solvent. The extraction method mainly comprises the following steps: the wheat bran is mildly hydrolyzed and preprocessed by a weak acid (citric acid); the target polysaccharide is extracted through a strong alkali (KOH); the extracting solution is centrifugated, neutralized, concentrated and subjected to ethanol precipitation; the ethanol precipitate is dried to obtain the target polysaccharide. The centrifuged supernate of the ethanol precipitate is concentrated and dried, and then can be used as an agricultural compound fertilizer for comprehensive utilization. According to the invention, a large quantity of the byproduct resource, namely the wheat bran is utilized, and the compound solvent is designed to extract the biological active polysaccharide, namely the araboxylan. Compared with the traditional alkali method, the extraction method improves the working efficiency, and reduces the environmental protection pressure. Animal experiments prove that the polysaccharide has prominent immunoregulatory activity, and the effect is comparable to that of lentinan. According to the target polysaccharide provided in the invention, the raw material is low in cost and rich, and the extraction method is environment-friendly, so that the industrialization development of the polysaccharide is promising in the future.

Description

technical field [0001] The invention relates to a green and environment-friendly solvent extraction method of arabinoxylan extracted from wheat bran and having immunoregulatory activity. Background technique [0002] Wheat bran is a large by-product of wheat flour milling. my country, as a major producer and consumer of wheat, has about 20 million tons of bran resources every year. Wheat bran is mainly composed of tissue parts such as seed coat, aleurone layer and residual endosperm of wheat grain, and nearly half of its composition belongs to fibrous matrix, including cellulose, lignin and the main non-starch and non-starch in the cell wall structure of cereal crops. Cellulose polysaccharide-arabinoxylan (or pentosan), wherein arabinoxylan accounts for more than 60% of the wheat bran fiber matrix. A large number of previous studies at home and abroad have shown that arabinoxylan in wheat bran is an important functional polysaccharide. In addition to having an important imp...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02
Inventor 周素梅王凤忠钟葵刘丽娅佟立涛周闲容
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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