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A method for extracting and preparing peat fulvic humic acid and its medicinal application

An extraction method, the technology of fulvic acid, which is applied in the direction of drug combination, antipyretics, antineoplastics, etc., can solve the problems of easy pollution of resin, difficulty in industrial production, large amount of waste water, etc., and achieve the effect of avoiding high temperature

Inactive Publication Date: 2015-10-28
云南联合药业有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the less extraction amount in the separation process of fulvic acid in the prior art, the purity of the separated fulvic acid is not high, the alcohol solvent with a low boiling point has a large volatilization loss, and the ion exchange resin The resin in the refining process is easy to pollute, the amount of waste water is large, it is not easy to industrialize production, and the method of microbial degradation has many defects such as long fermentation time and low product purity. Provide a method for extracting and preparing peat fulvic acid and its medicinal use

Method used

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  • A method for extracting and preparing peat fulvic humic acid and its medicinal application
  • A method for extracting and preparing peat fulvic humic acid and its medicinal application
  • A method for extracting and preparing peat fulvic humic acid and its medicinal application

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Embodiment 1

[0051] A method for extracting and preparing peat yellow humic acid;

[0052] The peat in this embodiment is Yunnan Shiping peat; the mass ratio of peat and water in the aqueous solution of peat is 1:5; the strains used are shown in the following table:

[0053]

[0054]

[0055]

[0056] Described extraction method comprises the following steps:

[0057] (1) Place each bacterial strain in culture medium and cultivate it to the logarithmic growth phase; the medium includes: 10 g of fulvic acid, 10 g of agar powder, 5 g of peptone, and 1000 mL of deionized water; at 38 ° C and a pressure of 0.1 MPa, Shake and acclimatize at a shaking speed of 300rpm. After culturing to the logarithmic phase, carry out 10-fold volume expansion according to three levels of I, II, and III. The medium for expansion includes: 10g of fulvic acid, 5g of agar powder, and peptone 5g, 1000mL deionized water;

[0058] (2) Flow extreme thermophilic bacteria, barophilic bacteria, halophilic bacte...

Embodiment 2

[0061] A method for extracting and preparing peat yellow humic acid;

[0062] The peat in the present embodiment is Zoige peat in Sichuan; the mass ratio of peat and water in the aqueous solution of peat is 1:10; the strains used are as shown in the table below:

[0063]

[0064]

[0065] Described extraction method comprises the following steps:

[0066] (1) Place each bacterial strain in the culture medium and cultivate it to the logarithmic growth phase; wherein, the culture medium includes: 10 g of fulvic acid, 10 g of agar powder, 5 g of peptone, and 1000 mL of deionized water; Under the condition of 0.1MPa, shake and acclimatize at a shaking speed of 300rpm. After culturing to the logarithmic phase, in the seed tank, perform 10-fold volume expansion according to three levels of I, II, and III. The medium for expansion includes: Fulvic acid 10g, agar powder 10g, peptone 5g, deionized water 1000mL;

[0067] (2) Flow extreme thermophilic bacteria, barophilic bacteri...

Embodiment 3

[0070] A method for extracting and preparing peat yellow humic acid;

[0071] The peat in the present embodiment is Heilongjiang birch forest peat, and the mass ratio of peat and water in the aqueous solution of peat is 1:2; The strains used are shown in the following table:

[0072]

[0073]

[0074] Described extraction method comprises the following steps:

[0075] (1) Place each bacterial strain in the culture medium and cultivate it to the logarithmic growth phase; wherein, the medium includes: 10g of fulvic acid, 1-10g of agar powder, 5g of peptone, 1000mL of deionized water; at 35°C Under the conditions of 38°C and a pressure of 0.1MPa, shake and acclimatize at a shaking speed of 300rpm. After culturing to the logarithmic phase, perform 10-fold volume expansion in the seed tank according to three levels of I, II, and III;

[0076] (2) Flow extreme thermophilic bacteria, barophilic bacteria, halophilic bacteria, alkalophilic bacteria and acidophilic bacteria into ...

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Abstract

The invention discloses an extraction method of peat fulvic acid and drug application. A method A is that mixing peat, hyperthermophilic baceria (3-13 CFU), barophilic baceria (5-11 CFU), halophilic baceria (6-12 CFU), basophilic baceria (7 to 13 CFU) and acidophilic bacteria (3-10 CFU) under three conditions including the condition I (10-130 DEG C and 0.05-0.18 MPa), the condition II (5-75 DEG C and 0.05 to 0.12 MPa) and the condition III (5-65 DEG C and 0.05 to 0.12 MPa) and treating for 1-2, 1-3 and 1-2 days respectively; a method B is that mixing the peat and part of the baceria under the conditions I and II for 1-2 and 1-3 days respectively, and mixing with the acidophilic bacteria under the condition III for 1-2 days; a method C is that mixing the peat, the hyperthermophilic baceria and the barophilic baceria under the condition I for 1-3 days, mixing with the halophilic baceria and the basophilic baceria under the condition II for 1-3 days, and mixing with the acidophilic bacteria under the condition III for 1-2 days. Fulvic acid prepared by the method is high in purity.

Description

technical field [0001] The invention relates to a method for extracting and preparing peat fulvic humic acid, a product obtained therefrom, and a medicinal application. Background technique [0002] Peat humic acid belongs to the biopolymers with multi-component, multi-fraction and semi-colloid characteristics transformed from the remains of swamp plants (from Biopolymers, [Fen] M. Hovritt [De] A. Steinbicher Editor-in-Chief, Translated by Guo Shengrong, Chemical Industry Press, Modern Biotechnology and Medical Science and Technology Publishing Center, 2004.6 First Edition, Volume 1 Lignin, Humus and Coal). Peat humic acids containing carboxyl, phenolic hydroxyl, quinone and other functional groups can be classified into yellow humic acid, brown humic acid and black humic acid. Fulvic acid has a smaller molecular weight and more active functional groups. [0003] In the 1980s and 1990s, Beijing Haidian Hospital, Tongren Hospital, the Affiliated Hospital of Heilongjiang Uni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08H99/00C07G99/00C12N1/20C12N1/14A61K35/10A61P1/04A61P19/02A61P29/00A61P35/00A61P35/02A61P37/04
Inventor 吕伟东杨俊佳王桥山周霞萍刘强
Owner 云南联合药业有限责任公司