Preparation of sugar alcohol organic selenium-rich foliar fertilizer by fermentation method
A technology of selenium-enriched foliar fertilizer and fermentation method, applied in the field of agriculture and forestry production, can solve the problems of low bioavailability, low titer, water quality and environmental pollution, etc., and achieve the effects of increasing product yield and promoting crop growth.
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specific Embodiment approach 1
[0016] Step 1: under stirring, add 10 parts of carbon source (mass mixture of tapioca starch, cornstarch, molasses, etc.) and nitrogen source (urea and ammonium chloride are mixed in a mass ratio of 2:1) to a fermenter equipped with 80 parts of distilled water 4 parts, 0.2 parts of inorganic salt (mixed with magnesium sulfate, potassium dihydrogen phosphate, etc.), fully stirred and dissolved, sterilized at 130°C for 25 minutes, and left to cool.
[0017] Step: 2: Insert the activated Torula sporogenes strain, the inoculum amount is 10%, the ventilation rate is 7L / min, cultivate at 28°C for 30 hours, and add 20 parts of sterilized supplements respectively at 8 hours, 12 hours and 18 hours after inoculation. Sterilized after fermentation.
[0018] Step 3: Add 0.5 parts of maltotriitol, 1.5 parts of sweet alcohol, 8 parts of potassium dihydrogen phosphate, 8 parts of potassium nitrate, 0.1 part of surfactant, and 0.1 part of diethyl diethylhexanoate into the fermentation broth, ...
specific Embodiment approach 2
[0019] Step 1: under stirring, add 10 parts of carbon source (mass mixture of tapioca starch, chitosan oligosaccharide, sucrose, etc.) and nitrogen source (urea and ammonium sulfate are mixed in a mass ratio of 2: 1) to a fermenter equipped with 80 parts of distilled water 4 parts, 0.1-1 part of inorganic salt (magnesium sulfate, potassium dihydrogen phosphate, etc. mixed in mass), fully stirred and dissolved, sterilized at 130°C for 25 minutes, and left to cool.
[0020] Step: 2: Insert the activated Torula sporogenes strain, the inoculum amount is 10%, the ventilation rate is 6L / min, cultivate at 25°C for 25-35h, and add sterilized feed 20 h, 12h, and 18h after inoculation, respectively. share. Sterilized after fermentation.
[0021] Step 3: Add 0.5 parts of maltotriitol, 1.5 parts of allicitol, 10 to 20 parts of potassium dihydrogen phosphate, 10 to 20 parts of potassium nitrate, 0.1 parts of surfactant, and 0.1 parts of diethyl diethylhexanoate to the fermentation broth...
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