Factory-like production method of silkworm cordyceps

A production method and industrialized technology, which is applied in the industrialized production process of silkworm Cordyceps, can solve the problems of long processing time, low success rate, difficult growth of strains, etc., and achieve the effect of simple process

Inactive Publication Date: 2014-03-19
钱国琛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The weak point of this method is, 1) put silkworm into the silkworm pretreatment time of 20~50min under ultraviolet sterilization too long; Bombyx mori outflow body fluid; 3) Put the inoculated silkworm into the nutrient soil and place it in the dark at 5°C-12°C for cultivation, which will make the silkworm body flow out more body fluid and only the skin of the silkworm body will remain
The disadvantage of this patent is that after the physical and ch...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The production site is repaired and cleaned, the water supply and drainage system is cleaned and disinfected, the circuit system is inspected and repaired, the ventilation system is cleaned, the culture rack and equipment used are repaired and cleaned, and the disinfection standard is the highest.

[0027] Prepare silkworm larvae: select robust living silkworms 5 days after the fifth molt; in a sterile environment, first wipe the larvae with 75% ethanol solution for disinfection; then rinse the sterilized worms with sterile water. body; then dry the worm body in a sterile environment; store the dried worm body at -2°C for future use.

[0028] Prepare PDA medium: wash 200g of peeled potatoes, add 1000mL of sterile distilled water, add 20g of glucose, add 20g of agar, soak, sterilize, and cool for later use.

[0029] Prepare rice culture medium: Wash 36 grams of rice, add it to a 380ml jam bottle, add 50ml water at the same time, cover it, and sterilize it under normal pr...

Embodiment 2

[0036] The production site is maintained and arranged, which is the same as in Example 1.

[0037] Preparation of silkworm larvae, preparation of PDA nutrient medium, preparation of rice medium, preparation of inoculum and preparation of other materials are all the same as in Example 1. Just don't prepare to sterilize the sandy soil. Specific steps are as follows:

[0038] All venues and materials are prepared for disinfection and inoculation. Draw 0.01ml of the inoculum with the prepared syringe and puncture the prepared gastropods of the silkworm larvae; place the inoculated silkworm larvae on the prepared rice culture medium neatly at an angle of 45°, cover and culture. First cover it with the prepared black paper, put it in an environment with a temperature of -2°C and a humidity of 85%, and cultivate it for 10 days, and then cultivate it for 15 days in an environment of 10°C, until the white mycelium is covered with worms; The temperature is 20°C and the humidity is 65...

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PUM

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Abstract

The invention provides a factory-like production method of silkworm cordyceps. The method is characterized by comprising the steps as follows: separating fruiting bodies of cordyceps militaris, sterilizing and washing the fruiting bodies, putting the fruiting bodies on a slant culture medium, and placing the slant culture medium in an incubator for culture; placing a PDA (potato dextrose agar) culture medium in a shake flask, placing white mycelia in the shake flask for shake culture, inoculating the white mycelia on a rice culture medium, after the white mycelia grow over the rice culture medium, taking out the mycelia, adding a glucose liquid, mashing the mycelia, and obtaining a nutrition fungus suspension as an inoculation fluid; stabbing and injecting pleopod parts of silkworm larvae with a medical injector sucked with the inoculation liquid to obtain the inoculated silkworm larvae, obliquely placing the silkworm larvae on the sterilized rice culture medium, performing light-shielding variable-temperature culture on the silkworm larvae firstly, and performing light culture after white hyphae grow over the larvae; and collecting the silkworm cordyceps. The factory-like production method is simple in technology, the mass production can be realized, and the silkworm cordyceps with shapes similar to natural cordyceps sinensis and medium values basically identical with the cordyceps sinensis can be produced.

Description

technical field [0001] The invention is related to indoor artificial cultivation of Cordyceps sinensis, and is an industrialized production process of Cordyceps sinensis by using silkworm larvae to produce Cordyceps fungus indoors. The product produced by this process is the conjugation of insect body (silkworm larvae) and grass body (fruiting body), which is similar in shape to naturally growing Cordyceps sinensis, and its main components and medicinal value are basically the same. Background technique [0002] The Chinese patent with the publication number CN103262751A discloses a method for cultivating Cordyceps silkworms by infecting silkworm larvae with the Cordyceps militaris strain, which includes the following steps: (1) selection of silkworms: selecting silkworms of the fifth instar 3rd to 5th days as hosts; 2) Strain preparation: inoculate the strain of Cordyceps militaris in the liquid culture medium, and after shaking culture, filter the sterile gauze to obtain t...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 钱国琛
Owner 钱国琛
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