Feed capable of reducing methanogenesis of gastrointestinal tracts of buffalos
A gastrointestinal, methane technology used in the field of ruminant feed formulation
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Embodiment 1
[0009] Apply the feed of the present invention to simulate the rumen digestion and metabolism of buffalo in vitro and apply the bottle system to simulate the rumen digestion and metabolism of buffalo in vitro. The method adopts the pressure reading type in vitro gas production system (Reading pressure system, RPT) of Mauricio et al. (1999) to carry out in vitro rumen fermentation and cultivation, The anaerobic artificial rumen buffer was prepared according to the method of Menke and Steingass et al. (1988). The substrate formulation is shown in Table 1 below.
[0010] The rumen fluid comes from three buffaloes with a body weight of about 400±5kg installed with permanent rumen fistulas as donor animals for rumen fluid. The experimental cows were fed in a tethered manner, fed twice a day at 8:00 and 17:00, had free access to water, conventional light, deworming and management.
[0011] Extract 10 μl of gas after substrate fermentation for 6 hours, 12 hours, and 24 hours. Use ma...
Embodiment 2
[0019] Apply the feed of the present invention to simulate the rumen digestion and metabolism of buffalo in vitro and apply the bottle system to simulate the rumen digestion and metabolism of buffalo in vitro. The method adopts the pressure reading type in vitro gas production system (Reading pressure system, RPT) of Mauricio et al. (1999) to carry out in vitro rumen fermentation and cultivation, The anaerobic artificial rumen buffer was prepared according to the method of Menke and Steingass et al. (1988). The substrate formulation is shown in Table 3 below.
[0020] Table 3. Feeds of the control group and the experimental group (air-dried substance basis, mass percentage) (%)
[0021]
[0022] The rumen fluid comes from three buffaloes with a body weight of about 400±5kg installed with permanent rumen fistulas as donor animals for rumen fluid. The experimental cows were fed in a tethered manner, fed twice a day at 8:00 and 17:00, had free access to water, conventional li...
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