Method for producing beer yeast for feeds by fermenting high-salt-content amino acid wastewater
A technology of brewer's yeast and amino acids, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable treatment of amino acid wastewater, high cost of concentration treatment process, high investment and operation costs, and achieve nitrogen source The effect of high utilization efficiency, high application promotion potential, and strong competitive advantage
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0025] Embodiment 1: Acclimatization and cultivation of brewer's yeast strains for feed.
[0026] 1. Take the waste liquid from an amino acid factory in Wuhan, after the hydrolysis of hair acid to produce cysteine, and use it as the high-salt amino acid wastewater required below. Its composition table is as follows:
[0027] Table 1 Contents of related elements in amino acid wastewater
[0028] project name
unit
Test results
Method basis
Total Nitrogen (N)
%
8.79
GB / T8572-2010
Sodium (Na)
%
0.52
ICP method
Chlorine (Cl)
g / L
131.26
AgCl nephelometric method
Lead (Pb)
mg / kg
0.91
ICP method
Arsenic (As)
mg / kg
0.076
Mercury (Hg)
mg / kg
0.014
Chromium (Cr)
mg / kg
1.51
ICP method
Cadmium (Cd)
mg / kg
0.06
ICP method
[0029] Table 2 Amino acid composition and content ...
Embodiment 2
[0037] Fermentation of brewer's yeast in liquid medium with glucose as carbon source and amino acid wastewater as nitrogen source.
[0038] 1. Prepare a liquid fermentation medium whose concentration of high-salt amino acid wastewater is 200g / L, wherein the high-salt amino acid wastewater is the only nitrogen source (its source and composition are consistent with Example 1), and the liquid fermentation medium is also added Other chemical substrates required by microorganisms, the components are: glucose: 50g / L, KH 2 PO 4 : 9g / L, yeast powder: 6g / L, MgSO 4 : 5g / L, CaCO 3 : 1.2g / L, FeSO 4 : 2.5g / L, the medium pH is 5.5.
[0039] 2. Inoculate the domesticated brewer's yeast strain obtained from Example 1 into the liquid fermentation medium prepared in step 1 according to the inoculum size of 2%, in the fermenter at 28°C, the rotating speed is 200rpm / min, and the aeration coefficient is 0.5 Under the conditions of liquid fermentation culture 5d. After the cultivation, the ce...
Embodiment 3
[0041] Fermentation of brewer's yeast in a liquid medium with cornstarch enzymatic hydrolyzate as carbon source and amino acid wastewater as nitrogen source.
[0042] 1. Prepare a liquid fermentation medium whose concentration of high-salt amino acid wastewater is 200g / L, wherein the high-salt amino acid wastewater is the only nitrogen source (its source and composition are consistent with Example 1), and the liquid fermentation medium is also added Other chemical substrates required by microorganisms, the components are: corn starch enzymatic hydrolysis solution 50g / L (according to the dry weight of corn starch), KH 2 PO 4 : 9g / L, yeast powder: 6g / L, MgSO 4 : 5g / L, CaCO 3 : 1.2g / L, FeSO 4 : 2.5g / L, the medium pH is 5.5.
[0043] 2. Inoculate the acclimated brewer's yeast strain obtained from Example 1 into the liquid fermentation medium prepared in step 1 according to the inoculum size of 5%, in the fermenter at 28°C, the rotating speed is 200rpm / min, and the aeration coe...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com