Method for producing beer yeast for feeds by fermenting high-salt-content amino acid wastewater

A technology of brewer's yeast and amino acids, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable treatment of amino acid wastewater, high cost of concentration treatment process, high investment and operation costs, and achieve nitrogen source The effect of high utilization efficiency, high application promotion potential, and strong competitive advantage

Inactive Publication Date: 2014-03-19
HUBEI UNIV OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported amino acid wastewater treatment methods mainly include electrodialysis, ultrafiltration, biochemical methods, etc., but the shortcomings of electrodialysis and ultrafiltration limit the promotion of this method due to high investment and operating costs and inconvenient maintenance; and biochemical methods Because of its low investment and operating costs, mature technology, no secondary pollution, and convenient operation and management, it has received worldwide attention in organ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Acclimatization and cultivation of brewer's yeast strains for feed.

[0026] 1. Take the waste liquid from an amino acid factory in Wuhan, after the hydrolysis of hair acid to produce cysteine, and use it as the high-salt amino acid wastewater required below. Its composition table is as follows:

[0027] Table 1 Contents of related elements in amino acid wastewater

[0028] project name

unit

Test results

Method basis

Total Nitrogen (N)

％

8.79

GB / T8572-2010

Sodium (Na)

％

0.52

ICP method

Chlorine (Cl)

g / L

131.26

AgCl nephelometric method

Lead (Pb)

mg / kg

0.91

ICP method

Arsenic (As)

mg / kg

0.076

Atomic Fluorescence

Mercury (Hg)

mg / kg

0.014

Atomic Fluorescence

Chromium (Cr)

mg / kg

1.51

ICP method

Cadmium (Cd)

mg / kg

0.06

ICP method

[0029] Table 2 Amino acid composition and content ...

Embodiment 2

[0037] Fermentation of brewer's yeast in liquid medium with glucose as carbon source and amino acid wastewater as nitrogen source.

[0038] 1. Prepare a liquid fermentation medium whose concentration of high-salt amino acid wastewater is 200g / L, wherein the high-salt amino acid wastewater is the only nitrogen source (its source and composition are consistent with Example 1), and the liquid fermentation medium is also added Other chemical substrates required by microorganisms, the components are: glucose: 50g / L, KH 2 PO 4 : 9g / L, yeast powder: 6g / L, MgSO 4 : 5g / L, CaCO 3 : 1.2g / L, FeSO 4 : 2.5g / L, the medium pH is 5.5.

[0039] 2. Inoculate the domesticated brewer's yeast strain obtained from Example 1 into the liquid fermentation medium prepared in step 1 according to the inoculum size of 2%, in the fermenter at 28°C, the rotating speed is 200rpm / min, and the aeration coefficient is 0.5 Under the conditions of liquid fermentation culture 5d. After the cultivation, the ce...

Embodiment 3

[0041] Fermentation of brewer's yeast in a liquid medium with cornstarch enzymatic hydrolyzate as carbon source and amino acid wastewater as nitrogen source.

[0042] 1. Prepare a liquid fermentation medium whose concentration of high-salt amino acid wastewater is 200g / L, wherein the high-salt amino acid wastewater is the only nitrogen source (its source and composition are consistent with Example 1), and the liquid fermentation medium is also added Other chemical substrates required by microorganisms, the components are: corn starch enzymatic hydrolysis solution 50g / L (according to the dry weight of corn starch), KH 2 PO 4 : 9g / L, yeast powder: 6g / L, MgSO 4 : 5g / L, CaCO 3 : 1.2g / L, FeSO 4 : 2.5g / L, the medium pH is 5.5.

[0043] 2. Inoculate the acclimated brewer's yeast strain obtained from Example 1 into the liquid fermentation medium prepared in step 1 according to the inoculum size of 5%, in the fermenter at 28°C, the rotating speed is 200rpm / min, and the aeration coe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing beer yeast for feeds by fermenting high-salt-content amino acid wastewater, which comprises the following steps: 1. habituated culture of feed strain: carrying out continuous habituated culture to enhance the tolerance of the strain to high-salt-content amino acid wastewater; and 2. fermentation of strain: under appropriate fermenting conditions, carrying out liquid ventilation culture in a fermentation tank for some time to obtain the microzyme for feeds. The microzyme is centrifugated, collected, dried in a 70 DEG C drying oven to constant weight, and weighed. The calculation indicates that every liter of high-salt-content amino acid wastewater can obtain 30g of beer yeast at the maximum. The method can effectively utilize amino acids, microelements and growth factors contained in the wastewater, greatly lowers the production cost of the beer yeast for feeds, greatly reduces the sewage treatment load, achieves the comprehensive utilization effect, and has obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of waste water resources, in particular to a method for producing beer yeast for feed by fermenting high-salt amino acid waste water. Background technique [0002] According to the World Health Organization, 70% of people in the world do not have access to safe and hygienic drinking water, and 25,000 people die every day from diseases related to sewage or poor sanitation; by 2050, it is estimated that there will be about 700,000 people in the world Millions of people face water shortages. 82% of the people in our country drink water from shallow wells and rivers, 75% of which are polluted by bacteria exceeding the hygienic standard, and the drinking water population polluted by organic matter is about 160 million. The sewage discharged by industry is the main source of water pollution. According to survey statistics, the national annual wastewater discharge is 65.92 billion tons. Among ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/16C12R1/865
Inventor 胡征杨波刘志刚
Owner HUBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap