Method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater

Abstract

The invention discloses a method for producing Bacillus subtilis for feeds by fermenting high-salt-content amino acid wastewater, which comprises the following steps: 1. habituated culture of feed strain: carrying out continuous habituated culture to enhance the tolerance of the strain to high-salt-content amino acid wastewater; and 2. fermentation of strain: under appropriate fermenting conditions, carrying out liquid ventilation culture in a fermentation tank for some time to obtain the Bacillus subtilis for feeds. The microzyme is centrifugated, collected, dried in a 70 DEG C drying oven to constant weight, and weighed. The calculation indicates that every liter of high-salt-content amino acid wastewater can obtain 35g of Bacillus subtilis at the maximum. The method can effectively utilize amino acids, microelements and growth factors contained in the wastewater, greatly lowers the fermenting cost of the Bacillus subtilis for feeds, greatly reduces the sewage treatment load, achieves the comprehensive utilization effect, and has obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of waste water resources, in particular to a method for fermenting high-salt amino acid waste water to produce Bacillus subtilis for feed. Background technique [0002] According to the World Health Organization, 70% of people in the world do not have access to safe and hygienic drinking water, and 25,000 people die every day from diseases related to sewage or poor sanitation; by 2050, it is estimated that there will be about 700,000 people in the world Millions of people face water shortages. 82% of the people in our country drink water from shallow wells and rivers, 75% of which are polluted by bacteria exceeding the hygienic standard, and the drinking water population polluted by organic matter is about 160 million. The sewage discharged by industry is the main source of water pollution. According to survey statistics, the national annual wastewater discharge is 65.92 billion tons. A...

AI Technical Summary

Problems solved by technology

Currently reported amino acid wastewater treatment methods mainly include electrodialysis, ultrafiltration, biochemical methods, etc., but the shortcomings of electrodialysis and ultrafiltration limit the promotion of this method due to high investment and operating costs and inconvenient maintenance; and biochemical methods Because of its low investment and operating costs, mature technology, no secondary pollution, and convenient operation and management, it has received worldwide attention in organic wastewater treatment.

The problem with this method at present is that it is more suitable for medium and low concentration sewage, but it is not suitable for treating high concentration amino acid wastewater

However, the whole treatment method has the disadvantages of high cost, time-consuming and labor-intensive concentration process, disconnection between resource recovery and wastewater treatment, and the fact that organic matter is easy to deteriorate after high-temperature evaporation and concentration.