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Preparation method of alpha-cyclodextrin

A cyclodextrin, pH value technology, applied in the field of biochemistry, can solve the problems of high equipment requirements, high production costs, complicated operations, etc., and achieve the effects of simple equipment requirements, reduced production costs, and simple preparation processes

Inactive Publication Date: 2014-03-19
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this invention is to provide a preparation method of α-cyclodextrin to overcome the high energy consumption, high equipment requirements and high risk in the prior art , complex operation and high production cost

Method used

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  • Preparation method of alpha-cyclodextrin

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preparation example Construction

[0020] The preparation principle of the present invention is to make a mixture of decyl alcohol and α-cyclodextrin by encapsulating α-cyclodextrin with decyl alcohol, and then use absolute ethanol to wash out decyl alcohol from α-cyclodextrin to make pure alpha-cyclodextrin. The decanol-absolute ethanol mixed solution washed out is recovered to ethanol, and the rest is decanol, which can be reused.

[0021] Raw materials and reagents in all following examples of the present invention are food grade, and its source and specification are as follows:

[0022]

[0023] In the embodiment, the detection of α-cyclodextrin content adopts high pressure liquid chromatography (the detection method comes from "China Food Additives" 2010.No.1Serial No.98(136-141) "High performance liquid chromatography for the determination of mixed ring α-cyclodextrin content in dextrin").

Embodiment 1

[0025] A kind of preparation method of α-cyclodextrin, comprises the following steps successively:

[0026] 1. Pretreatment: Add 50 grams of potato starch into 1000 ml of water under stirring conditions to make starch milk, adjust the pH value to 6.0, add 42 ml of cyclodextrin glucosyltransferase at 500 u per gram of dry starch, stir and heat up to 83 ±2°C for 10 minutes.

[0027] 2. Crude product generation: lower the temperature to 55±1°C, adjust the pH value to 5.0, add 58ml of cyclodextrin glucosyltransferase and 2.5ml of decanol according to 700u per gram of dry starch, and stir the reaction for 10 hours under this condition , stop responding.

[0028] 3. Refining: filter the reaction product of step 3 under airtight conditions, rinse the insoluble filter cake repeatedly with 1000 milliliters of water, collect the filter cake, and obtain 9.84 g of the filter cake. Add deionized water at a concentration of 5% to set the volume to 196.8ml, add 196.8ml of absolute ethanol,...

Embodiment 2

[0031] A kind of preparation method of α-cyclodextrin, comprises the following steps successively:

[0032] 1. Pretreatment: Add 50 grams of potato starch into 1000 ml of water under stirring conditions to make starch milk, adjust the pH value to 6.0, add 42 ml of cyclodextrin glucosyltransferase at 500 u per gram of dry starch, stir and heat up to 83 ±2°C for 10 minutes.

[0033] 2. Crude product generation: cool down to 55±1°C, adjust the pH value to 5.0, add 58ml of cyclodextrin glucosyltransferase and 5.0ml of decanol according to 700u per gram of dry starch, and stir for 10 hours under this condition , stop responding.

[0034] 3. Refining: the reaction product of step 3 was filtered under airtight conditions, and the insoluble matter filter cake was washed repeatedly with 1000 milliliters of water, and the filter cake was collected to obtain 12.8 g of the filter cake. Add deionized water at a concentration of 5% to set the volume to 256.0ml, add 256.0ml of absolute eth...

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Abstract

The invention relates to a preparation method of alpha-cyclodextrin. The prior art has the problems of high energy consumption, high equipment requirement, high dangerousness, complex operation and high production cost. The preparation method comprises the steps of: preparing starch into a starch emulsion, adding cyclodextrin glycosyltransferase, and stirring for liquidizing; supplementing the cyclodextrin glycosyltransferase, adding decyl alcohol, and stirring for reaction; filtering the mixture after the reaction is finished, collecting the insoluble substance, flushing, filtering, preparing into a solution, adding absolute ethyl alcohol, stirring and then standing; standing for layering, filtering the separated liquid, recovering ethanol from the solvent layer through an ethanol recovery device and then obtaining the left liquid, namely the decyl alcohol, and carrying out decolorization, concentration and spray-drying on the material liquid layer to obtain white powdery finished product alpha-cyclodextrin. According to the invention, the recovery rate of the refined alpha-cyclodextrin is higher than 95%, and the purity of the same is more than 99%; simultaneously, the preparation method of alpha-cyclodextrin is simple in preparation, high in production safety, low in cost and suitable for realizing large-scale production.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a preparation method of α-cyclodextrin. Background technique [0002] Due to its special molecular structure and characteristics, α-cyclodextrin has broad application prospects in the fields of food, medicine, cosmetics, pesticides, new materials, electronics, and environmental protection. [0003] α-Cyclodextrin (English name α-Cyclodextrin) is a new type of branched cyclodextrin that is linked by α-1-6 glycosidic bonds under the action of enzymes with starch as the substrate. α-cyclodextrin has a high solubility in the aqueous phase (25% at room temperature). And in the inclusion reaction, the concentration of the capsule core molecule that can be included is also increased accordingly, so as to obtain a better inclusion effect; more importantly, its safety is higher, so that α-cyclodextrin can be widely used in food, medicine , cosmetics, pesticides, new ma...

Claims

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Application Information

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IPC IPC(8): C12P19/18C08B37/16
Inventor 杨国武汪大敏李皎毛勇邓媛
Owner MICROBIOLOGY INST OF SHAANXI
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