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A method for extracting antioxidant active substances in rose leaves

A technology of antioxidant activity and extraction method is applied in the field of extraction of antioxidant active substances from rose leaves, which can solve the problems of inability to be widely promoted, high requirements for instruments and equipment, and high operating costs, and achieves broad industrialization prospects and low equipment investment costs. , the effect of high social and economic benefits

Active Publication Date: 2016-06-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is an extraction technology for extracting flavonoids from plants, but there are not many reports on extracting flavonoids from rose leaves at home and abroad. It is only found that Jia Changhong et al. use microwaves to extract rose leaf flavonoids, but this method is harmful to equipment. The requirements are high, the operating cost is high, and the application range is narrow, so it cannot be widely promoted. Therefore, there is no standardized product on the market.
[0004] In terms of the determination of antioxidant activity, the antioxidant activity of extracts on ABTS free radicals and DPPH free radicals can be quickly determined by using the scavenging ability of the extracts. However, these methods measure the total activity of the extracts, not a single component. Moreover, the method is determined by colorimetry, which is easily interfered by the substrate

Method used

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  • A method for extracting antioxidant active substances in rose leaves
  • A method for extracting antioxidant active substances in rose leaves
  • A method for extracting antioxidant active substances in rose leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] After the fresh rose leaves are dried in the sun (the water content is 5%), they are crushed and passed through a 20-mesh sieve to obtain rose leaf particles. Weigh 50g of rose leaf granules into the conical flask, add 500g of deionized water, extract at 70°C for 3 times, each time for 60min, and combine the obtained extracts. The combined extracts were centrifuged at 4500r / min for 15min to obtain a 1.5°Brix supernatant, which was concentrated under reduced pressure at 60°C to obtain a 6°Brix concentrate. Add ether, ethyl acetate, and water-saturated n-butanol solution to the concentrated solution in a volume ratio of 1:2, and extract them twice to obtain ether extract, ethyl acetate extract, n-butanol extract, and water extract. . The above extracts were respectively concentrated to dryness at 50°C under reduced pressure to obtain 2.36 g of ether extract (Fa), 3.85 g of ethyl acetate extract (Fb), 3.94 g of n-butanol extract (Fc), and water The extract (Fd) 2.97g was...

Embodiment 2

[0053] After the fresh rose leaves are dried in the sun (the water content is 6%), they are crushed and passed through a 20-mesh sieve to obtain rose leaf particles. After weighing 50g of rose leaf granules and adding them to the conical flask, add 1000g of ethanol aqueous solution with 50% ethanol volume percentage, extract twice at 70°C for 60min each time, and combine to obtain a 1.2°Brix extract. The extract was centrifuged at 4500r / min for 15min to obtain a supernatant, which was then concentrated under reduced pressure at 55°C to obtain a 6°Brix concentrate. Add ether, ethyl acetate, and water-saturated n-butanol solution to the concentrated solution in a volume ratio of 1:3, and extract once to obtain ether extract, ethyl acetate extract, n-butanol extract, and water extract in sequence. Each extract was concentrated to dryness under reduced pressure at 45°C to obtain ether extract (Fa) 2.12g, ethyl acetate extract (Fb) 3.89g, n-butanol extract (Fc) 3.57g, and water ext...

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Abstract

The invention discloses a method for extracting antioxidant active components from rose leaves. The method comprises the following steps: (1) drying fresh rose leaves in the sun, crushing and sieving the rose leaves to obtain rose leaf granules; (2) extracting the rose leaf granules with an extraction solvent to obtain an extract; (3) centrifuging the extract to obtain a supernatant and concentrating the supernatant to obtain a concentrated liquid; (4) extracting the concentrated liquid sequentially with ethyl ether, ethyl acetate and water saturated normal butanol solution to obtain an ethyl ether extract, an ethyl acetate extract, a normal butanol extract and a water extract respectively, then concentrating the extracts respectively to obtain an ethyl ether extractive, an ethyl acetate extractive, a normal butanol extractive and a water extractive, and respectively labeling the extractives as Fa, Fb, Fc and Fd, so as to obtain the antioxidant components extracted from rose leaves. The method for screening antioxidant active components provided by the invention employs products from separation of a free radical solution from a liquid chromatography column for online reaction, and the separation and screening are carried out continuously, so as to quickly detect the antioxidant active components.

Description

technical field [0001] The invention relates to a method for extracting antioxidant active substances in rose leaves. Background technique [0002] The human body will produce free radicals during normal metabolism or under various stress conditions. Excessive free radicals will lead to a series of diseases such as cancer, aging, and diabetes. At this time, it is necessary to supplement exogenous antioxidants to maintain the balance of free radicals in the body. Inhibiting the production of free radicals, quenching them quickly, and repairing the oxidative damage caused to the body is of great significance to human health. The search for natural antioxidant active ingredients and the determination of antioxidant activity have become a current hot spot. [0003] Rose leaves are the leaves of Rosaceae Rosa deciduous shrub rose, and rose leaves contain a large amount of flavonoids. In the antioxidant reaction, this kind of compound can not only scavenge the free radicals in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/738A61P39/06A61K127/00
Inventor 高彦祥王媛莉袁芳
Owner CHINA AGRI UNIV
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